ICAM-1 Monoclonal Antibody (1A29) | Invitrogen MA5407 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ICAM-1 Monoclonal Antibody (1A29)

catalog :

MA5407

quantity :

500 µg

price :

US 450.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1A29

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; rat	Bedirli N, Akyurek N, Kurtipek O, Kavutcu M, Kartal S, Bayraktar A. Thoracic epidural bupivacaine attenuates inflammatory response, intestinal lipid peroxidation, oxidative injury, and mucosal apoptosis induced by mesenteric ischemia/reperfusion. Anesth Analg. 2011;113:1226-32 pubmed publisher
immunohistochemistry; rat; 1:50; fig 3 western blot; rat; 1:50; fig 5	Ito T, Kumamoto T, Horinouchi H, Yukishige K, Sugihara R, Fujimoto S, et al. Adhesion molecule expression in experimental myositis. Muscle Nerve. 2002;25:409-18 pubmed
	Radwan B, Prabhakaran A, Rocchetti S, Matuszyk E, Keyes T, Baranska M. Uptake and anti-inflammatory effects of liposomal astaxanthin on endothelial cells tracked by Raman and fluorescence imaging. Mikrochim Acta. 2023;190:332 pubmed publisher
	Hurtado P, Mart xed nez Pena I, Yepes Rodr xed guez S, Bascoy Otero M, Abu xed n C, Fern xe1 ndez Santiago C, et al. Modelling metastasis in zebrafish unveils regulatory interactions of cancer-associated fibroblasts with circulating tumour cells. Front Cell Dev Biol. 2023;11:1076432 pubmed publisher
	Sifat A, Archie S, Nozohouri S, Villalba H, Zhang Y, Sharma S, et al. Short-term exposure to JUUL electronic cigarettes can worsen ischemic stroke outcome. Fluids Barriers CNS. 2022;19:74 pubmed publisher
	Kögel A, Fikenzer S, Uhlmann L, Opitz L, Kneuer J, Haeusler K, et al. Extracellular Inflammasome Particles Are Released After Marathon Running and Induce Proinflammatory Effects in Endothelial Cells. Front Physiol. 2022;13:866938 pubmed publisher
	Walter F, Harazin A, T xf3 th A, Veszelka S, Santa Maria A, Barna L, et al. Blood-brain barrier dysfunction in L-ornithine induced acute pancreatitis in rats and the direct effect of L-ornithine on cultured brain endothelial cells. Fluids Barriers CNS. 2022;19:16 pubmed publisher
	Yue Q, Zhou X, Zhang Z, Hoi M. Murine Beta-Amyloid (1-42) Oligomers Disrupt Endothelial Barrier Integrity and VEGFR Signaling via Activating Astrocytes to Release Deleterious Soluble Factors. Int J Mol Sci. 2022;23: pubmed publisher
	You Y, Muraoka S, Jedrychowski M, Hu J, McQuade A, Young Pearse T, et al. Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain. J Extracell Vesicles. 2022;11:e12183 pubmed publisher
	Khan M, Qiao F, Kumar P, Touhidul Islam S, Singh A, Won J, et al. Neuroprotective effects of Alda-1 mitigate spinal cord injury in mice: involvement of Alda-1-induced ALDH2 activation-mediated suppression of reactive aldehyde mechanisms. Neural Regen Res. 2022;17:185-193 pubmed publisher
	Xu S, Bian H, Shu S, Xia S, Gu Y, Zhang M, et al. AIM2 deletion enhances blood-brain barrier integrity in experimental ischemic stroke. CNS Neurosci Ther. 2021;27:1224-1237 pubmed publisher
	Bhowmick S, Malat A, Caruso D, Ponery N, D Mello V, Finn C, et al. Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress. Eneuro. 2021;8: pubmed publisher
	Cao J, Wang B, Tang T, Wen Y, Li Z, Feng S, et al. Exosomal miR-125b-5p deriving from mesenchymal stem cells promotes tubular repair by suppression of p53 in ischemic acute kidney injury. Theranostics. 2021;11:5248-5266 pubmed publisher
	Offeddu G, Hajal C, Foley C, Wan Z, Ibrahim L, Coughlin M, et al. The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination. Commun Biol. 2021;4:255 pubmed publisher
	Khan M, Qiao F, Islam S, Dhammu T, Kumar P, Won J, et al. GSNOR and ALDH2 alleviate traumatic spinal cord injury. Brain Res. 2021;1758:147335 pubmed publisher
	Beddek K, Raffin F, Borgel D, Saller F, Riccobono D, Bobe R, et al. TRPV4 channel activation induces the transition of venous and arterial endothelial cells toward a pro-inflammatory phenotype. Physiol Rep. 2021;9:e14613 pubmed publisher
	Charan M, Das S, Mishra S, Chatterjee N, Varikuti S, Kaul K, et al. Macrophage migration inhibitory factor inhibition as a novel therapeutic approach against triple-negative breast cancer. Cell Death Dis. 2020;11:774 pubmed publisher
	Reyes L, Getachew H, Dunn W, Progulske Fox A. Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo. J Oral Microbiol. 2020;12:1742528 pubmed publisher
	Yokoyama S, Cai Y, Murata M, Tomita T, Yoneda M, Xu L, et al. A novel pathway of LPS uptake through syndecan-1 leading to pyroptotic cell death. elife. 2018;7: pubmed publisher
	Fu C, Yin D, Nie H, Sun D. Notoginsenoside R1 Protects HUVEC Against Oxidized Low Density Lipoprotein (Ox-LDL)-Induced Atherogenic Response via Down-Regulating miR-132. Cell Physiol Biochem. 2018;51:1739-1750 pubmed publisher
	Lo W, Yang W, Peng C, Pai W, Wang H. MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression. Front Physiol. 2018;9:355 pubmed publisher
	Bulk E, Kramko N, Liashkovich I, Glaser F, Schillers H, Schnittler H, et al. KCa3.1 channel inhibition leads to an ICAM-1 dependent increase of cell-cell adhesion between A549 lung cancer and HMEC-1 endothelial cells. Oncotarget. 2017;8:112268-112282 pubmed publisher
	Okumura S, Uemura T, Zhao X, Masano Y, Tsuruyama T, Fujimoto Y, et al. Liver graft preservation using perfluorocarbon improves the outcomes of simulated donation after cardiac death liver transplantation in rats. Liver Transpl. 2017;23:1171-1185 pubmed publisher
	Liu J, Yang F, Yang X, Jankowski M, Pagano P. NAD(P)H oxidase mediates angiotensin II-induced vascular macrophage infiltration and medial hypertrophy. Arterioscler Thromb Vasc Biol. 2003;23:776-82 pubmed
	Kiarash A, Pagano P, Tayeh M, Rhaleb N, Carretero O. Upregulated Expression of Rat Heart Intercellular Adhesion Molecule-1 in Angiotensin II- but Not Phenylephrine- Induced Hypertension. Hypertension. 2001;37:58-65 pubmed
	Yamazaki T, Seko Y, Tamatani T, Miyasaka M, Yagita H, Okumura K, et al. Expression of intercellular adhesion molecule-1 in rat heart with ischemia/reperfusion and limitation of infarct size by treatment with antibodies against cell adhesion molecules. Am J Pathol. 1993;143:410-8 pubmed
	Tamatani T, Kotani M, Miyasaka M. Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies. Eur J Immunol. 1991;21:627-33 pubmed

image

image 1 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse spleen tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing CD54 (MA5407) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 2 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing CD54 (MA5407) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 3 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lung tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing CD54 (MA5407) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

product information

Product Type :

Antibody

Product Name :

ICAM-1 Monoclonal Antibody (1A29)

Catalog # :

MA5407

Quantity :

500 µg

Price :

US 450.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Flow Cytometry: 1:20, Immunocytochemistry: 1:250, Immunohistochemistry (Paraffin): 1:100, Neutralization: Assay-dependent, Western Blot: 1:250

Species :

Human, Mouse, Rat

Clone :

1A29

Isotype :

IgG1

Storage :

-20°C

Description :

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

Immunogen :

Rat lymph node stroma

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:20, Immunocytochemistry: 1:250, Immunohistochemistry (Paraffin): 1:100, Neutralization: Assay-dependent, Western Blot: 1:250

Aliases :

BB2; CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM; Icam1; ICAM-1; ICAM-1; CD54 homolog; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; intercellular adhesion molecule-1 precursor; Ly 47; Ly-47; major group rhinovirus receptor; MALA2; MALA-2; MyD10; P3.58; sCD54; soluble CD54; Surface antigen of activated B cells

more info or order :

Invitrogen product webpage