ICAD Monoclonal Antibody (AF24G5) | Invitrogen MA5-17224 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ICAD Monoclonal Antibody (AF24G5)

catalog :

MA5-17224

quantity :

100 µL

price :

US 402.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AF24G5

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunoprecipitation

product information

Product Type :

Antibody

Product Name :

ICAD Monoclonal Antibody (AF24G5)

Catalog # :

MA5-17224

Quantity :

100 µL

Price :

US 402.00

Clonality :

Monoclonal

Purity :

Ammonium sulfate precipitation

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: Assay-dependent, Immunoprecipitation: 3 µL, Western Blot: 1:2,000

Species :

Human, Mouse, Rat

Clone :

AF24G5

Isotype :

IgG2a

Storage :

-20°C

Description :

ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.

Immunogen :

Recombinant human protein purified from E.coli (His-ICAD)

Format :

liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunoprecipitation: 3 µL, Western Blot: 1:2,000

Aliases :

A330085O09Rik; DFF; DFF1; DFF35; Dff45; DFF-45; Dffa; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; DNA fragmentation factor, alpha subunit; H13; ICAD; ICAD-L; ICAD-S; Inhibitor of CAD; RP23-121D17.3