Cas9 Recombinant Rabbit Monoclonal Antibody (5C2Q1) | Invitrogen MA5-55402 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cas9 Recombinant Rabbit Monoclonal Antibody (5C2Q1)

catalog :

MA5-55402

quantity :

100 uL

price :

US 419.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

5C2Q1

reactivity :

human

application :

western blot, ELISA

product information

Product Type :

Antibody

Product Name :

Cas9 Recombinant Rabbit Monoclonal Antibody (5C2Q1)

Catalog # :

MA5-55402

Quantity :

100 uL

Price :

US 419.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Bacteria, Human

Applications :

ELISA: 1 ug/mL, Western Blot: 1:1,000-1:2,000

Species :

Bacteria, Human

Clone :

5C2Q1

Isotype :

IgG

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.

Immunogen :

Recombinant fusion protein containing a sequence corresponding to amino acids 450-730 of streptococcus pyogenes m1 Cas9.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1 ug/mL, Western Blot: 1:1,000-1:2,000

Aliases :

cas9; CRISPR9; CRISPR-Cas9; s aureus