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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD11b Monoclonal Antibody (M1/70.15), Biotin
catalog :
MA5-17858
quantity :
300 ug
price :
US 414.00
clonality :
monoclonal
host :
rat
conjugate :
biotin
clone name :
M1/70.15
reactivity :
mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 10
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; fig 1
Wainwright D, Sengupta S, Han Y, Ulasov I, Lesniak M. The presence of IL-17A and T helper 17 cells in experimental mouse brain tumors and human glioma. PLoS ONE. 2010;5:e15390 pubmed publisher
  • flow cytometry; mouse
Velaga S, Herbrand H, Friedrichsen M, Jiong T, Dorsch M, Hoffmann M, et al. Chemokine receptor CXCR5 supports solitary intestinal lymphoid tissue formation, B cell homing, and induction of intestinal IgA responses. J Immunol. 2009;182:2610-9 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 1f
Sato K, Komatsu N, Higashi N, Imai Y, Irimura T. Granulation tissue formation by nonspecific inflammatory agent occurs independently of macrophage galactose-type C-type lectin-1. Clin Immunol. 2005;115:47-50 pubmed
  • flow cytometry; mouse; 1 ug/ml
Sato K, Imai Y, Higashi N, Kumamoto Y, Onami T, Hedrick S, et al. Lack of antigen-specific tissue remodeling in mice deficient in the macrophage galactose-type calcium-type lectin 1/CD301a. Blood. 2005;106:207-15 pubmed
  • flow cytometry; mouse; fig 4
Lan F, Zeng D, Higuchi M, Higgins J, Strober S. Host conditioning with total lymphoid irradiation and antithymocyte globulin prevents graft-versus-host disease: the role of CD1-reactive natural killer T cells. Biol Blood Marrow Transplant. 2003;9:355-63 pubmed
  • flow cytometry; mouse; fig 1, 2
Lan F, Zeng D, Higuchi M, Huie P, Higgins J, Strober S. Predominance of NK1.1+TCR alpha beta+ or DX5+TCR alpha beta+ T cells in mice conditioned with fractionated lymphoid irradiation protects against graft-versus-host disease: "natural suppressor" cells. J Immunol. 2001;167:2087-96 pubmed
  • flow cytometry; mouse; fig 6
Tourneur L, Malassagne B, Batteux F, Fabre M, Mistou S, Lallemand E, et al. Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts. J Immunol. 2001;167:1338-46 pubmed
  • flow cytometry; mouse; fig 3
Stickler M, Harding F. Peritoneal B-cell development depends on strain, radiation, and time. Exp Hematol. 2001;29:221-7 pubmed
  • flow cytometry; mouse
Barrat F, Lesourd B, Louise A, Boulouis H, Vincent Naulleau S, Thibault D, et al. Surface antigen expression in spleen cells of C57B1/6 mice during ageing: influence of sex and parity. Clin Exp Immunol. 1997;107:593-600 pubmed
Lu L, Li T, Feng X, Liu Z, Liu Y, Chao T, et al. Excessive immunosuppression by regulatory T cells antagonizes T cell response to schistosome infection in PD-1-deficient mice. PLoS Pathog. 2022;18:e1010596 pubmed publisher
product information
Product Type :
Antibody
Product Name :
CD11b Monoclonal Antibody (M1/70.15), Biotin
Catalog # :
MA5-17858
Quantity :
300 ug
Price :
US 414.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.2 ug/1x10^6 cells, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent
Species :
Mouse
Clone :
M1/70.15
Isotype :
IgG2b
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
Immunogen :
C57BL/10 spleen cell enriched for T lymphocytes
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.2 ug/1x10^6 cells, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent
Aliases :
antigen CD11b (p170); antigen CD11b (p170); macrophage antigen alpha polypeptide; CD11 antigen-like family member B; CD11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha M; integrin alpha-M; integrin subunit alpha M; integrin, alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; leukocyte integrin alpha-M chain; LOC100351865; Ly-40; MAC1; Mac-1; Mac-1 alpha; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6; unnamed protein product
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA