This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
EGFR L858R Recombinant Rabbit Monoclonal Antibody (RM380), Biotin
catalog :
MA5-44558
quantity :
50 uL
price :
US 389.00
clonality :
recombinant
host :
domestic rabbit
conjugate :
biotin
clone name :
RM380

The same clone is also sold as:
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
product information
Product Type :
Antibody
Product Name :
EGFR L858R Recombinant Rabbit Monoclonal Antibody (RM380), Biotin
Catalog # :
MA5-44558
Quantity :
50 uL
Price :
US 389.00
Clonality :
Recombinant Monoclonal
Purity :
protein A
Host :
Rabbit
Reactivity :
Human
Applications :
Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:100-1:200, Western Blot: 1:1,000-1:2,000
Species :
Human
Clone :
RM380
Isotype :
IgG
Storage :
-20 C
Description :
EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. This product is specific for EGFR (mutant L858R).
Immunogen :
A peptide corresponding to human EGFR (L858R Mutant Specific). The immunogen range is a synthetic peptide within amino acids 1-100 of histone H3.3 with G34R mutant.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:100-1:200, Western Blot: 1:1,000-1:2,000
Aliases :
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EGFR; Epidermal growth factor receptor; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA