product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
F4/80 Monoclonal Antibody (BM8), APC
catalog :
MF48005
quantity :
500 uL
price :
US 398.00
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
BM8
reactivity :
human, mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 38
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s3j
Bhattacharjee O, Ayyangar U, Kurbet A, Lakshmanan V, Palakodeti D, Ginhoux F, et al. Epithelial-Macrophage Crosstalk Initiates Sterile Inflammation in Embryonic Skin. Front Immunol. 2021;12:718005 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Chu C, Murdock M, Jing D, Won T, Chung H, Kressel A, et al. The microbiota regulate neuronal function and fear extinction learning. Nature. 2019;574:543-548 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Liu M, O Connor R, Trefely S, Graham K, Snyder N, Beatty G. Metabolic rewiring of macrophages by CpG potentiates clearance of cancer cells and overcomes tumor-expressed CD47-mediated 'don't-eat-me' signal. Nat Immunol. 2019;20:265-275 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1
Farhat K, Bodart G, Charlet Renard C, Desmet C, Moutschen M, Beguin Y, et al. Growth Hormone (GH) Deficient Mice With GHRH Gene Ablation Are Severely Deficient in Vaccine and Immune Responses Against Streptococcus pneumoniae. Front Immunol. 2018;9:2175 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 1h
Campana L, Starkey Lewis P, Pellicoro A, Aucott R, Man J, O Duibhir E, et al. The STAT3-IL-10-IL-6 Pathway Is a Novel Regulator of Macrophage Efferocytosis and Phenotypic Conversion in Sterile Liver Injury. J Immunol. 2018;200:1169-1187 pubmed publisher
  • flow cytometry; mouse; loading ...
Schweighoffer E, Nys J, Vanes L, Smithers N, Tybulewicz V. TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK. J Exp Med. 2017;214:1269-1280 pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...; fig 1b
Kumar A, Stoica B, Loane D, Yang M, Abulwerdi G, Khan N, et al. Microglial-derived microparticles mediate neuroinflammation after traumatic brain injury. J Neuroinflammation. 2017;14:47 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 5c
  • immunohistochemistry; mouse; 1:100; loading ...; fig 5a
Hasanov Z, Ruckdeschel T, König C, Mogler C, Kapel S, Korn C, et al. Endosialin Promotes Atherosclerosis Through Phenotypic Remodeling of Vascular Smooth Muscle Cells. Arterioscler Thromb Vasc Biol. 2017;37:495-505 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1A
Veinotte L, Gebremeskel S, Johnston B. CXCL16-positive dendritic cells enhance invariant natural killer T cell-dependent IFN? production and tumor control. Oncoimmunology. 2016;5:e1160979 pubmed publisher
  • flow cytometry; mouse
Kimmey J, Huynh J, Weiss L, Park S, Kambal A, Debnath J, et al. Unique role for ATG5 in neutrophil-mediated immunopathology during M. tuberculosis infection. Nature. 2015;528:565-9 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Lowe K, Navarro Núñez L, Bénézech C, Nayar S, Kingston B, Nieswandt B, et al. The expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state. Eur J Immunol. 2015;45:2484-93 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2
Bajwa A, Rosin D, Chrościcki P, Lee S, Dondeti K, Ye H, et al. Sphingosine 1-phosphate receptor-1 enhances mitochondrial function and reduces cisplatin-induced tubule injury. J Am Soc Nephrol. 2015;26:908-25 pubmed publisher
  • flow cytometry; mouse
Morganti J, Jopson T, Liu S, Gupta N, Rosi S. Cranial irradiation alters the brain's microenvironment and permits CCR2+ macrophage infiltration. PLoS ONE. 2014;9:e93650 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 1
Haldar M, Kohyama M, So A, Kc W, Wu X, Briseño C, et al. Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages. Cell. 2014;156:1223-1234 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 7
Xiao C, Feng R, Engevik A, Martin J, Tritschler J, Schumacher M, et al. Sonic Hedgehog contributes to gastric mucosal restitution after injury. Lab Invest. 2013;93:96-111 pubmed publisher
  • flow cytometry; human
Love W, Dobbs N, Tabor L, Simecka J. Toll-like receptor 2 (TLR2) plays a major role in innate resistance in the lung against murine Mycoplasma. PLoS ONE. 2010;5:e10739 pubmed publisher
  • flow cytometry; mouse
Leng J, Butcher B, Egan C, Abi Abdallah D, Denkers E. Toxoplasma gondii prevents chromatin remodeling initiated by TLR-triggered macrophage activation. J Immunol. 2009;182:489-97 pubmed
  • flow cytometry; mouse
Miyairi I, Tatireddigari V, Mahdi O, Rose L, Belland R, Lu L, et al. The p47 GTPases Iigp2 and Irgb10 regulate innate immunity and inflammation to murine Chlamydia psittaci infection. J Immunol. 2007;179:1814-24 pubmed
  • flow cytometry; mouse
Voehringer D, van Rooijen N, Locksley R. Eosinophils develop in distinct stages and are recruited to peripheral sites by alternatively activated macrophages. J Leukoc Biol. 2007;81:1434-44 pubmed
  • flow cytometry; mouse
Mangan N, Van Rooijen N, McKenzie A, Fallon P. Helminth-modified pulmonary immune response protects mice from allergen-induced airway hyperresponsiveness. J Immunol. 2006;176:138-47 pubmed
  • flow cytometry; mouse; fig 3
Noel J, Guo X, Wells Byrum D, Schwemberger S, Caldwell C, Ogle C. Effect of thermal injury on splenic myelopoiesis. Shock. 2005;23:115-22 pubmed
  • immunohistochemistry - frozen section; mouse; fig 9
Malorny U, Michels E, Sorg C. A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res. 1986;243:421-8 pubmed
Xu J, Shi C, Yuan F, Ding Y, Xie Y, Liu Y, et al. Targeted transplantation of engineered mitochondrial compound promotes functional recovery after spinal cord injury by enhancing macrophage phagocytosis. Bioact Mater. 2024;32:427-444 pubmed publisher
García Tejedor A, Haros C, Laparra Llopis J. Chenopodium quinoa's Ingredients Improve Control of the Hepatic Lipid Disturbances Derived from a High-Fat Diet. Foods. 2023;12: pubmed publisher
Lu G, Xu Y, Liu Q, Chen M, Sun H, Wang P, et al. An instantly fixable and self-adaptive scaffold for skull regeneration by autologous stem cell recruitment and angiogenesis. Nat Commun. 2022;13:2499 pubmed publisher
Oliva Ch xe1 vez A, Wang X, Marnin L, Archer N, Hammond H, Carroll E, et al. Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection. Nat Commun. 2021;12:3696 pubmed publisher
Devalaraja S, Haldar M. Intratumoral Monocyte Transfer to Examine Monocyte Differentiation in the Tumor Microenvironment. STAR Protoc. 2020;1:100188 pubmed publisher
Ma X, Zou F, Yu F, Li R, Yuan Y, Zhang Y, et al. Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses. Immunity. 2020;53:1315-1330.e9 pubmed publisher
Alam Z, Devalaraja S, Li M, To T, Folkert I, Mitchell Velasquez E, et al. Counter Regulation of Spic by NF-κB and STAT Signaling Controls Inflammation and Iron Metabolism in Macrophages. Cell Rep. 2020;31:107825 pubmed publisher
Kubinyecz O, Vikhe P, Purnell T, Brown S, Tateossian H. The Jeff Mouse Mutant Model for Chronic Otitis Media Manifests Gain-of-Function as Well as Loss-of-Function Effects. Front Genet. 2020;11:498 pubmed publisher
Devalaraja S, To T, Folkert I, Natesan R, Alam M, Li M, et al. Tumor-Derived Retinoic Acid Regulates Intratumoral Monocyte Differentiation to Promote Immune Suppression. Cell. 2020;180:1098-1114.e16 pubmed publisher
Long L, Yin M, Min W. 3D Co-culture System of Tumor-associated Macrophages and Ovarian Cancer Cells. Bio Protoc. 2018;8: pubmed publisher
Dai L, Bhargava P, Stanya K, Alexander R, Liou Y, Jacobi D, et al. Macrophage alternative activation confers protection against lipotoxicity-induced cell death. Mol Metab. 2017;6:1186-1197 pubmed publisher
Das S, Raundhal M, Chen J, Oriss T, Huff R, Williams J, et al. Respiratory syncytial virus infection of newborn CX3CR1-deficient mice induces a pathogenic pulmonary innate immune response. JCI Insight. 2017;2: pubmed publisher
Fletcher A, Elman J, Astarita J, Murray R, Saeidi N, D Rozario J, et al. Lymph node fibroblastic reticular cell transplants show robust therapeutic efficacy in high-mortality murine sepsis. Sci Transl Med. 2014;6:249ra109 pubmed publisher
Blaauboer S, Gabrielle V, Jin L. MPYS/STING-mediated TNF-α, not type I IFN, is essential for the mucosal adjuvant activity of (3'-5')-cyclic-di-guanosine-monophosphate in vivo. J Immunol. 2014;192:492-502 pubmed publisher
Bonne Annee S, Kerepesi L, Hess J, O Connell A, Lok J, Nolan T, et al. Human and mouse macrophages collaborate with neutrophils to kill larval Strongyloides stercoralis. Infect Immun. 2013;81:3346-55 pubmed publisher
Miyairi I, Laxton J, Wang X, Obert C, Arva Tatireddigari V, Van Rooijen N, et al. Chlamydia psittaci genetic variants differ in virulence by modulation of host immunity. J Infect Dis. 2011;204:654-63 pubmed publisher
product information
Product Type :
Antibody
Product Name :
F4/80 Monoclonal Antibody (BM8), APC
Catalog # :
MF48005
Quantity :
500 uL
Price :
US 398.00
Clonality :
Monoclonal
Purity :
purified
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: Assay-dependent
Species :
Mouse
Clone :
BM8
Isotype :
IgG2a
Storage :
4 C
Description :
F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Immunogen :
mouse F4/80 which is a macrophage-restricted cell surface glycoprotein.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: Assay-dependent
Aliases :
ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA