CD8 alpha Monoclonal Antibody (5H10), Pacific Orange | Invitrogen MCD0830 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD8 alpha Monoclonal Antibody (5H10), Pacific Orange

catalog :

MCD0830

quantity :

1 mL

price :

US 504.00

clonality :

monoclonal

host :

rat

conjugate :

pacific orange

clone name :

5H10

reactivity :

mouse

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 39

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s7c	Thompson P, Shah A, Ntranos V, Van Gool F, Atkinson M, Bhushan A. Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes. Cell Metab. 2019;29:1045-1060.e10 pubmed publisher
flow cytometry; mouse	Castro Rojas C, Deason K, Hussain R, Hayardeny L, Cravens P, Yarovinsky F, et al. Testing effects of glatiramer acetate and fingolimod in an infectious model of CNS immune surveillance. J Neuroimmunol. 2014;276:232-5 pubmed publisher
flow cytometry; mouse	Sreedharan R, Chen S, Miller M, Haribhai D, Williams C, Van Why S. Mice with an absent stress response are protected against ischemic renal injury. Kidney Int. 2014;86:515-24 pubmed publisher
flow cytometry; mouse	Murphy K, Erickson J, Johnson C, Seiler C, Bedi J, Hu P, et al. CD8+ T cell-independent tumor regression induced by Fc-OX40L and therapeutic vaccination in a mouse model of glioma. J Immunol. 2014;192:224-33 pubmed publisher
flow cytometry; mouse; fig 3	Schmitt E, Haribhai D, Jeschke J, Co D, Ziegelbauer J, Yan K, et al. Chronic follicular bronchiolitis requires antigen-specific regulatory T cell control to prevent fatal disease progression. J Immunol. 2013;191:5460-76 pubmed publisher
flow cytometry; mouse; fig 2	Bittner Eddy P, Fischer L, Costalonga M. Identification of gingipain-specific I-A(b) -restricted CD4+ T cells following mucosal colonization with Porphyromonas gingivalis in C57BL/6 mice. Mol Oral Microbiol. 2013;28:452-66 pubmed publisher
flow cytometry; mouse; fig 2	Fuertbauer E, Zaujec J, Uhrin P, Raab I, Weber M, Schachner H, et al. Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells. Immunol Lett. 2013;154:31-41 pubmed publisher
flow cytometry; mouse; fig 1	Cravens P, Kieseier B, Hussain R, Herndon E, Arellano B, Ben L, et al. The neonatal CNS is not conducive for encephalitogenic Th1 T cells and B cells during experimental autoimmune encephalomyelitis. J Neuroinflammation. 2013;10:67 pubmed publisher
flow cytometry; mouse	Hogan T, Shuvaev A, Commenges D, Yates A, Callard R, Thiebaut R, et al. Clonally diverse T cell homeostasis is maintained by a common program of cell-cycle control. J Immunol. 2013;190:3985-93 pubmed publisher
flow cytometry; mouse; fig 2	Sag D, Wingender G, Nowyhed H, Wu R, Gebre A, Parks J, et al. ATP-binding cassette transporter G1 intrinsically regulates invariant NKT cell development. J Immunol. 2012;189:5129-38 pubmed publisher
flow cytometry; mouse; fig 1	Pagán A, Pepper M, Chu H, Green J, Jenkins M. CD28 promotes CD4+ T cell clonal expansion during infection independently of its YMNM and PYAP motifs. J Immunol. 2012;189:2909-17 pubmed publisher
flow cytometry; mouse; fig 2	Hurez V, Daniel B, Sun L, Liu A, Ludwig S, Kious M, et al. Mitigating age-related immune dysfunction heightens the efficacy of tumor immunotherapy in aged mice. Cancer Res. 2012;72:2089-99 pubmed publisher
flow cytometry; mouse; fig 5	Lumsden J, Nurmukhambetova S, Klein J, Sattabongkot J, Bennett J, Bertholet S, et al. Evaluation of immune responses to a Plasmodium vivax CSP-based recombinant protein vaccine candidate in combination with second-generation adjuvants in mice. Vaccine. 2012;30:3311-9 pubmed publisher
flow cytometry; mouse	Sun L, Hurez V, Thibodeaux S, Kious M, Liu A, Lin P, et al. Aged regulatory T cells protect from autoimmune inflammation despite reduced STAT3 activation and decreased constraint of IL-17 producing T cells. Aging Cell. 2012;11:509-19 pubmed publisher
flow cytometry; mouse; fig 2	Salti S, Hammelev E, Grewal J, Reddy S, Zemple S, Grossman W, et al. Granzyme B regulates antiviral CD8+ T cell responses. J Immunol. 2011;187:6301-9 pubmed publisher
flow cytometry; mouse; fig 1	Kastenmuller W, Gasteiger G, Subramanian N, Sparwasser T, Busch D, Belkaid Y, et al. Regulatory T cells selectively control CD8+ T cell effector pool size via IL-2 restriction. J Immunol. 2011;187:3186-97 pubmed publisher
flow cytometry; mouse; fig s1	Sun J, Sukhova G, Zhang J, Chen H, Sjoberg S, Libby P, et al. Cathepsin K deficiency reduces elastase perfusion-induced abdominal aortic aneurysms in mice. Arterioscler Thromb Vasc Biol. 2012;32:15-23 pubmed publisher
flow cytometry; mouse; tbl 1	Zavitz C, Bauer C, Gaschler G, Fraser K, Strieter R, Hogaboam C, et al. Dysregulated macrophage-inflammatory protein-2 expression drives illness in bacterial superinfection of influenza. J Immunol. 2010;184:2001-13 pubmed publisher
flow cytometry; mouse; fig 5	Seavey M, Mosmann T. Estradiol-induced vaginal mucus inhibits antigen penetration and CD8(+) T cell priming in response to intravaginal immunization. Vaccine. 2009;27:2342-9 pubmed publisher
flow cytometry; mouse	Moon J, Chu H, Hataye J, Pagán A, Pepper M, McLachlan J, et al. Tracking epitope-specific T cells. Nat Protoc. 2009;4:565-81 pubmed publisher
flow cytometry; mouse; fig 2	Koehn B, Ford M, Ferrer I, Borom K, Gangappa S, Kirk A, et al. PD-1-dependent mechanisms maintain peripheral tolerance of donor-reactive CD8+ T cells to transplanted tissue. J Immunol. 2008;181:5313-22 pubmed
flow cytometry; mouse; fig 1	Willoughby J, Costello P, Nicolas R, Robinson N, Stamp G, Powrie F, et al. Raf signaling but not the ERK effector SAP-1 is required for regulatory T cell development. J Immunol. 2007;179:6836-44 pubmed
flow cytometry; mouse; fig 1	Choi E, Chen J, Wooldridge L, Salio M, Lissina A, Lissin N, et al. High avidity antigen-specific CTL identified by CD8-independent tetramer staining. J Immunol. 2003;171:5116-23 pubmed
flow cytometry; mouse; fig 4	Shinkai K, Mohrs M, Locksley R. Helper T cells regulate type-2 innate immunity in vivo. Nature. 2002;420:825-9 pubmed
	Jeon Y, Kang H, Yang Y, Park D, Choi B, Kim J, et al. A Novel Selective Axl/Mer/CSF1R Kinase Inhibitor as a Cancer Immunotherapeutic Agent Targeting Both Immune and Tumor Cells in the Tumor Microenvironment. Cancers (Basel). 2022;14: pubmed publisher
	Chryplewicz A, Scotton J, Tichet M, Zomer A, Shchors K, Joyce J, et al. Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity. Cancer Cell. 2022;40:1111-1127.e9 pubmed publisher
	Parigger T, Gassner F, Scherhäufl C, Bakar A, Höpner J, Hödlmoser A, et al. Evidence for Non-Cancer-Specific T Cell Exhaustion in the Tcl1 Mouse Model for Chronic Lymphocytic Leukemia. Int J Mol Sci. 2021;22: pubmed publisher
	Sun Y, Anyalebechi J, Sun H, Yumoto T, Xue M, Liu D, et al. Anti-TIGIT differentially affects sepsis survival in immunologically experienced versus previously naive hosts. JCI Insight. 2021;6: pubmed publisher
	Kamemura K, Chen C, Okumura M, Miura M, Chihara T. Amyotrophic lateral sclerosis-associated Vap33 is required for maintaining neuronal dendrite morphology and organelle distribution in Drosophila. Genes Cells. 2021;26:230-239 pubmed publisher
	Sun Y, Xie J, Anyalebechi J, Chen C, Sun H, Xue M, et al. CD28 Agonism Improves Survival in Immunologically Experienced Septic Mice via IL-10 Released by Foxp3+ Regulatory T Cells. J Immunol. 2020;205:3358-3371 pubmed publisher
	Kretschmer L, Flossdorf M, Mir J, Cho Y, Plambeck M, Treise I, et al. Differential expansion of T central memory precursor and effector subsets is regulated by division speed. Nat Commun. 2020;11:113 pubmed publisher
	Fay K, Chihade D, Chen C, Klingensmith N, Lyons J, Ramonell K, et al. Increased mortality in CD43-deficient mice during sepsis. PLoS ONE. 2018;13:e0202656 pubmed publisher
	Klingensmith N, Fay K, Lyons J, Chen C, Otani S, Liang Z, et al. Chronic Alcohol Ingestion Worsens Survival and Alters Gut Epithelial Apoptosis and CD8+ T Cell Function After Pseudomonas Aeruginosa Pneumonia-Induced Sepsis. Shock. 2019;51:453-463 pubmed publisher
	Publicover J, Gaggar A, Jespersen J, Halac U, JOHNSON A, Goodsell A, et al. An OX40/OX40L interaction directs successful immunity to hepatitis B virus. Sci Transl Med. 2018;10: pubmed publisher
	Catakovic K, Gassner F, Ratswohl C, Zaborsky N, Rebhandl S, Schubert M, et al. TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia. Oncoimmunology. 2017;7:e1371399 pubmed publisher
	Ramonell K, Zhang W, Hadley A, Chen C, Fay K, Lyons J, et al. CXCR4 blockade decreases CD4+ T cell exhaustion and improves survival in a murine model of polymicrobial sepsis. PLoS ONE. 2017;12:e0188882 pubmed publisher
	Prenek L, BoldizsÃ¡r F, Kugyelka R, Ugor E, Berta G, NÃ©meth P, et al. The regulation of the mitochondrial apoptotic pathway by glucocorticoid receptor in collaboration with Bcl-2 family proteins in developing T cells. Apoptosis. 2017;22:239-253 pubmed publisher
	Zhu M, Bakhru P, Conley B, Nelson J, Free M, Martin A, et al. Sex bias in CNS autoimmune disease mediated by androgen control of autoimmune regulator. Nat Commun. 2016;7:11350 pubmed publisher
	Pepper M, Linehan J, Pagán A, Zell T, Dileepan T, Cleary P, et al. Different routes of bacterial infection induce long-lived TH1 memory cells and short-lived TH17 cells. Nat Immunol. 2010;11:83-9 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD8 alpha Monoclonal Antibody (5H10), Pacific Orange

Catalog # :

MCD0830

Quantity :

1 mL

Price :

US 504.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: Assay-dependent

Species :

Mouse

Clone :

5H10

Isotype :

IgG2b

Storage :

4 C

Description :

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

Immunogen :

Con A-stimulated BALB/c splenic T cells

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent

Aliases :

BB154331; CD8; CD8 alpha; CD8 alpha chain; CD8 alpha chain precursor; CD8 antigen; CD8 antigen 32 kDa chain; CD8 antigen 37 kDa chain; CD8 antigen alpha polypeptide; CD8 antigen alpha protein; CD8 antigen alpha protein precursor; CD8 antigen alpha-chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8 antigen, alpha-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8 beta-2; CD8a; CD8A antigen alpha; CD8a molecule; CD8A; T-cell surface glycoprotein; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; Cd8b1; CD8beta; CD8BP; fCD8; LEU2; Leu-2; Leu2 T-lymphocyte antigen; leu-2a; Ly-2; LY3; Ly-3; Ly-35; Ly-B; Ly-C; Lymphocyte antigen 3; Lyt2; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt3; Lyt-3; MAL; membrane glycoprotein; membrane protein; OKT8 T-cell antigen; OX-8 membrane antigen; p32; P37; RHACD8-4; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T8 T-cell antigen; T-cell antigen Leu2; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3; T-cell surface molecule; T-lymphocyte differentiation antigen T8/Leu-2; type I transmembrane glycoprotein

more info or order :

Invitrogen product webpage