6x-His Tag Recombinant Rabbit Monoclonal Antibody (RM146) | Invitrogen MA5-33032 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

6x-His Tag Recombinant Rabbit Monoclonal Antibody (RM146)

catalog :

MA5-33032

quantity :

100 ug

price :

US 463.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

RM146

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 5

Reference
Park C, Hahn O, Gupta S, Moreno A, Marino F, Kedir B, et al. Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice. Nat Aging. 2023;3:1067-1078 pubmed publisher
Pudewell S, Lissy J, Nakhaeizadeh H, Mosaddeghzadeh N, Nakhaei Rad S, Dvorsky R, et al. New mechanistic insights into the RAS-SIN1 interaction at the membrane. Front Cell Dev Biol. 2022;10:987754 pubmed publisher
Kim J, Jozic A, Mukherjee A, Nelson D, Chiem K, Khan M, et al. Rapid Generation of Circulating and Mucosal Decoy Human ACE2 using mRNA Nanotherapeutics for the Potential Treatment of SARS-CoV-2. Adv Sci (Weinh). 2022;9:e2202556 pubmed publisher
Cottee M, Beckwith S, Letham S, Kim S, Young G, Stoye J, et al. Structure of a Ty1 restriction factor reveals the molecular basis of transposition copy number control. Nat Commun. 2021;12:5590 pubmed publisher
Kim J, Mukherjee A, Nelson D, Jozic A, Sahay G. Rapid generation of circulating and mucosal decoy ACE2 using mRNA nanotherapeutics for the potential treatment of SARS-CoV-2. bioRxiv. 2020;: pubmed publisher

product information

Product Type :

Antibody

Product Name :

6x-His Tag Recombinant Rabbit Monoclonal Antibody (RM146)

Catalog # :

MA5-33032

Quantity :

100 ug

Price :

US 463.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Tag

Applications :

Flow Cytometry: 0.5-2 ug/mL, Immunocytochemistry: 0.5-2 ug/mL, Immunohistochemistry: 0.1-1 ug/mL, Immunoprecipitation: 0.5-2 ug/mL, Western Blot: 0.1-1 ug/mL

Species :

Tag

Clone :

RM146

Isotype :

IgG

Storage :

-20 C

Description :

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Immunogen :

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5-2 ug/mL, Immunocytochemistry: 0.5-2 ug/mL, Immunohistochemistry: 0.1-1 ug/mL, Immunoprecipitation: 0.5-2 ug/mL, Western Blot: 0.1-1 ug/mL

Aliases :

6His tag; 6X His; His Tag; Histidine Tag