This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
HSF1 Monoclonal Antibody (10H8)
catalog :
MA5-27688
quantity :
100 ug
price :
US 504.00
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
10H8

The same clone is also sold as:
reactivity :
guinea pig, Chinese hamsters, human, mouse, rat, bovine, domestic rabbit, rhesus macaque
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 1
Reference
Dong Q, Xiu Y, Wang Y, Hodgson C, Borcherding N, Jordan C, et al. HSF1 is a driver of leukemia stem cell self-renewal in acute myeloid leukemia. Nat Commun. 2022;13:6107 pubmed publisher
product information
Product Type :
Antibody
Product Name :
HSF1 Monoclonal Antibody (10H8)
Catalog # :
MA5-27688
Quantity :
100 ug
Price :
US 504.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Rat
Reactivity :
Bovine, Guinea Pig, Hamster, Human, Mouse, Rabbit, Rat, Rhesus Monkey
Applications :
ELISA: Assay-Dependent, Gel Shift: Assay-dependent, Immunocytochemistry: 1:200, Immunohistochemistry (PFA fixed): 1:1,000, Immunoprecipitation: Assay-Dependent, Western Blot: 1:1,000
Species :
Bovine, Guinea Pig, Hamster, Human, Mouse, Rabbit, Rat, Rhesus Monkey
Clone :
10H8
Isotype :
IgG1
Storage :
-20 C
Description :
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
Immunogen :
Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-Dependent, Gel Shift: Assay-dependent, Immunocytochemistry: 1:200, Immunohistochemistry (PFA fixed): 1:1,000, Immunoprecipitation: Assay-Dependent, Western Blot: 1:1,000
Aliases :
AA960185; heat shock factor 1; heat shock factor 2; Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF; HSF 1; Hsf1; Hsf1alpha; Hsf1beta; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1; HSTF1
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA