CD163 Monoclonal Antibody (ED2) | Invitrogen MA5-16658 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD163 Monoclonal Antibody (ED2)

catalog :

MA5-16658

quantity :

250 ug

price :

US 612.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ED2

reactivity :

rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
flow cytometry; rat; loading ...; fig 3j	Liu M, Yin L, Li W, Hu J, Wang H, Ye B, et al. C1q/TNF-related protein-9 promotes macrophage polarization and improves cardiac dysfunction after myocardial infarction. J Cell Physiol. 2019;234:18731-18747 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:100; loading ...; fig 9d	Pridans C, Raper A, Davis G, Alves J, Sauter K, Lefèvre L, et al. Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus. J Immunol. 2018;201:2683-2699 pubmed publisher
immunohistochemistry; rat	Benedetti S, Bruzzone M, Pollo B, DiMeco F, Magrassi L, Pirola B, et al. Eradication of rat malignant gliomas by retroviral-mediated, in vivo delivery of the interleukin 4 gene. Cancer Res. 1999;59:645-52 pubmed
immunohistochemistry; rat; 1:250; fig 6	Benedetti S, DiMeco F, Pollo B, Cirenei N, Colombo B, Bruzzone M, et al. Limited efficacy of the HSV-TK/GCV system for gene therapy of malignant gliomas and perspectives for the combined transduction of the interleukin-4 gene. Hum Gene Ther. 1997;8:1345-53 pubmed

product information

Product Type :

Antibody

Product Name :

CD163 Monoclonal Antibody (ED2)

Catalog # :

MA5-16658

Quantity :

250 ug

Price :

US 612.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Rat

Applications :

Flow Cytometry: Assay-dependent, Immunocytochemistry: Assay-dependent, Immunohistochemistry (Frozen): 1:50-1:100, Immunohistochemistry (Paraffin): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Species :

Rat

Clone :

ED2

Isotype :

IgG1

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

Immunogen :

Rat Spleen cell homogenate

Format :

Liquid

Applications w/Dilutions :

Aliases :

CD163; CD163 antigen; CD163 molecule; CD163v2; CD163v3; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; putative CD163 antigen; SCARI1; Scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163; Soluble sCD163

more info or order :

Invitrogen product webpage