KLH Monoclonal Antibody (15F7E4G3) | Invitrogen MA5-28972 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

KLH Monoclonal Antibody (15F7E4G3)

catalog :

MA5-28972

quantity :

100 uL

price :

US 323.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

15F7E4G3

application :

western blot, ELISA

citations: 1

Reference
Montégut L, Chen H, Bravo San Pedro J, Motiño O, Martins I, Kroemer G. Immunization of mice with the self-peptide ACBP coupled to keyhole limpet hemocyanin. STAR Protoc. 2022;3:101095 pubmed publisher

product information

Product Type :

Antibody

Product Name :

KLH Monoclonal Antibody (15F7E4G3)

Catalog # :

MA5-28972

Quantity :

100 uL

Price :

US 323.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Tag

Applications :

ELISA: 1:1,000-1:2,000, Western Blot: 1:500-1:1,000

Species :

Tag

Clone :

15F7E4G3

Isotype :

IgG1

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Keyhole limpet hemocyanin is an extremely large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000 in aggregates with molecular weights of 4,500,000-13,000,000. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata.Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of for research, biotechnology and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production.[2] KLH is the most widely employed carrier proteins for this purpose. KLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens, allows a high hapten:carrier protein ratio increasing the likelihood of generating hapten-specific . In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically based research techniques in mammalian model organisms. KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate. Aggregates remain immunogenic, but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory.

Immunogen :

Recombinant KLH protein

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:1,000-1:2,000, Western Blot: 1:500-1:1,000

Aliases :

keyhole limpet hemocyanin