CD4 Monoclonal Antibody (W3/25) | Invitrogen MA5-17387 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD4 Monoclonal Antibody (W3/25)

catalog :

MA5-17387

quantity :

500 µg

price :

US 403.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

W3/25

reactivity :

rat

application :

immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments, other

more info or order :

Invitrogen product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
flow cytometry; rat	Qian B, Tonkonogy S, Sartor R. Aberrant innate immune responses in TLR-ligand activated HLA-B27 transgenic rat cells. Inflamm Bowel Dis. 2008;14:1358-65 pubmed publisher
immunohistochemistry; rat	Hou M, Andersson M, Zheng C, Sundblad A, Soder O, Jahnukainen K. Immunomagnetic separation of normal rat testicular cells from Roser's T-cell leukaemia cells is ineffective. Int J Androl. 2009;32:66-73 pubmed
flow cytometry; rat; tbl 1	Hoentjen F, Tonkonogy S, Qian B, Liu B, Dieleman L, Sartor R. CD4(+) T lymphocytes mediate colitis in HLA-B27 transgenic rats monoassociated with nonpathogenic Bacteroides vulgatus. Inflamm Bowel Dis. 2007;13:317-24 pubmed
flow cytometry; rat; tbl 1	Hoentjen F, Tonkonogy S, Liu B, Sartor R, Taurog J, Dieleman L. Adoptive transfer of nontransgenic mesenteric lymph node cells induces colitis in athymic HLA-B27 transgenic nude rats. Clin Exp Immunol. 2006;143:474-83 pubmed
flow cytometry; rat	Qian B, Tonkonogy S, Hoentjen F, Dieleman L, Sartor R. Dysregulated luminal bacterial antigen-specific T-cell responses and antigen-presenting cell function in HLA-B27 transgenic rats with chronic colitis. Immunology. 2005;116:112-21 pubmed
flow cytometry; rat; fig 5	Dieleman L, Hoentjen F, Qian B, Sprengers D, Tjwa E, Torres M, et al. Reduced ratio of protective versus proinflammatory cytokine responses to commensal bacteria in HLA-B27 transgenic rats. Clin Exp Immunol. 2004;136:30-9 pubmed
immunohistochemistry; rat; 1:100	Benedetti S, Bruzzone M, Pollo B, DiMeco F, Magrassi L, Pirola B, et al. Eradication of rat malignant gliomas by retroviral-mediated, in vivo delivery of the interleukin 4 gene. Cancer Res. 1999;59:645-52 pubmed
flow cytometry; rat	Olver S, Goto S, Chiba S, Clouston A, Kelso A. Persistence of donor-reactive CD4+ T cells in liver and spleen of rats tolerant to a liver allograft. Transplantation. 1998;66:132-5 pubmed
immunohistochemistry; rat; 1:100; fig 6	Benedetti S, DiMeco F, Pollo B, Cirenei N, Colombo B, Bruzzone M, et al. Limited efficacy of the HSV-TK/GCV system for gene therapy of malignant gliomas and perspectives for the combined transduction of the interleukin-4 gene. Hum Gene Ther. 1997;8:1345-53 pubmed
other; rat flow cytometry; rat	Williams A, Galfre G, Milstein C. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 1977;12:663-73 pubmed
blocking or activating experiments; rat; fig 1	Webb M, Mason D, Williams A. Inhibition of mixed lymphocyte response by monoclonal antibody specific for a rat T lymphocyte subset. Nature. 1979;282:841-3 pubmed
flow cytometry; rat	White R, Mason D, Williams A, Galfre G, Milstein C. T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody. J Exp Med. 1978;148:664-73 pubmed

product information

Product Type :

Antibody

Product Name :

CD4 Monoclonal Antibody (W3/25)

Catalog # :

MA5-17387

Quantity :

500 µg

Price :

US 403.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Rat

Applications :

Flow Cytometry: 1.0 µg/1x10^6 cells, Functional assay: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent

Species :

Rat

Clone :

W3/25

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

Immunogen :

Rat thymocyte membrane

Format :

Liquid

Applications w/Dilutions :

Aliases :

Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen

more info or order :

Invitrogen product webpage