Cas9 Monoclonal Antibody (7A9) | Invitrogen MA5-23519 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cas9 Monoclonal Antibody (7A9)

catalog :

MA5-23519

quantity :

50 ug

price :

US 582.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7A9

reactivity :

Streptococcus pyogenes

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; Streptococcus pyogenes; 1:1000; loading ...; fig s3a	Choi E, Suh S, Foik A, Leinonen H, Newby G, Gao X, et al. In vivo base editing rescues cone photoreceptors in a mouse model of early-onset inherited retinal degeneration. Nat Commun. 2022;13:1830 pubmed publisher
	Li Y, Adur M, Wang W, Schultz R, Hale B, Wierson W, et al. Effect of ARTEMIS (DCLRE1C) deficiency and microinjection timing on editing efficiency during somatic cell nuclear transfer and in vitro fertilization using the CRISPR/Cas9 system. Theriogenology. 2021;170:107-116 pubmed publisher
	Hunt C, Hartford S, White D, Pefanis E, Hanna T, Herman C, et al. Tissue-specific activation of gene expression by the Synergistic Activation Mediator (SAM) CRISPRa system in mice. Nat Commun. 2021;12:2770 pubmed publisher
	Levy J, Yeh W, Pendse N, Davis J, Hennessey E, Butcher R, et al. Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020;4:97-110 pubmed publisher
	Brinkman E, Chen T, de Haas M, Holland H, Akhtar W, van Steensel B. Kinetics and Fidelity of the Repair of Cas9-Induced Double-Strand DNA Breaks. Mol Cell. 2018;70:801-813.e6 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Cas9 Monoclonal Antibody (7A9)

Catalog # :

MA5-23519

Quantity :

50 ug

Price :

US 582.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bacteria

Applications :

Immunocytochemistry: 1:100-1:500, Immunoprecipitation: 1:200, Western Blot: 1:1,000-1:6,000

Species :

Bacteria

Clone :

7A9

Isotype :

IgG1, kappa

Storage :

-20 C or -80 C if preferred

Description :

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.

Immunogen :

Cas9 nuclease (CRISPR-associated protein 9).

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:500, Immunoprecipitation: 1:200, Western Blot: 1:1,000-1:6,000

Aliases :

cas9; CRISPR9; CRISPR-Cas9; s aureus

more info or order :

Invitrogen product webpage