CD45 Monoclonal Antibody (YAML501.4) | Invitrogen MA5-17687 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD45 Monoclonal Antibody (YAML501.4)

catalog :

MA5-17687

quantity :

200 ug

price :

US 424.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

YAML501.4

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; loading ...; fig 2b	Smillie C, Biton M, Ordovas Montanes J, Sullivan K, Burgin G, Graham D, et al. Intra- and Inter-cellular Rewiring of the Human Colon during Ulcerative Colitis. Cell. 2019;178:714-730.e22 pubmed publisher
	Kerns J, Belgur C, Kanellias M, Manatakis D, Barrile R, Tien Street W, et al. Safety Profiling of Tumor-targeted T Cell-Bispecific Antibodies with Alveolus Lung- and Colon-on-Chip. Bio Protoc. 2023;13: pubmed publisher
	Moezzi D, Dong Y, Jain R, Lozinski B, Ghorbani S, D Mello C, et al. Expression of antioxidant enzymes in lesions of multiple sclerosis and its models. Sci Rep. 2022;12:12761 pubmed publisher
	Ghorbani S, Jelinek E, Jain R, Buehner B, Li C, Lozinski B, et al. Versican promotes T helper 17 cytotoxic inflammation and impedes oligodendrocyte precursor cell remyelination. Nat Commun. 2022;13:2445 pubmed publisher
	Leveque E, Rouch A, Syrykh C, Mazieres J, Brouchet L, Valitutti S, et al. Phenotypic and Histological Distribution Analysis Identify Mast Cell Heterogeneity in Non-Small Cell Lung Cancer. Cancers (Basel). 2022;14: pubmed publisher
	Lupieri A, Nagata Y, Passos L, Beker Greene D, Kirkwood K, Wylie Sears J, et al. Integration of Functional Imaging, Cytometry, and Unbiased Proteomics Reveals New Features of Endothelial-to-Mesenchymal Transition in Ischemic Mitral Valve Regurgitation in Human Patients. Front Cardiovasc Med. 2021;8:688396 pubmed publisher
	Forsare C, Bendahl P, Moberg E, Levin Tykjær Jørgensen C, Jansson S, Larsson A, et al. Evolution of Estrogen Receptor Status from Primary Tumors to Metastasis and Serially Collected Circulating Tumor Cells. Int J Mol Sci. 2020;21: pubmed publisher
	Li G, Wang M, Caulk A, Cilfone N, Gujja S, Qin L, et al. Chronic mTOR activation induces a degradative smooth muscle cell phenotype. J Clin Invest. 2020;130:1233-1251 pubmed publisher
	Zehender A, Huang J, Györfi A, Matei A, Trinh Minh T, Xu X, et al. The tyrosine phosphatase SHP2 controls TGFβ-induced STAT3 signaling to regulate fibroblast activation and fibrosis. Nat Commun. 2018;9:3259 pubmed publisher
	Awe J, Saranchuk J, Drachenberg D, Mai S. Filtration-based enrichment of circulating tumor cells from all prostate cancer risk groups. Urol Oncol. 2017;35:300-309 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD45 Monoclonal Antibody (YAML501.4)

Catalog # :

MA5-17687

Quantity :

200 ug

Price :

US 424.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Rat

Reactivity :

Human

Applications :

Flow Cytometry: 0.5 ug/1x10^6 cells, Immunocytochemistry: 1:500, Immunohistochemistry (Paraffin): 1:500, Western Blot: Assay-dependent

Species :

Human

Clone :

YAML501.4

Isotype :

IgG2a

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Immunogen :

Rat monoclonal antibody raised against acute myeloid leukemia cells of human origin.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 ug/1x10^6 cells, Immunocytochemistry: 1:500, Immunohistochemistry (Paraffin): 1:500, Western Blot: Assay-dependent

Aliases :

B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

more info or order :

Invitrogen product webpage