F4/80 Monoclonal Antibody (BM8), Pacific Orange | Invitrogen MF48030 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

F4/80 Monoclonal Antibody (BM8), Pacific Orange

catalog :

MF48030

quantity :

1 mL

price :

US 491.00

clonality :

monoclonal

host :

rat

conjugate :

pacific orange

clone name :

BM8

reactivity :

mouse

application :

immunohistochemistry, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse	Pastille E, Bardini K, Fleissner D, Adamczyk A, Frede A, Wadwa M, et al. Transient ablation of regulatory T cells improves antitumor immunity in colitis-associated colon cancer. Cancer Res. 2014;74:4258-69 pubmed publisher
flow cytometry; mouse; fig 1	Chabtini L, Mfarrej B, Mounayar M, Zhu B, Batal I, Dakle P, et al. TIM-3 regulates innate immune cells to induce fetomaternal tolerance. J Immunol. 2013;190:88-96 pubmed publisher
flow cytometry; mouse	Pagán A, Pepper M, Chu H, Green J, Jenkins M. CD28 promotes CD4+ T cell clonal expansion during infection independently of its YMNM and PYAP motifs. J Immunol. 2012;189:2909-17 pubmed publisher
flow cytometry; mouse; fig 6	Wang Y, Szretter K, Vermi W, Gilfillan S, Rossini C, Cella M, et al. IL-34 is a tissue-restricted ligand of CSF1R required for the development of Langerhans cells and microglia. Nat Immunol. 2012;13:753-60 pubmed publisher
flow cytometry; mouse; fig 8	Shen J, Ren H, Tomiyama Miyaji C, Watanabe M, Kainuma E, Inoue M, et al. Resistance and augmentation of innate immunity in mice exposed to starvation. Cell Immunol. 2009;259:66-73 pubmed publisher
flow cytometry; mouse	Mangan N, Van Rooijen N, McKenzie A, Fallon P. Helminth-modified pulmonary immune response protects mice from allergen-induced airway hyperresponsiveness. J Immunol. 2006;176:138-47 pubmed
flow cytometry; mouse; fig 3	Noel J, Guo X, Wells Byrum D, Schwemberger S, Caldwell C, Ogle C. Effect of thermal injury on splenic myelopoiesis. Shock. 2005;23:115-22 pubmed
immunohistochemistry - frozen section; mouse; fig 9	Malorny U, Michels E, Sorg C. A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res. 1986;243:421-8 pubmed

product information

Product Type :

Antibody

Product Name :

F4/80 Monoclonal Antibody (BM8), Pacific Orange

Catalog # :

MF48030

Quantity :

1 mL

Price :

US 491.00

Clonality :

Monoclonal

Purity :

purified

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: Assay-dependent, Immunohistochemistry: Assay-dependent

Species :

Mouse

Clone :

BM8

Isotype :

IgG2a

Storage :

4 C, store in dark

Description :

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Immunogen :

mouse F4/80 which is a macrophage-restricted cell surface glycoprotein.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunohistochemistry: Assay-dependent

Aliases :

ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3

more info or order :

Invitrogen product webpage