F4/80 Monoclonal Antibody (BM8), Alexa Fluor 488 | Invitrogen MF48020 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

F4/80 Monoclonal Antibody (BM8), Alexa Fluor 488

catalog :

MF48020

quantity :

1 mL

price :

US 309.00

clonality :

monoclonal

host :

rat

conjugate :

AF488

clone name :

BM8

reactivity :

human, mouse

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 35

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s2a	Al Zaeed N, Budai Z, Szondy Z, Sarang Z. TAM kinase signaling is indispensable for proper skeletal muscle regeneration in mice. Cell Death Dis. 2021;12:611 pubmed publisher
flow cytometry; mouse; loading ...; fig 6g	Petrovic I, Pejnovic N, Ljujic B, Pavlovic S, Miletic Kovacevic M, Jeftic I, et al. Overexpression of Galectin 3 in Pancreatic β Cells Amplifies β-Cell Apoptosis and Islet Inflammation in Type-2 Diabetes in Mice. Front Endocrinol (Lausanne). 2020;11:30 pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Giurisato E, Xu Q, Lonardi S, Telfer B, Russo I, Pearson A, et al. Myeloid ERK5 deficiency suppresses tumor growth by blocking protumor macrophage polarization via STAT3 inhibition. Proc Natl Acad Sci U S A. 2018;115:E2801-E2810 pubmed publisher
flow cytometry; mouse; 5:200; loading ...; fig 6a immunocytochemistry; mouse; 5:200; loading ...; fig 8a	Horsthemke M, Bachg A, Groll K, Moyzio S, MÃ¼ther B, Hemkemeyer S, et al. Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion. J Biol Chem. 2017;292:7258-7273 pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig 5b	Schumacher M, Hedl M, Abraham C, Bernard J, Lozano P, Hsieh J, et al. ErbB4 signaling stimulates pro-inflammatory macrophage apoptosis and limits colonic inflammation. Cell Death Dis. 2017;8:e2622 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig 4a	Henkels K, Muppani N, Gomez Cambronero J. PLD-Specific Small-Molecule Inhibitors Decrease Tumor-Associated Macrophages and Neutrophils Infiltration in Breast Tumors and Lung and Liver Metastases. PLoS ONE. 2016;11:e0166553 pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig st2	Rantakari P, Jäppinen N, Lokka E, Mokkala E, Gerke H, Peuhu E, et al. Fetal liver endothelium regulates the seeding of tissue-resident macrophages. Nature. 2016;538:392-396 pubmed publisher
immunohistochemistry; mouse; fig 3	Roy A, Femel J, Huijbers E, Spillmann D, Larsson E, Ringvall M, et al. Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma. PLoS ONE. 2016;11:e0156151 pubmed publisher
immunocytochemistry; human; 1:100; fig 2	McCarthy R, Lu D, Alkhateeb A, Gardeck A, Lee C, Wessling Resnick M. Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta. J Neuroinflammation. 2016;13:21 pubmed publisher
flow cytometry; mouse; fig 4	Hsiao H, Thatcher T, Colas R, Serhan C, Phipps R, Sime P. Resolvin D1 Reduces Emphysema and Chronic Inflammation. Am J Pathol. 2015;185:3189-201 pubmed publisher
flow cytometry; mouse	Jovicic N, Jeftic I, Jovanovic I, Radosavljevic G, Arsenijevic N, Lukic M, et al. Differential Immunometabolic Phenotype in Th1 and Th2 Dominant Mouse Strains in Response to High-Fat Feeding. PLoS ONE. 2015;10:e0134089 pubmed publisher
flow cytometry; mouse; fig 2	Shindo Y, Unsinger J, Burnham C, Green J, Hotchkiss R. Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression. Shock. 2015;43:334-43 pubmed publisher
immunohistochemistry; mouse; 1:50; fig 1	Rymo S, Gerhardt H, Wolfhagen Sand F, Lang R, Uv A, Betsholtz C. A two-way communication between microglial cells and angiogenic sprouts regulates angiogenesis in aortic ring cultures. PLoS ONE. 2011;6:e15846 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 2	HogenEsch H, Dunham A, Seymour R, Renninger M, Sundberg J. Expression of chitinase-like proteins in the skin of chronic proliferative dermatitis (cpdm/cpdm) mice. Exp Dermatol. 2006;15:808-14 pubmed
flow cytometry; mouse	Mangan N, Van Rooijen N, McKenzie A, Fallon P. Helminth-modified pulmonary immune response protects mice from allergen-induced airway hyperresponsiveness. J Immunol. 2006;176:138-47 pubmed
flow cytometry; mouse; fig 3	Noel J, Guo X, Wells Byrum D, Schwemberger S, Caldwell C, Ogle C. Effect of thermal injury on splenic myelopoiesis. Shock. 2005;23:115-22 pubmed
immunohistochemistry - frozen section; mouse; fig 9	Malorny U, Michels E, Sorg C. A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res. 1986;243:421-8 pubmed
	Tarban N, Papp A, De xe1 k D, Szentesi P, Hal xe1 sz H, Patsalos A, et al. Loss of adenosine A3 receptors accelerates skeletal muscle regeneration in mice following cardiotoxin-induced injury. Cell Death Dis. 2023;14:706 pubmed publisher
	Garabuczi x, Tarban N, Fige x, Patsalos A, Hal xe1 sz L, Szendi Szatm xe1 ri T, et al. Nur77 and PPARγ regulate transcription and polarization in distinct subsets of M2-like reparative macrophages during regenerative inflammation. Front Immunol. 2023;14:1139204 pubmed publisher
	Budai Z, Al Zaeed N, Szentesi P, Halász H, Csernoch L, Szondy Z, et al. Impaired Skeletal Muscle Development and Regeneration in Transglutaminase 2 Knockout Mice. Cells. 2021;10: pubmed publisher
	Zaidi Y, Corker A, Vasileva V, Oviedo K, Graham C, Wilson K, et al. Chronic Porphyromonas gingivalis lipopolysaccharide induces adverse myocardial infarction wound healing through activation of CD8+ T cells. Am J Physiol Heart Circ Physiol. 2021;321:H948-H962 pubmed publisher
	Walbaum S, Ambrosy B, Schütz P, Bachg A, Horsthemke M, Leusen J, et al. Complement receptor 3 mediates both sinking phagocytosis and phagocytic cup formation via distinct mechanisms. J Biol Chem. 2021;296:100256 pubmed publisher
	Mo Y, Zhang Y, Mo L, Wan R, Jiang M, Zhang Q. The role of miR-21 in nickel nanoparticle-induced MMP-2 and MMP-9 production in mouse primary monocytes: In vitro and in vivo studies. Environ Pollut. 2020;267:115597 pubmed publisher
	Kroeger C, Roesler R, Wiese S, Hainzl A, Gatzka M. Interaction of Deubiquitinase 2A-DUB/MYSM1 with DNA Repair and Replication Factors. Int J Mol Sci. 2020;21: pubmed publisher
	Ishimaru K, Yoshioka K, Kano K, Kurano M, Saigusa D, Aoki J, et al. Sphingosine kinase-2 prevents macrophage cholesterol accumulation and atherosclerosis by stimulating autophagic lipid degradation. Sci Rep. 2019;9:18329 pubmed publisher
	Sun D, Sun P, Li H, Zhang M, Liu G, Strickland A, et al. Fungal dissemination is limited by liver macrophage filtration of the blood. Nat Commun. 2019;10:4566 pubmed publisher
	Bachg A, Horsthemke M, Skryabin B, Klasen T, Nagelmann N, Faber C, et al. Phenotypic analysis of Myo10 knockout (Myo10tm2/tm2) mice lacking full-length (motorized) but not brain-specific headless myosin X. Sci Rep. 2019;9:597 pubmed publisher
	Zou A, Magee N, Deng F, Lehn S, Zhong C, Zhang Y. Hepatocyte nuclear receptor SHP suppresses inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis. J Biol Chem. 2018;293:8656-8671 pubmed publisher
	Peuhu E, Salomaa S, De Franceschi N, Potter C, Sundberg J, Pouwels J. Integrin beta 1 inhibition alleviates the chronic hyperproliferative dermatitis phenotype of SHARPIN-deficient mice. PLoS ONE. 2017;12:e0186628 pubmed publisher
	Chakraborty K, Raundhal M, Chen B, Morse C, Tyurina Y, Khare A, et al. The mito-DAMP cardiolipin blocks IL-10 production causing persistent inflammation during bacterial pneumonia. Nat Commun. 2017;8:13944 pubmed publisher
	Mani R, Amin M, Li X, Kalyana Sundaram S, Veeneman B, Wang L, et al. Inflammation-Induced Oxidative Stress Mediates Gene Fusion Formation in Prostate Cancer. Cell Rep. 2016;17:2620-2631 pubmed publisher
	Cipriani G, Gibbons S, Verhulst P, Choi K, Eisenman S, Hein S, et al. Diabetic Csf1op/op mice lacking macrophages are protected against the development of delayed gastric emptying. Cell Mol Gastroenterol Hepatol. 2016;2:40-47 pubmed
	Trikha P, Sharma N, Pena C, Reyes A, PÃ©cot T, Khurshid S, et al. E2f3 in tumor macrophages promotes lung metastasis. Oncogene. 2016;35:3636-46 pubmed publisher
	Smith K, Hochweller K, Hämmerling G, Boon L, MacDonald A, Maizels R. Chronic helminth infection promotes immune regulation in vivo through dominance of CD11cloCD103- dendritic cells. J Immunol. 2011;186:7098-109 pubmed publisher
	Jun D, Garat C, West J, Thorn N, Chow K, Cleaver T, et al. The pathology of bleomycin-induced fibrosis is associated with loss of resident lung mesenchymal stem cells that regulate effector T-cell proliferation. Stem Cells. 2011;29:725-35 pubmed publisher

product information

Product Type :

Antibody

Product Name :

F4/80 Monoclonal Antibody (BM8), Alexa Fluor 488

Catalog # :

MF48020

Quantity :

1 mL

Price :

US 309.00

Clonality :

Monoclonal

Purity :

purified

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: Assay-dependent

Species :

Mouse

Clone :

BM8

Isotype :

IgG2a

Storage :

4 C

Description :

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Immunogen :

mouse F4/80 which is a macrophage-restricted cell surface glycoprotein.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent

Aliases :

ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3

more info or order :

Invitrogen product webpage