F4/80 Monoclonal Antibody (Cl:A3-1) | Invitrogen MA5-16624 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

F4/80 Monoclonal Antibody (Cl:A3-1)

catalog :

MA5-16624

quantity :

2 mL

price :

US 582.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

Cl:A3-1

reactivity :

mouse

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 5 ug/ml; loading ...; fig 2a	Wu X, Shu L, Zhang Z, Li J, Zong J, Cheong L, et al. Adipocyte Fatty Acid Binding Protein Promotes the Onset and Progression of Liver Fibrosis via Mediating the Crosstalk between Liver Sinusoidal Endothelial Cells and Hepatic Stellate Cells. Adv Sci (Weinh). 2021;8:e2003721 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig s11	Nelson C, Hakim C, Ousterout D, Thakore P, Moreb E, Castellanos Rivera R, et al. In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy. Science. 2016;351:403-7 pubmed publisher
flow cytometry; mouse	Hufford M, Kim T, Sun J, Braciale T. Antiviral CD8+ T cell effector activities in situ are regulated by target cell type. J Exp Med. 2011;208:167-80 pubmed publisher
flow cytometry; mouse; tbl 1	Thompson J, Chu Y, Glass J, Tapp A, Brown S. The manganese superoxide dismutase mimetic, M40403, protects adult mice from lethal total body irradiation. Free Radic Res. 2010;44:529-40 pubmed publisher
flow cytometry; mouse	Thompson J, Chu Y, Glass J, Brown S. Absence of IL-23p19 in donor allogeneic cells reduces mortality from acute GVHD. Bone Marrow Transplant. 2010;45:712-22 pubmed publisher
flow cytometry; mouse; fig 1	Dewals B, Hoving J, Leeto M, Marillier R, Govender U, Cutler A, et al. IL-4Ralpha responsiveness of non-CD4 T cells contributes to resistance in schistosoma mansoni infection in pan-T cell-specific IL-4Ralpha-deficient mice. Am J Pathol. 2009;175:706-16 pubmed publisher
flow cytometry; mouse; fig 2	Serada S, Fujimoto M, Mihara M, Koike N, Ohsugi Y, Nomura S, et al. IL-6 blockade inhibits the induction of myelin antigen-specific Th17 cells and Th1 cells in experimental autoimmune encephalomyelitis. Proc Natl Acad Sci U S A. 2008;105:9041-6 pubmed publisher
flow cytometry; mouse; fig 1	Culshaw S, Millington O, Brewer J, McInnes I. Murine neutrophils present Class II restricted antigen. Immunol Lett. 2008;118:49-54 pubmed publisher
	Li J, Ruggiero Ruff R, He Y, Qiu X, Lainez N, Villa P, et al. Sexual dimorphism in obesity is governed by RELMα regulation of adipose macrophages and eosinophils. elife. 2023;12: pubmed publisher
	Porto B, Litvack M, Cen Y, Lok I, Bouch S, Norris M, et al. Alveolar-like Macrophages Attenuate Respiratory Syncytial Virus Infection. Viruses. 2021;13: pubmed publisher
	Bruno G, De Logu F, Souza Monteiro de Araújo D, Subbiani A, Lunardi F, Rettori S, et al. β2-and β3-Adrenergic Receptors Contribute to Cancer-Evoked Pain in a Mouse Model of Osteosarcoma via Modulation of Neural Macrophages. Front Pharmacol. 2021;12:697912 pubmed publisher
	Souza Monteiro de Araújo D, De Logu F, Adembri C, Rizzo S, Janal M, Landini L, et al. TRPA1 mediates damage of the retina induced by ischemia and reperfusion in mice. Cell Death Dis. 2020;11:633 pubmed publisher
	De Logu F, De Prá S, De David Antoniazzi C, Kudsi S, Ferro P, Landini L, et al. Macrophages and Schwann cell TRPA1 mediate chronic allodynia in a mouse model of complex regional pain syndrome type I. Brain Behav Immun. 2020;88:535-546 pubmed publisher
	Fultz R, Engevik M, Shi Z, Hall A, Herrmann B, Ganesh B, et al. Phagocytosis by macrophages depends on histamine H2 receptor signaling and scavenger receptor 1. Microbiologyopen. 2019;8:e908 pubmed publisher
	Wang H, Feng Y, Jin X, Xia R, Cheng Y, Liu X, et al. Augmentation of hypoxia-inducible factor-1-alpha in reinfused blood cells enhances diabetic ischemic wound closure in mice. Oncotarget. 2017;8:114251-114258 pubmed publisher
	De Logu F, Nassini R, Materazzi S, Carvalho Gonçalves M, Nosi D, Rossi Degl Innocenti D, et al. Schwann cell TRPA1 mediates neuroinflammation that sustains macrophage-dependent neuropathic pain in mice. Nat Commun. 2017;8:1887 pubmed publisher

product information

Product Type :

Antibody

Product Name :

F4/80 Monoclonal Antibody (Cl:A3-1)

Catalog # :

MA5-16624

Quantity :

2 mL

Price :

US 582.00

Clonality :

Monoclonal

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: Neat-1:10, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): Assay-dependent, Immunohistochemistry: Assay-dependent, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent, Radioimmune assays: Assay-dependent

Species :

Mouse

Clone :

Cl:A3-1

Isotype :

IgG2b

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Immunogen :

Thioglycollate stimulated peritoneal macrophages from C57/BL mice.

Format :

Liquid

Applications w/Dilutions :

Aliases :

ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3

more info or order :

Invitrogen product webpage