iNOS Monoclonal Antibody (4E5) | Invitrogen MA5-17139 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

iNOS Monoclonal Antibody (4E5)

catalog :

MA5-17139

quantity :

100 ug

price :

US 546.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4.00E+05

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; loading ...; fig 4a	Tan H, Song Y, Chen J, Zhang N, Wang Q, Li Q, et al. Platelet-Like Fusogenic Liposome-Mediated Targeting Delivery of miR-21 Improves Myocardial Remodeling by Reprogramming Macrophages Post Myocardial Ischemia-Reperfusion Injury. Adv Sci (Weinh). 2021;8:e2100787 pubmed publisher
	Chen P, Guo Z, Lei J, Wang Y. Pomegranate polyphenol punicalin ameliorates lipopolysaccharide-induced memory impairment, behavioral disorders, oxidative stress, and neuroinflammation via inhibition of TLR4-NF-кB pathway. Phytother Res. 2024;38:3489-3508 pubmed publisher
	Liu X, Li H, Feng Y, Guo H, Li Y, Ke J, et al. Resatorvid alleviates experimental inflammatory TMJOA by restraining chondrocyte pyroptosis and synovial inflammation. Arthritis Res Ther. 2023;25:230 pubmed publisher
	Li Y, Zhang C, Zhao Z. CircSLCO3A1 depletion ameliorates lipopolysaccharide-induced inflammation and apoptosis of human pulmonary alveolar epithelial cells through the miR-424-5p/HMGB3 pathway. Mol Cell Toxicol. 2023;:1-12 pubmed publisher
	Gu C, Chen M, Li X, Geng D, Wang C. MAGL regulates synovial macrophage polarization vis inhibition of mitophagy in osteoarthritic pain. Mol Med Rep. 2023;27: pubmed publisher
	Rivas Santisteban R, Ra xef ch I, Aguinaga D, Saura C, Franco R, Navarro G. The Expression of Cellular Prion Protein, PrPC, Favors pTau Propagation and Blocks NMDAR Signaling in Primary Cortical Neurons. Cells. 2023;12: pubmed publisher
	Roy T, Banang Mbeumi S, Boateng S, Ruiz E, Chamcheu R, Kang L, et al. Dual targeting of mTOR/IL-17A and autophagy by fisetin alleviates psoriasis-like skin inflammation. Front Immunol. 2022;13:1075804 pubmed publisher
	Ismail O, Rashed N. Riboflavin attenuates tartrazine toxicity in the cerebellar cortex of adult albino rat. Sci Rep. 2022;12:19346 pubmed publisher
	Wu L, Xu Y, Zhao H, Zhou Y, Chen Y, Yang S, et al. FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape. Theranostics. 2022;12:842-858 pubmed publisher
	Fayzullin A, Churbanov S, Ignatieva N, Zakharkina O, Tokarev M, Mudryak D, et al. Local Delivery of Pirfenidone by PLA Implants Modifies Foreign Body Reaction and Prevents Fibrosis. Biomedicines. 2021;9: pubmed publisher
	Dutta B, Goswami R, Rahaman S. TRPV4 Plays a Role in Matrix Stiffness-Induced Macrophage Polarization. Front Immunol. 2020;11:570195 pubmed publisher
	Wu L, Zhang X, Zheng L, Zhao H, Yan G, Zhang Q, et al. RIPK3 Orchestrates Fatty Acid Metabolism in Tumor-Associated Macrophages and Hepatocarcinogenesis. Cancer Immunol Res. 2020;8:710-721 pubmed publisher
	Sadler R, Cramer J, Heindl S, Kostidis S, Betz D, Zuurbier K, et al. Short-Chain Fatty Acids Improve Poststroke Recovery via Immunological Mechanisms. J Neurosci. 2020;40:1162-1173 pubmed publisher
	Huang M, Wu R, Chen L, Peng Q, Li S, Zhang Y, et al. S100A9 Regulates MDSCs-Mediated Immune Suppression via the RAGE and TLR4 Signaling Pathways in Colorectal Carcinoma. Front Immunol. 2019;10:2243 pubmed publisher
	Yan G, Zhao H, Zhang Q, Zhou Y, Wu L, Lei J, et al. A RIPK3-PGE2 circuit mediates myeloid-derived suppressor cell-potentiated colorectal carcinogenesis. Cancer Res. 2018;: pubmed publisher
	Garrido P, Shalaby A, WALSH E, Keane N, Webber M, Keane M, et al. Impact of inducible nitric oxide synthase (iNOS) expression on triple negative breast cancer outcome and activation of EGFR and ERK signaling pathways. Oncotarget. 2017;8:80568-80588 pubmed publisher
	Zhang M, He Y, Sun X, Li Q, Wang W, Zhao A, et al. A high M1/M2 ratio of tumor-associated macrophages is associated with extended survival in ovarian cancer patients. J Ovarian Res. 2014;7:19 pubmed publisher

product information

Product Type :

Antibody

Product Name :

iNOS Monoclonal Antibody (4E5)

Catalog # :

MA5-17139

Quantity :

100 ug

Price :

US 546.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ELISA: 1:10,000, Flow Cytometry: 1:200-1:400, Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Human, Mouse

Clone :

4.00E+05

Isotype :

IgG1

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

Immunogen :

Purified recombinant fragment of human NOS2 expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Flow Cytometry: 1:200-1:400, Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

Hepatocyte NOS; hepatocytes; HEP-NOS; inducible nitric oxide synthase; inducible NO synthase; Inducible NOS; iNos; i-NOS; Inosl; MAC-NOS; macrophage NOS; nitric oxide synthase 2; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS; NOS type II; NOS, type II; Nos2; Nos-2; Nos2a; NOS-II; OTTMUSP00000000202; peptidyl-cysteine S-nitrosylase NOS2

more info or order :

Invitrogen product webpage