product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD25 Monoclonal Antibody (PC61.5.3)
catalog :
MA5-17812
quantity :
250 ug
price :
US 242.00
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
PC61.5.3
reactivity :
mouse
application :
immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other
more info or order :
Invitrogen product webpage
citations: 50
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 1b
Kim M, Borcherding N, Ahmed K, Voigt A, Vishwakarma A, Kolb R, et al. CD177 modulates the function and homeostasis of tumor-infiltrating regulatory T cells. Nat Commun. 2021;12:5764 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6a
Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 4d
Rosenbaum M, Gewies A, Pechloff K, Heuser C, Engleitner T, Gehring T, et al. Bcl10-controlled Malt1 paracaspase activity is key for the immune suppressive function of regulatory T cells. Nat Commun. 2019;10:2352 pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig 1a
Chorro L, Suzuki M, Chin S, Williams T, Snapp E, Odagiu L, et al. Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape. Nat Commun. 2018;9:5368 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1h
Gaddis D, Padgett L, Wu R, McSkimming C, Romines V, Taylor A, et al. Apolipoprotein AI prevents regulatory to follicular helper T cell switching during atherosclerosis. Nat Commun. 2018;9:1095 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Lamprianou S, Gysemans C, Bou Saab J, Pontes H, Mathieu C, Meda P. Glibenclamide Prevents Diabetes in NOD Mice. PLoS ONE. 2016;11:e0168839 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Chen S, Miyazaki M, Chandra V, Fisch K, Chang A, Murre C. Id3 Orchestrates Germinal Center B Cell Development. Mol Cell Biol. 2016;36:2543-52 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Goldstein J, Burlion A, Zaragoza B, Sendeyo K, Polansky J, Huehn J, et al. Inhibition of the JAK/STAT Signaling Pathway in Regulatory T Cells Reveals a Very Dynamic Regulation of Foxp3 Expression. PLoS ONE. 2016;11:e0153682 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6s
Haribhai D, Ziegelbauer J, Jia S, Upchurch K, Yan K, Schmitt E, et al. Alternatively Activated Macrophages Boost Induced Regulatory T and Th17 Cell Responses during Immunotherapy for Colitis. J Immunol. 2016;196:3305-17 pubmed publisher
  • immunohistochemistry; mouse; fig 3a
Ziętara N, Łyszkiewicz M, Puchałka J, Witzlau K, Reinhardt A, Förster R, et al. Multicongenic fate mapping quantification of dynamics of thymus colonization. J Exp Med. 2015;212:1589-601 pubmed publisher
  • flow cytometry; mouse
Redpath S, Van Der Werf N, MacDonald A, Maizels R, Taylor M. Schistosoma mansoni Larvae Do Not Expand or Activate Foxp3+ Regulatory T Cells during Their Migratory Phase. Infect Immun. 2015;83:3881-9 pubmed publisher
  • flow cytometry; mouse; fig 5
Redpath S, Van Der Werf N, Cervera A, MacDonald A, Gray D, Maizels R, et al. ICOS controls Foxp3(+) regulatory T-cell expansion, maintenance and IL-10 production during helminth infection. Eur J Immunol. 2013;43:705-15 pubmed publisher
  • flow cytometry; mouse
Yassai M, Cooley B, Gorski J. Developmental dynamics of post-selection thymic DN iNKT. PLoS ONE. 2012;7:e43509 pubmed publisher
  • flow cytometry; mouse; tbl 1
Echeverry A, Saijo S, Schesser K, Adkins B. Yersinia enterocolitica promotes robust mucosal inflammatory T-cell immunity in murine neonates. Infect Immun. 2010;78:3595-608 pubmed publisher
  • flow cytometry; mouse; fig 7
Fahl S, Crittenden R, Allman D, Bender T. c-Myb is required for pro-B cell differentiation. J Immunol. 2009;183:5582-92 pubmed publisher
  • flow cytometry; mouse; fig 3
Robertson S, Messer R, Carmody A, Mittler R, Burlak C, Hasenkrug K. CD137 costimulation of CD8+ T cells confers resistance to suppression by virus-induced regulatory T cells. J Immunol. 2008;180:5267-74 pubmed
  • flow cytometry; mouse
Walsh C, Smith P, Fallon P. Role for CTLA-4 but not CD25+ T cells during Schistosoma mansoni infection of mice. Parasite Immunol. 2007;29:293-308 pubmed
  • flow cytometry; mouse; fig 1
Bros M, Jährling F, Renzing A, Wiechmann N, Dang N, Sutter A, et al. A newly established murine immature dendritic cell line can be differentiated into a mature state, but exerts tolerogenic function upon maturation in the presence of glucocorticoid. Blood. 2007;109:3820-9 pubmed
  • flow cytometry; mouse
Taylor P, Tsoni S, Willment J, Dennehy K, Rosas M, Findon H, et al. Dectin-1 is required for beta-glucan recognition and control of fungal infection. Nat Immunol. 2007;8:31-8 pubmed
  • flow cytometry; mouse; fig 1
Kotner J, Tarleton R. Endogenous CD4(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect Immun. 2007;75:861-9 pubmed
  • flow cytometry; mouse
Cassan C, Piaggio E, Zappulla J, Mars L, Couturier N, Bucciarelli F, et al. Pertussis toxin reduces the number of splenic Foxp3+ regulatory T cells. J Immunol. 2006;177:1552-60 pubmed
  • immunohistochemistry - paraffin section; mouse; fig 5
Leithäuser F, Meinhardt Krajina T, Fink K, Wotschke B, Moller P, Reimann J. Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis. Am J Pathol. 2006;168:1898-909 pubmed
  • flow cytometry; mouse
Sfondrini L, Rossini A, Besusso D, Merlo A, Tagliabue E, Menard S, et al. Antitumor activity of the TLR-5 ligand flagellin in mouse models of cancer. J Immunol. 2006;176:6624-30 pubmed
  • other; mouse
Robertson S, Messer R, Carmody A, Hasenkrug K. In vitro suppression of CD8+ T cell function by Friend virus-induced regulatory T cells. J Immunol. 2006;176:3342-9 pubmed
  • flow cytometry; mouse; fig 1
Lu M, Tayu R, Ikawa T, Masuda K, Matsumoto I, Mugishima H, et al. The earliest thymic progenitors in adults are restricted to T, NK, and dendritic cell lineage and have a potential to form more diverse TCRbeta chains than fetal progenitors. J Immunol. 2005;175:5848-56 pubmed
  • flow cytometry; mouse
Garcia Ojeda M, Dejbakhsh Jones S, Chatterjea Matthes D, Mukhopadhyay A, BitMansour A, Weissman I, et al. Stepwise development of committed progenitors in the bone marrow that generate functional T cells in the absence of the thymus. J Immunol. 2005;175:4363-73 pubmed
  • flow cytometry; mouse
Sangaletti S, Gioiosa L, Guiducci C, Rotta G, Rescigno M, Stoppacciaro A, et al. Accelerated dendritic-cell migration and T-cell priming in SPARC-deficient mice. J Cell Sci. 2005;118:3685-94 pubmed
  • flow cytometry; mouse
Gray P, Arimilli S, Palmer E, Parks G, Alexander Miller M. Altered function in CD8+ T cells following paramyxovirus infection of the respiratory tract. J Virol. 2005;79:3339-49 pubmed
  • flow cytometry; mouse; fig 3
Mangan N, Fallon R, Smith P, Van Rooijen N, McKenzie A, Fallon P. Helminth infection protects mice from anaphylaxis via IL-10-producing B cells. J Immunol. 2004;173:6346-56 pubmed
  • flow cytometry; mouse
Hequet O, Vocanson M, Saint Mezard P, Kaiserlian D, Nicolas J, Berard F. CD4+ T cells prevent skin autoimmunity during chronic autologous graft-versus-host-disease. Am J Transplant. 2004;4:872-8 pubmed
  • flow cytometry; mouse
Wang Y, Lobigs M, Lee E, Mullbacher A. CD8+ T cells mediate recovery and immunopathology in West Nile virus encephalitis. J Virol. 2003;77:13323-34 pubmed
  • flow cytometry; mouse; fig 4
Hogg K, Kumkate S, Mountford A. IL-10 regulates early IL-12-mediated immune responses induced by the radiation-attenuated schistosome vaccine. Int Immunol. 2003;15:1451-9 pubmed
  • flow cytometry; mouse; fig 2
Maris C, Miller J, Altman J, Jacob J. A transgenic mouse model genetically tags all activated CD8 T cells. J Immunol. 2003;171:2393-401 pubmed
  • flow cytometry; mouse; tbl 1
Kawamoto H, Ohmura K, Fujimoto S, Lu M, Ikawa T, Katsura Y. Extensive proliferation of T cell lineage-restricted progenitors in the thymus: an essential process for clonal expression of diverse T cell receptor beta chains. Eur J Immunol. 2003;33:606-15 pubmed
  • flow cytometry; mouse; fig 4
Otten L, Tacchini Cottier F, Lohoff M, Annunziato F, Cosmi L, Scarpellino L, et al. Deregulated MHC class II transactivator expression leads to a strong Th2 bias in CD4+ T lymphocytes. J Immunol. 2003;170:1150-7 pubmed
  • flow cytometry; mouse; fig 8
Saio M, Radoja S, Marino M, Frey A. Tumor-infiltrating macrophages induce apoptosis in activated CD8(+) T cells by a mechanism requiring cell contact and mediated by both the cell-associated form of TNF and nitric oxide. J Immunol. 2001;167:5583-93 pubmed
  • flow cytometry; mouse
Kovalev G, Franklin D, Coffield V, Xiong Y, Su L. An important role of CDK inhibitor p18(INK4c) in modulating antigen receptor-mediated T cell proliferation. J Immunol. 2001;167:3285-92 pubmed
  • flow cytometry; mouse; fig 1
Radoja S, Saio M, Frey A. CD8+ tumor-infiltrating lymphocytes are primed for Fas-mediated activation-induced cell death but are not apoptotic in situ. J Immunol. 2001;166:6074-83 pubmed
  • flow cytometry; mouse; fig 2
Attinger A, MacDonald H, Acha Orbea H. Lymphoid environment limits superantigen and antigen-induced T cell proliferation at high precursor frequency. Eur J Immunol. 2001;31:884-93 pubmed
  • flow cytometry; mouse
Inaba M, Kurasawa K, Mamura M, Kumano K, Saito Y, Iwamoto I. Primed T cells are more resistant to Fas-mediated activation-induced cell death than naive T cells. J Immunol. 1999;163:1315-20 pubmed
  • flow cytometry; mouse
Liu L, Rich B, Inobe J, Chen W, Weiner H. Induction of Th2 cell differentiation in the primary immune response: dendritic cells isolated from adherent cell culture treated with IL-10 prime naive CD4+ T cells to secrete IL-4. Int Immunol. 1998;10:1017-26 pubmed
  • flow cytometry; mouse
van Meerwijk J, Marguerat S, MacDonald H. Homeostasis limits the development of mature CD8+ but not CD4+ thymocytes. J Immunol. 1998;160:2730-4 pubmed
  • flow cytometry; mouse
Aiba Y, Ogawa M. Development of natural killer cells, B lymphocytes, macrophages, and mast cells from single hematopoietic progenitors in culture of murine fetal liver cells. Blood. 1997;90:3923-30 pubmed
  • flow cytometry; mouse; tbl 1
Lin K, Abraham K. Targets of p56(lck) activity in immature thymoblasts: stimulation of the Ras/Raf/MAPK pathway. Int Immunol. 1997;9:291-306 pubmed
  • flow cytometry; mouse; fig 2
Lowenthal J, Zubler R, Nabholz M, MacDonald H. Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes. Nature. 1985;315:669-72 pubmed
  • other; mouse; fig 1
  • immunohistochemistry - frozen section; mouse; fig 3
Ceredig R, Lowenthal J, Nabholz M, MacDonald H. Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells. Nature. 1985;314:98-100 pubmed
Luu K, Patwardhan M, Zeng Q, Wickström S, Lundqvist A, Schwarz H. Regulatory T Cells Inhibit T Cell Activity by Downregulating CD137 Ligand via CD137 Trogocytosis. Cells. 2021;10: pubmed publisher
Permanyer M, Bosnjak B, Glage S, Friedrichsen M, Floess S, Huehn J, et al. Efficient IL-2R signaling differentially affects the stability, function, and composition of the regulatory T-cell pool. Cell Mol Immunol. 2021;18:398-414 pubmed publisher
Webb L, Sengupta S, Edell C, Piedra Quintero Z, Amici S, Miranda J, et al. Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunity. J Clin Invest. 2020;130:1683-1698 pubmed publisher
Ercolini A, Ladle B, Manning E, Pfannenstiel L, Armstrong T, Machiels J, et al. Recruitment of latent pools of high-avidity CD8(+) T cells to the antitumor immune response. J Exp Med. 2005;201:1591-602 pubmed
product information
Product Type :
Antibody
Product Name :
CD25 Monoclonal Antibody (PC61.5.3)
Catalog # :
MA5-17812
Quantity :
250 ug
Price :
US 242.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5-1 ug/1x10^6 cells, Functional Assay: Assay-dependent, Immunoprecipitation: Assay-dependent
Species :
Mouse
Clone :
PC61.5.3
Isotype :
IgG1
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Immunogen :
B6.1 CTL Cell line
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5-1 ug/1x10^6 cells, Functional Assay: Assay-dependent, Immunoprecipitation: Assay-dependent
Aliases :
ADA; ADA1; Adenosine aminohydrolase; adenosine deaminase; CD25; CD25 antigen; cytokine receptor; IDDM10; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; il-2 receptor alpha; IL-2 Receptor alpha chain; IL-2 receptor alpha subunit; IL2 receptor subunit alpha; IL-2 receptor subunit alpha; IL2R; IL-2R alpha; IL-2R alpha chain; IL2R subunit alpha; IL-2R subunit alpha; Il2ra; IL-2RA; IL-2-RA; IL2-RA; Il2ra antibody; IL2RAC; IMD41; interleukin 2 receptor; interleukin 2 receptor alpha chain; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha; interleukin-2 receptor alpha chain; interleukin-2 receptor alpha subunit; interleukin-2 receptor beta chain; Interleukin2 receptor subunit alpha; interleukin-2 receptor subunit alpha; Ly-43; p55; p55 chain; sIL 2R; soluble IL 2 receptor; TAC antigen; TCGFR
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA