Estrogen Receptor Beta Monoclonal Antibody (PPZ0506) | Invitrogen MA5-24807 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Estrogen Receptor Beta Monoclonal Antibody (PPZ0506)

catalog :

MA5-24807

quantity :

100 µL

price :

US 396.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PPZ0506

reactivity :

human, mouse

application :

western blot, ELISA, immunoprecipitation, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; loading ...; fig 1c	Yuan B, Yang J, Dubeau L, Hu Y, Li R. A Phosphotyrosine Switch in Estrogen Receptor β Is Required for Mouse Ovarian Function. Front Cell Dev Biol. 2021;9:649087 pubmed publisher
western blot; human; 1:500; loading ...; fig 1	Treeck O, Diepolder E, Skrzypczak M, Schüler Toprak S, Ortmann O. Knockdown of estrogen receptor β increases proliferation and affects the transcriptome of endometrial adenocarcinoma cells. BMC Cancer. 2019;19:745 pubmed publisher
	Yuan B, Clark C, Wu B, Yang J, Drerup J, Li T, et al. Estrogen receptor beta signaling in CD8+ T cells boosts T cell receptor activation and antitumor immunity through a phosphotyrosine switch. J Immunother Cancer. 2021;9: pubmed publisher
	Alexandrova E, Lamberti J, Saggese P, Pecoraro G, Memoli D, Cappa V, et al. Small Non-Coding RNA Profiling Identifies miR-181a-5p as a Mediator of Estrogen Receptor Beta-Induced Inhibition of Cholesterol Biosynthesis in Triple-Negative Breast Cancer. Cells. 2020;9: pubmed publisher
	Alexandrova E, Giurato G, Saggese P, Pecoraro G, Lamberti J, Ravo M, et al. Interaction Proteomics Identifies ERbeta Association with Chromatin Repressive Complexes to Inhibit Cholesterol Biosynthesis and Exert An Oncosuppressive Role in Triple-negative Breast Cancer. Mol Cell Proteomics. 2020;19:245-260 pubmed publisher
	Hawse J, Carter J, Aspros K, Bruinsma E, Koepplin J, Negron V, et al. Optimized immunohistochemical detection of estrogen receptor beta using two validated monoclonal antibodies confirms its expression in normal and malignant breast tissues. Breast Cancer Res Treat. 2020;179:241-249 pubmed publisher
	Ambhore N, Katragadda R, Raju Kalidhindi R, Thompson M, Pabelick C, Prakash Y, et al. Estrogen receptor beta signaling inhibits PDGF induced human airway smooth muscle proliferation. Mol Cell Endocrinol. 2018;476:37-47 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Estrogen Receptor Beta Monoclonal Antibody (PPZ0506)

Catalog # :

MA5-24807

Quantity :

100 µL

Price :

US 396.00

Clonality :

Monoclonal

Purity :

Ammonium sulfate precipitation

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: 0.2 µg/mL, Immunoprecipitation: Assay-Dependent, Western Blot: 1 µg/mL

Species :

Human

Clone :

PPZ0506

Isotype :

IgG2b

Storage :

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Description :

Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.

Immunogen :

Estrogen Receptor beta antibody for 2-88 amino acid peptide in human ER beta. Ligand for estrogenic 17b-estradiol compound.

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.2 µg/mL, Immunoprecipitation: Assay-Dependent, Western Blot: 1 µg/mL

Aliases :

ER; ER beta; ER[b]; Erb; Erb2; Erbeta; ER-beta; Esr2; ESRB; ESR-BETA; ESTRB; estrogen receptor 2; estrogen receptor 2 (beta); estrogen receptor 2 (ER beta); estrogen receptor 2 beta; estrogen receptor beta; estrogen receptor beta 1 isoform deletion variant; estrogen receptor beta 4; NR3A2; Nuclear receptor subfamily 3 group A member 2; oestrogen receptor beta; steroid receptor

more info or order :

Invitrogen product webpage