ICAM-1 Monoclonal Antibody (3E2B) | Invitrogen MA5405 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ICAM-1 Monoclonal Antibody (3E2B)

catalog :

MA5405

quantity :

500 µg

price :

US 420.00

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

3E2B

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s8a	Anania J, Westin A, Adler J, Heyman B. A Novel Image Analysis Approach Reveals a Role for Complement Receptors 1 and 2 in Follicular Dendritic Cell Organization in Germinal Centers. Front Immunol. 2021;12:655753 pubmed publisher
	Wang C, Dong J, Sun J, Huang S, Wu F, Zhang X, et al. Silencing of lncRNA XIST impairs angiogenesis and exacerbates cerebral vascular injury after ischemic stroke. Mol Ther Nucleic Acids. 2021;26:148-160 pubmed publisher
	Zhang X, Wang L, Han Z, Dong J, Pang D, Fu Y, et al. KLF4 alleviates cerebral vascular injury by ameliorating vascular endothelial inflammation and regulating tight junction protein expression following ischemic stroke. J Neuroinflammation. 2020;17:107 pubmed publisher
	Merchant S, Gurule D, Larson R. Amelioration of ischemia-reperfusion injury with cyclic peptide blockade of ICAM-1. Am J Physiol Heart Circ Physiol. 2003;284:H1260-8 pubmed
	Lundberg A, Fukatsu K, Gaber L, Callicutt S, Kotb M, Wilcox H, et al. Blocking pulmonary ICAM-1 expression ameliorates lung injury in established diet-induced pancreatitis. Ann Surg. 2001;233:213-20 pubmed
	Ludwig R, Kretschmer M, Caspar G, Bojunga J, Oldenburg A, Schumm Draeger P, et al. In vivo microscopy of murine islets of Langerhans: increased adhesion of transferred lymphocytes to islets depends on macrophage-derived cytokines in a model of organ-specific insulitis. Immunology. 1999;98:111-5 pubmed
	Scheynius A, Camp R, Pure E. Reduced contact sensitivity reactions in mice treated with monoclonal antibodies to leukocyte function-associated molecule-1 and intercellular adhesion molecule-1. J Immunol. 1993;150:655-63 pubmed

image

image 1 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Hamster monoclonal antibody recognizing CD54 (MA5405) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 2 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lung tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a Hamster monoclonal antibody recognizing CD54 (MA5405) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 3 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse spleen tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Hamster monoclonal antibody recognizing CD54 (MA5405) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

product information

Product Type :

Antibody

Product Name :

ICAM-1 Monoclonal Antibody (3E2B)

Catalog # :

MA5405

Quantity :

500 µg

Price :

US 420.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Armenian Hamster

Reactivity :

Mouse

Applications :

ELISA: Assay-dependent, Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:10-1:100, Immunohistochemistry (Paraffin): 1:200, Inhibition Assays: Assay-dependent, Neutralization: Assay-dependent, Western Blot: 1:10-1:100

Species :

Mouse

Clone :

3E2B

Isotype :

IgG

Storage :

-20°C

Description :

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

Immunogen :

Mouse ICAM-1 (CD54)

Format :

Liquid

Applications w/Dilutions :

Aliases :

BB2; CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM; Icam1; ICAM-1; ICAM-1; CD54 homolog; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; intercellular adhesion molecule-1 precursor; Ly 47; Ly-47; major group rhinovirus receptor; MALA2; MALA-2; MyD10; P3.58; sCD54; soluble CD54; Surface antigen of activated B cells

more info or order :

Invitrogen product webpage