DAAM1 Monoclonal Antibody (5D3) | Invitrogen MA5-47712 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

DAAM1 Monoclonal Antibody (5D3)

catalog :

MA5-47712

quantity :

100 uL

price :

US 454.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5D3

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

product information

Product Type :

Antibody

Product Name :

DAAM1 Monoclonal Antibody (5D3)

Catalog # :

MA5-47712

Quantity :

100 uL

Price :

US 454.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:50, Western Blot: 1:500

Species :

Human, Mouse, Rat

Clone :

5D3

Isotype :

IgG2a

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

DAAM1 is a diaphanous-related formin first studied as a novel Disheveled binding protein and shown to be crucial for the planar cell polarity pathway in Xenopus. DAAM1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. It is a Rho-regulated formin implicated in neuronal development and regulation of gastrulation. It was identified as a binding protein of the Wnt receptor-associated protein Disheveled. It also binds the GTP-bound RhoA and increases its GTP-bound form population in cells. It exists in an autoinhibited state via intramolecular interactions between its amino-terminal GBD and carboxyl-terminal DAD domains. Structurally the protein includes a GTPase-binding domain (GBD), a diaphanous inhibitory domain (DID), and a coiled-coil region followed by the FH1, FH2 and C-terminal diaphanous auto-regulatory domain (DAD). In humans, the DAAM1 gene is mapped to chromosome 14q23.1.

Immunogen :

Clone 5D3 was generated from a recombinant protein that included amino acid residues from the N-terminal region of human DAAM1. This sequence has less than 50% homology to the N-terminal region of DAAM2.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:50, Western Blot: 1:500

Aliases :

Daam1; Disheveled-associated activator of morphogenesis 1; dishevelled associated activator of morphogenesis 1; I79_021650; KIAA0666