CD11b Monoclonal Antibody (M1/70.15), FITC | Invitrogen MA5-17859 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD11b Monoclonal Antibody (M1/70.15), FITC

catalog :

MA5-17859

quantity :

300 ug

price :

US 372.00

clonality :

monoclonal

host :

rat

conjugate :

FITC

clone name :

M1/70.15

reactivity :

mouse

application :

immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse	Siegemund S, Hartl A, von Buttlar H, Dautel F, Raue R, Freudenberg M, et al. Conventional bone marrow-derived dendritic cells contribute to toll-like receptor-independent production of alpha/beta interferon in response to inactivated parapoxvirus ovis. J Virol. 2009;83:9411-22 pubmed publisher
flow cytometry; mouse	Silver K, Ferry H, Crockford T, Cornall R. TLR4, TLR9 and MyD88 are not required for the positive selection of autoreactive B cells into the primary repertoire. Eur J Immunol. 2006;36:1404-12 pubmed
flow cytometry; mouse; fig 7	Haque A, Easton A, Smith D, O GARRA A, Van Rooijen N, Lertmemongkolchai G, et al. Role of T cells in innate and adaptive immunity against murine Burkholderia pseudomallei infection. J Infect Dis. 2006;193:370-9 pubmed
flow cytometry; mouse	Gomariz R, Arranz A, Abad C, Torroba M, Martinez C, Rosignoli F, et al. Time-course expression of Toll-like receptors 2 and 4 in inflammatory bowel disease and homeostatic effect of VIP. J Leukoc Biol. 2005;78:491-502 pubmed
flow cytometry; mouse; fig 1b	Cavassani K, Aliberti J, Dias A, Silva J, Ferreira B. Tick saliva inhibits differentiation, maturation and function of murine bone-marrow-derived dendritic cells. Immunology. 2005;114:235-45 pubmed
flow cytometry; mouse; fig 5	Ohse T, Ota T, Kieran N, Godson C, Yamada K, Tanaka T, et al. Modulation of interferon-induced genes by lipoxin analogue in anti-glomerular basement membrane nephritis. J Am Soc Nephrol. 2004;15:919-27 pubmed
flow cytometry; mouse	Harris C, Hanna S, Mizuno M, Holt D, Marchbank K, Morgan B. Characterization of the mouse analogues of CD59 using novel monoclonal antibodies: tissue distribution and functional comparison. Immunology. 2003;109:117-26 pubmed
flow cytometry; mouse	Angulo I, Jiménez Díaz M, García Bustos J, Gargallo D, de las Heras F, Muñoz Fernández M, et al. Candida albicans infection enhances immunosuppression induced by cyclophosphamide by selective priming of suppressive myeloid progenitors for NO production. Cell Immunol. 2002;218:46-58 pubmed
flow cytometry; mouse; fig 4	Goñi O, Alcaide P, Fresno M. Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+))CD11b(+ )immature myeloid suppressor cells. Int Immunol. 2002;14:1125-34 pubmed
flow cytometry; mouse; 0.5 ug/ml	Denda Nagai K, Kubota N, Tsuiji M, Kamata M, Irimura T. Macrophage C-type lectin on bone marrow-derived immature dendritic cells is involved in the internalization of glycosylated antigens. Glycobiology. 2002;12:443-50 pubmed
flow cytometry; mouse	Angulo I, de las Heras F, Garcia Bustos J, Gargallo D, Munoz Fernandez M, Fresno M. Nitric oxide-producing CD11b(+)Ly-6G(Gr-1)(+)CD31(ER-MP12)(+) cells in the spleen of cyclophosphamide-treated mice: implications for T-cell responses in immunosuppressed mice. Blood. 2000;95:212-20 pubmed
flow cytometry; mouse	Dejbakhsh Jones S, Strober S. Identification of an early T cell progenitor for a pathway of T cell maturation in the bone marrow. Proc Natl Acad Sci U S A. 1999;96:14493-8 pubmed
flow cytometry; mouse	Masurier C, Pioche Durieu C, Colombo B, Lacave R, Lemoine F, Klatzmann D, et al. Immunophenotypical and functional heterogeneity of dendritic cells generated from murine bone marrow cultured with different cytokine combinations: implications for anti-tumoral cell therapy. Immunology. 1999;96:569-77 pubmed
flow cytometry; mouse; fig 2, 3	Clark L, Appleby M, Brunkow M, Wilkinson J, Ziegler S, Ramsdell F. Cellular and molecular characterization of the scurfy mouse mutant. J Immunol. 1999;162:2546-54 pubmed
flow cytometry; mouse	Showell H, Conklyn M, Alpert R, Hingorani G, Wright K, Smith M, et al. The preclinical pharmacological profile of the potent and selective leukotriene B4 antagonist CP-195543. J Pharmacol Exp Ther. 1998;285:946-54 pubmed
flow cytometry; mouse	Guttormsen H, Wetzler L, Finberg R, Kasper D. Immunologic memory induced by a glycoconjugate vaccine in a murine adoptive lymphocyte transfer model. Infect Immun. 1998;66:2026-32 pubmed
flow cytometry; mouse	Salomon B, Cohen J, Masurier C, Klatzmann D. Three populations of mouse lymph node dendritic cells with different origins and dynamics. J Immunol. 1998;160:708-17 pubmed
flow cytometry; mouse; fig 5	Ushio Umeda Y, Yoshida R. Mechanisms of allografted tumor rejection: the roles of T cells in allograft rejection mediated by a type of bone marrow-derived macrophage. Microbiol Immunol. 1997;41:981-90 pubmed
flow cytometry; mouse; fig 1	Mukouyama H, Tanabe M. Thy.1lowCD3- cells sorted from nylon wool-passed bone marrow cells can augment the H-2 identical but not non-identical cytotoxic T lymphocyte precursors in mixed lymphocyte cultures. Microbiol Immunol. 1994;38:957-65 pubmed
flow cytometry; mouse	Yoshida R, Takikawa O, Oku T, Habara Ohkubo A. Mononuclear phagocytes: a major population of effector cells responsible for rejection of allografted tumor cells in mice. Proc Natl Acad Sci U S A. 1991;88:1526-30 pubmed
flow cytometry; mouse; tbl 2	Schuyler M, Gott K, Shopp G, Crooks L. CD3+ and CD4+ cells adoptively transfer experimental hypersensitivity pneumonitis. Am Rev Respir Dis. 1992;146:1582-8 pubmed
	Liu W, Chen W, Xie M, Chen C, Shao Z, Zhang Y, et al. Traumatic brain injury stimulates sympathetic tone-mediated bone marrow myelopoiesis to favor fracture healing. Signal Transduct Target Ther. 2023;8:260 pubmed publisher
	Weng Q, Hu F, Zhang M, Dong Y, Lv C, Wang Y, et al. A protocol for generating induced T cells by reprogramming B cells in vivo. Cell Regen (Lond). 2018;7:7-15 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD11b Monoclonal Antibody (M1/70.15), FITC

Catalog # :

MA5-17859

Quantity :

300 ug

Price :

US 372.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 0.2-0.5 ug/1x10^6 cells, Immunocytochemistry: Assay-dependent

Species :

Mouse

Clone :

M1/70.15

Isotype :

IgG2b

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles. Store in the dark.

Description :

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

Immunogen :

C57BL/6 spleen cell enriched for T lymphocytes

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.2-0.5 ug/1x10^6 cells, Immunocytochemistry: Assay-dependent

Aliases :

antigen CD11b (p170); antigen CD11b (p170); macrophage antigen alpha polypeptide; CD11 antigen-like family member B; CD11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha M; integrin alpha-M; integrin subunit alpha M; integrin, alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; leukocyte integrin alpha-M chain; LOC100351865; Ly-40; MAC1; Mac-1; Mac-1 alpha; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6; unnamed protein product

more info or order :

Invitrogen product webpage