CD19 Monoclonal Antibody (6D5) | Invitrogen MA5-16534 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD19 Monoclonal Antibody (6D5)

catalog :

MA5-16534

quantity :

250 ug

price :

US 438.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

6D5

reactivity :

mouse

application :

immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 35

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...	Panda S, Wigerblad G, Jiang L, Jiménez Andrade Y, Iyer V, Shen Y, et al. IL-4 controls activated neutrophil FcγR2b expression and migration into inflamed joints. Proc Natl Acad Sci U S A. 2020;117:3103-3113 pubmed publisher
flow cytometry; mouse; loading ...; fig s2a	Gubernatorova E, Gorshkova E, Namakanova O, Zvartsev R, Hidalgo J, Drutskaya M, et al. Non-redundant Functions of IL-6 Produced by Macrophages and Dendritic Cells in Allergic Airway Inflammation. Front Immunol. 2018;9:2718 pubmed publisher
flow cytometry; mouse; loading ...; fig s3a	Sharma D, Malik A, Guy C, Vogel P, Kanneganti T. TNF/TNFR axis promotes pyrin inflammasome activation and distinctly modulates pyrin inflammasomopathy. J Clin Invest. 2019;129:150-162 pubmed publisher
flow cytometry; mouse; loading ...; fig 2	Lin Y, Wang L, Lee C, Chen S. Flt3 ligand treatment reduces enterovirus A71 lethality in mice with enhanced B cell responses. Sci Rep. 2018;8:12184 pubmed publisher
flow cytometry; mouse; loading ...; fig s2a	Ferdinand J, Richard A, Meylan F, Al Shamkhani A, Siegel R. Cleavage of TL1A Differentially Regulates Its Effects on Innate and Adaptive Immune Cells. J Immunol. 2018;200:1360-1369 pubmed publisher
flow cytometry; mouse; 1:400; loading ...	Mao A, Ishizuka I, Kasal D, Mandal M, Bendelac A. A shared Runx1-bound Zbtb16 enhancer directs innate and innate-like lymphoid lineage development. Nat Commun. 2017;8:863 pubmed publisher
blocking or activating experiments; mouse; loading ...; fig 1	Nowyhed H, Chandra S, Kiosses W, Marcovecchio P, Andary F, Zhao M, et al. ATP Binding Cassette Transporter ABCA7 Regulates NKT Cell Development and Function by Controlling CD1d Expression and Lipid Raft Content. Sci Rep. 2017;7:40273 pubmed publisher
flow cytometry; mouse; loading ...; fig s1	Park K, Mikulski Z, Seo G, Andreyev A, Marcovecchio P, Blatchley A, et al. The transcription factor NR4A3 controls CD103+ dendritic cell migration. J Clin Invest. 2016;126:4603-4615 pubmed publisher
flow cytometry; mouse; fig 1	Sin S, Kim Y, Eason A, Dittmer D. KSHV Latency Locus Cooperates with Myc to Drive Lymphoma in Mice. PLoS Pathog. 2015;11:e1005135 pubmed publisher
flow cytometry; mouse	Amado I, Berges J, Luther R, Mailhé M, Garcia S, Bandeira A, et al. IL-2 coordinates IL-2-producing and regulatory T cell interplay. J Exp Med. 2013;210:2707-20 pubmed publisher
flow cytometry; mouse	Mandal M, Donnelly R, Elkabes S, Zhang P, Davini D, David B, et al. Maternal immune stimulation during pregnancy shapes the immunological phenotype of offspring. Brain Behav Immun. 2013;33:33-45 pubmed publisher
flow cytometry; mouse; fig 3	Mandal M, Marzouk A, Donnelly R, Ponzio N. Maternal immune stimulation during pregnancy affects adaptive immunity in offspring to promote development of TH17 cells. Brain Behav Immun. 2011;25:863-71 pubmed publisher
flow cytometry; mouse	Horman S, Velu C, Chaubey A, Bourdeau T, Zhu J, Paul W, et al. Gfi1 integrates progenitor versus granulocytic transcriptional programming. Blood. 2009;113:5466-75 pubmed publisher
flow cytometry; mouse; fig 10	Kanwar N, Fayyazi A, Backofen B, Nitsche M, Dressel R, von Mollard G. Thymic alterations in mice deficient for the SNARE protein VAMP8/endobrevin. Cell Tissue Res. 2008;334:227-42 pubmed publisher
flow cytometry; mouse	Walsh C, Smith P, Fallon P. Role for CTLA-4 but not CD25+ T cells during Schistosoma mansoni infection of mice. Parasite Immunol. 2007;29:293-308 pubmed
flow cytometry; mouse	Fallon P, Ballantyne S, Mangan N, Barlow J, Dasvarma A, Hewett D, et al. Identification of an interleukin (IL)-25-dependent cell population that provides IL-4, IL-5, and IL-13 at the onset of helminth expulsion. J Exp Med. 2006;203:1105-16 pubmed
flow cytometry; mouse; fig 10	Mangan N, Van Rooijen N, McKenzie A, Fallon P. Helminth-modified pulmonary immune response protects mice from allergen-induced airway hyperresponsiveness. J Immunol. 2006;176:138-47 pubmed
flow cytometry; mouse; fig 6	Grisaru D, Pick M, Perry C, Sklan E, Almog R, Goldberg I, et al. Hydrolytic and nonenzymatic functions of acetylcholinesterase comodulate hemopoietic stress responses. J Immunol. 2006;176:27-35 pubmed
flow cytometry; mouse	Iwasaki H, Mizuno S, Mayfield R, Shigematsu H, Arinobu Y, Seed B, et al. Identification of eosinophil lineage-committed progenitors in the murine bone marrow. J Exp Med. 2005;201:1891-7 pubmed
flow cytometry; mouse; fig 1	Iwasaki H, Somoza C, Shigematsu H, Duprez E, Iwasaki Arai J, Mizuno S, et al. Distinctive and indispensable roles of PU.1 in maintenance of hematopoietic stem cells and their differentiation. Blood. 2005;106:1590-600 pubmed
flow cytometry; mouse	Growney J, Shigematsu H, Li Z, Lee B, Adelsperger J, Rowan R, et al. Loss of Runx1 perturbs adult hematopoiesis and is associated with a myeloproliferative phenotype. Blood. 2005;106:494-504 pubmed
flow cytometry; mouse; fig 7	Mangan N, Fallon R, Smith P, Van Rooijen N, McKenzie A, Fallon P. Helminth infection protects mice from anaphylaxis via IL-10-producing B cells. J Immunol. 2004;173:6346-56 pubmed
flow cytometry; mouse	Smith P, Walsh C, Mangan N, Fallon R, Sayers J, McKenzie A, et al. Schistosoma mansoni worms induce anergy of T cells via selective up-regulation of programmed death ligand 1 on macrophages. J Immunol. 2004;173:1240-8 pubmed
	Carpentier K, Davenport B, HAIST K, McCarthy M, May N, Robison A, et al. Discrete viral E2 lysine residues and scavenger receptor MARCO are required for clearance of circulating alphaviruses. elife. 2019;8: pubmed publisher
	Janela B, Patel A, Lau M, Goh C, Msallam R, Kong W, et al. A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils. Immunity. 2019;50:1069-1083.e8 pubmed publisher
	Moschovakis G, Bubke A, Friedrichsen M, Ristenpart J, Back J, Falk C, et al. The chemokine receptor CCR7 is a promising target for rheumatoid arthritis therapy. Cell Mol Immunol. 2018;: pubmed publisher
	Pyle C, Uwadiae F, Swieboda D, Harker J. Early IL-6 signalling promotes IL-27 dependent maturation of regulatory T cells in the lungs and resolution of viral immunopathology. PLoS Pathog. 2017;13:e1006640 pubmed publisher
	Karki R, Man S, Malireddi R, Kesavardhana S, Zhu Q, Burton A, et al. NLRC3 is an inhibitory sensor of PI3K-mTOR pathways in cancer. Nature. 2016;540:583-587 pubmed publisher
	Sochalska M, Schuler F, Weiss J, Prchal Murphy M, Sexl V, Villunger A. MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis. Oncogene. 2017;36:2066-2073 pubmed publisher
	Liu X, Jiang X, Liu R, Wang L, Qian T, Zheng Y, et al. B cells expressing CD11b effectively inhibit CD4+ T-cell responses and ameliorate experimental autoimmune hepatitis in mice. Hepatology. 2015;62:1563-75 pubmed publisher
	Leonhardt F, Grundmann S, Behe M, Bluhm F, Dumont R, Braun F, et al. Inflammatory neovascularization during graft-versus-host disease is regulated by ?v integrin and miR-100. Blood. 2013;121:3307-18 pubmed publisher
	Besseyrias V, Fiorini E, Strobl L, Zimber Strobl U, Dumortier A, Koch U, et al. Hierarchy of Notch-Delta interactions promoting T cell lineage commitment and maturation. J Exp Med. 2007;204:331-43 pubmed
	Iwasaki Arai J, Iwasaki H, Miyamoto T, Watanabe S, Akashi K. Enforced granulocyte/macrophage colony-stimulating factor signals do not support lymphopoiesis, but instruct lymphoid to myelomonocytic lineage conversion. J Exp Med. 2003;197:1311-22 pubmed
	Goodyear C, Silverman G. Death by a B cell superantigen: In vivo VH-targeted apoptotic supraclonal B cell deletion by a Staphylococcal Toxin. J Exp Med. 2003;197:1125-39 pubmed
	Li Y, Li H, Weigert M. Autoreactive B cells in the marginal zone that express dual receptors. J Exp Med. 2002;195:181-8 pubmed

product information

Product Type :

Antibody

Product Name :

CD19 Monoclonal Antibody (6D5)

Catalog # :

MA5-16534

Quantity :

250 ug

Price :

US 438.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 1:10-1:50, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent

Species :

Mouse

Clone :

6D5

Isotype :

IgG2a

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

Immunogen :

Human K562 cell line transfected with murine CD19

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:10-1:50, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: Assay-dependent

Aliases :

AW495831; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Cd19; CD19 antigen; CD19 molecule; CVID3; differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12

more info or order :

Invitrogen product webpage