F4/80 Monoclonal Antibody (BM8), Alexa Fluor 647 | Invitrogen MF48021 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

F4/80 Monoclonal Antibody (BM8), Alexa Fluor 647

catalog :

MF48021

quantity :

1 mL

price :

US 414.00

clonality :

monoclonal

host :

rat

conjugate :

AF647

clone name :

BM8

reactivity :

mouse

application :

flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; fig s3	Azcutia V, Kelm M, Luissint A, Boerner K, Flemming S, Quirós M, et al. Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo. Mucosal Immunol. 2020;: pubmed publisher
flow cytometry; mouse; 1:50; loading ...; fig 5a	Schmok E, Abad Dar M, Behrends J, Erdmann H, Rückerl D, Endermann T, et al. Suppressor of Cytokine Signaling 3 in Macrophages Prevents Exacerbated Interleukin-6-Dependent Arginase-1 Activity and Early Permissiveness to Experimental Tuberculosis. Front Immunol. 2017;8:1537 pubmed publisher
flow cytometry; mouse; loading ...; tbl 1	Pelletier M, Szymczak K, Barbeau A, Prata G, O Fallon K, Gaines P. Characterization of neutrophils and macrophages from ex vivo-cultured murine bone marrow for morphologic maturation and functional responses by imaging flow cytometry. Methods. 2017;112:124-146 pubmed publisher
flow cytometry; mouse; fig 4	Hsiao H, Thatcher T, Colas R, Serhan C, Phipps R, Sime P. Resolvin D1 Reduces Emphysema and Chronic Inflammation. Am J Pathol. 2015;185:3189-201 pubmed publisher
flow cytometry; mouse	Mangan N, Van Rooijen N, McKenzie A, Fallon P. Helminth-modified pulmonary immune response protects mice from allergen-induced airway hyperresponsiveness. J Immunol. 2006;176:138-47 pubmed
flow cytometry; mouse; fig 3	Noel J, Guo X, Wells Byrum D, Schwemberger S, Caldwell C, Ogle C. Effect of thermal injury on splenic myelopoiesis. Shock. 2005;23:115-22 pubmed
immunohistochemistry - frozen section; mouse; fig 9	Malorny U, Michels E, Sorg C. A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res. 1986;243:421-8 pubmed
	Griffin K, Mizenko R, Arun V, Carney R, Leach J. Extracellular Vesicles from Highly Metastatic Osteosarcoma Cells Induce Pro-Tumorigenic Macrophage Phenotypes. Adv Biol (Weinh). 2024;8:e2300577 pubmed publisher
	Deust A, Chobert M, Démontant V, Gricourt G, Denaës T, Thiolat A, et al. Macrophage autophagy protects against hepatocellular carcinogenesis in mice. Sci Rep. 2021;11:18809 pubmed publisher
	Giampazolias E, Schulz O, Lim K, Rogers N, Chakravarty P, Srinivasan N, et al. Secreted gelsolin inhibits DNGR-1-dependent cross-presentation and cancer immunity. Cell. 2021;184:4016-4031.e22 pubmed publisher
	Bencze G, Bencze S, Rivera K, Watson J, Hidvegi M, Orfi L, et al. Mito-oncology agent: fermented extract suppresses the Warburg effect, restores oxidative mitochondrial activity, and inhibits in vivo tumor growth. Sci Rep. 2020;10:14174 pubmed publisher
	YEKU O, Purdon T, Koneru M, Spriggs D, Brentjens R. Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment. Sci Rep. 2017;7:10541 pubmed publisher
	Egen J, Rothfuchs A, Feng C, Winter N, Sher A, Germain R. Macrophage and T cell dynamics during the development and disintegration of mycobacterial granulomas. Immunity. 2008;28:271-84 pubmed publisher

product information

Product Type :

Antibody

Product Name :

F4/80 Monoclonal Antibody (BM8), Alexa Fluor 647

Catalog # :

MF48021

Quantity :

1 mL

Price :

US 414.00

Clonality :

Monoclonal

Purity :

purified

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: Assay-dependent

Species :

Mouse

Clone :

BM8

Isotype :

IgG2a

Storage :

4 C

Description :

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Immunogen :

mouse F4/80 which is a macrophage-restricted cell surface glycoprotein.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent

Aliases :

ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3

more info or order :

Invitrogen product webpage