p16INK4a Monoclonal Antibody (1E12E10) | Invitrogen MA5-17142 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

p16INK4a Monoclonal Antibody (1E12E10)

catalog :

MA5-17142

quantity :

100 uL

price :

US 510.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1E12E10

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; loading ...; fig s2	Safwan Zaiter H, Wagner N, Michiels J, Wagner K. Dynamic Spatiotemporal Expression Pattern of the Senescence-Associated Factor p16Ink4a in Development and Aging. Cells. 2022;11: pubmed publisher
immunohistochemistry; mouse; loading ...; fig 5b	Lee S, Park K, Lee G, Kim S, Song W, Kwon S, et al. Hypoxia-inducible factor-2α mediates senescence-associated intrinsic mechanisms of age-related bone loss. Exp Mol Med. 2021;53:591-604 pubmed publisher
immunohistochemistry - paraffin section; human; 1:10,000; loading ...; fig s7b immunohistochemistry - paraffin section; mouse; 1:10,000; loading ...; fig s6c	Cooper H, Cicalese S, Preston K, Kawai T, Okuno K, Choi E, et al. Targeting mitochondrial fission as a potential therapeutic for abdominal aortic aneurysm. Cardiovasc Res. 2021;117:971-982 pubmed publisher
	Wang J, Lu Y, Carr C, Dhandapani K, Brann D. Senolytic therapy is neuroprotective and improves functional outcome long-term after traumatic brain injury in mice. Front Neurosci. 2023;17:1227705 pubmed publisher
	Raman D, Ch xea ne C, Nicco C, Jeljeli M, Eu J, Cl xe9 ment M, et al. Therapeutic Potential of a Senolytic Approach in a Murine Model of Chronic GVHD. Biology (Basel). 2023;12: pubmed publisher
	Niu X, Xia Y, Luo L, Chen Y, Yuan J, Zhang J, et al. iPSC-sEVs alleviate microglia senescence to protect against ischemic stroke in aged mice. Mater Today Bio. 2023;19:100600 pubmed publisher
	Wu T, Wu Y, Jiang D, Sun W, Zou M, Vasamsetti S, et al. SATB2, coordinated with CUX1, regulates IL-1β-induced senescence-like phenotype in endothelial cells by fine-tuning the atherosclerosis-associated p16INK4a expression. Aging Cell. 2023;22:e13765 pubmed publisher
	Moiseeva V, Cisneros A, Sica V, Deryagin O, Lai Y, Jung S, et al. Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration. Nature. 2023;613:169-178 pubmed publisher
	Jiang D, Sun W, Wu T, Zou M, Vasamsetti S, Zhang X, et al. Post-GWAS functional analysis identifies CUX1 as a regulator of p16INK4a and cellular senescence. Nat Aging. 2022;2:140-154 pubmed publisher
	Yuniartha R, Arfian N, Setyaningsih W, Kencana S, Sari D. Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications. Malays J Med Sci. 2022;29:46-59 pubmed publisher
	Kim H, Jang J, Song M, Kim G, Park C, Lee D, et al. Attenuation of intrinsic ageing of the skin via elimination of senescent dermal fibroblasts with senolytic drugs. J Eur Acad Dermatol Venereol. 2022;36:1125-1135 pubmed publisher
	Rohan T, Ginsberg M, Wang Y, Couch F, Feigelson H, Greenlee R, et al. Molecular markers of risk of subsequent invasive breast cancer in women with ductal carcinoma in situ: protocol for a population-based cohort study. BMJ Open. 2021;11:e053397 pubmed publisher
	Lian W, Wu R, Chen Y, Ko J, Wang S, Jahr H, et al. MicroRNA-29a Mitigates Osteoblast Senescence and Counteracts Bone Loss through Oxidation Resistance-1 Control of FoxO3 Methylation. Antioxidants (Basel). 2021;10: pubmed publisher
	Chaney K, Perrino M, Kershner L, Patel A, Wu J, Choi K, et al. Cdkn2a Loss in a Model of Neurofibroma Demonstrates Stepwise Tumor Progression to Atypical Neurofibroma and MPNST. Cancer Res. 2020;80:4720-4730 pubmed publisher
	Polonis K, Becari C, Chahal C, Zhang Y, Allen A, Kellogg T, et al. Chronic Intermittent Hypoxia Triggers a Senescence-like Phenotype in Human White Preadipocytes. Sci Rep. 2020;10:6846 pubmed publisher
	Chen H, Chen H, Liang J, Gu X, Zhou J, Xie C, et al. TGF-β1/IL-11/MEK/ERK signaling mediates senescence-associated pulmonary fibrosis in a stress-induced premature senescence model of Bmi-1 deficiency. Exp Mol Med. 2020;52:130-151 pubmed publisher

product information

Product Type :

Antibody

Product Name :

p16INK4a Monoclonal Antibody (1E12E10)

Catalog # :

MA5-17142

Quantity :

100 uL

Price :

US 510.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 1:10,000, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Human, Mouse, Rat

Clone :

1E12E10

Isotype :

IgG1

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

Immunogen :

Purified recombinant fragment of P16 expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

Alternative reading frame; Arf; ARF-INK4a; CDK4 inhibitor p16 INK4; CDK4 inhibitor p16-INK4; CDK4I; CDKN2; Cdkn2a; cdkn2a {ECO:0000312; cell cycle inhibitor; cell cycle negative regulator beta; cell cycle regulator; CMM2; Cyclin dependent kinase 4 inhibitor A; cyclin dependent kinase inhibitor 2A; Cyclin dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2a p16Ink4a; cyclin-dependent kinase inhibitor 2a p19Arf; cyclin-dependent kinase inhibitor 2A, isoform 1; cyclin-dependent kinase inhibitor 2A, isoform 2; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; EMBL:AAL76336.1, ECO:0000312; EMBL:AAL76338.1, ECO:0000312; HGNC:1787; inhibits CDK4; INK4; INK4A; Ink4a/Arf; INK4a-ARF; isoforms 1/2/3; Melanoma p16 inhibits CDK4; mitochondrial smARF; MLM; MTS1; MTS-1; Multiple tumor suppressor 1; P14; P14 P16; P14ARF; p16; p16(INK4a); p16Cdkn2a; P16INK4; p16-INK4; p16INK4a; p16-INK4a; P19; P19 TP16; p19 ; P19ARF; Pctr1; RGD:2323}; TP16; Tumor suppressor ARF

more info or order :

Invitrogen product webpage