product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Vimentin Monoclonal Antibody (SP20)
catalog :
MA5-16409
quantity :
500 uL
price :
US 562.00
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SP20
reactivity :
African green monkey, human, mouse, domestic sheep
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, other
more info or order :
citations: 46
Published Application/Species/Sample/DilutionReference
  • other; human; loading ...; fig 1,3
Jafari A, Qanie D, Andersen T, Zhang Y, Chen L, Postert B, et al. Legumain Regulates Differentiation Fate of Human Bone Marrow Stromal Cells and Is Altered in Postmenopausal Osteoporosis. Stem Cell Reports. 2017;8:373-386 pubmed publisher
  • immunohistochemistry - paraffin section; domestic sheep; loading ...; fig 4
Kjærgaard K, Dreyer C, Ditzel N, Andreasen C, Chen L, Sheikh S, et al. Bone Formation by Sheep Stem Cells in an Ectopic Mouse Model: Comparison of Adipose and Bone Marrow Derived Cells and Identification of Donor-Derived Bone by Antibody Staining. Stem Cells Int. 2016;2016:3846971 pubmed
  • immunohistochemistry - paraffin section; human; loading ...; fig 4b
Bhagirath D, Zhao X, Mirza S, West W, Band H, Band V. Mutant PIK3CA Induces EMT in a Cell Type Specific Manner. PLoS ONE. 2016;11:e0167064 pubmed publisher
  • immunohistochemistry; human; fig 4j
Harkness L, Zaher W, Ditzel N, Isa A, Kassem M. CD146/MCAM defines functionality of human bone marrow stromal stem cell populations. Stem Cell Res Ther. 2016;7:4 pubmed publisher
  • western blot; human; fig s3
Bhagirath D, Zhao X, West W, Qiu F, Band H, Band V. Cell type of origin as well as genetic alterations contribute to breast cancer phenotypes. Oncotarget. 2015;6:9018-30 pubmed
  • immunohistochemistry - paraffin section; human
Chen Q, Gu Y, Liu B. Clinicopathological characteristics of kidney mucinous tubular and spindle cell carcinoma. Int J Clin Exp Pathol. 2015;8:1007-12 pubmed
  • immunohistochemistry; human; 1:50
Calangiu C, Simionescu C, Stepan A, Cernea D, Zăvoi R, Mărgăritescu C. The expression of CK19, vimentin and E-cadherin in differentiated thyroid carcinomas. Rom J Morphol Embryol. 2014;55:919-25 pubmed
  • immunohistochemistry - paraffin section; human
Moser B, Megerle A, Bekos C, Janik S, Szerafin T, Birner P, et al. Local and systemic RAGE axis changes in pulmonary hypertension: CTEPH and iPAH. PLoS ONE. 2014;9:e106440 pubmed publisher
  • immunocytochemistry; human
Treloar K, Simpson M, Haridas P, Manton K, Leavesley D, McElwain D, et al. Multiple types of data are required to identify the mechanisms influencing the spatial expansion of melanoma cell colonies. BMC Syst Biol. 2013;7:137 pubmed publisher
  • immunohistochemistry; human; 1:300
Gremel G, Bergman J, Djureinovic D, Edqvist P, Maindad V, Bharambe B, et al. A systematic analysis of commonly used antibodies in cancer diagnostics. Histopathology. 2014;64:293-305 pubmed publisher
  • immunohistochemistry; mouse
Burden Gulley S, Zhou Z, Craig S, Lu Z, Brady Kalnay S. Molecular Magnetic Resonance Imaging of Tumors with a PTPµ Targeted Contrast Agent. Transl Oncol. 2013;6:329-37 pubmed
  • immunocytochemistry; human
Mace T, Ameen Z, Collins A, Wojcik S, Mair M, Young G, et al. Pancreatic cancer-associated stellate cells promote differentiation of myeloid-derived suppressor cells in a STAT3-dependent manner. Cancer Res. 2013;73:3007-18 pubmed publisher
  • immunohistochemistry; human; 1:300
Schoppmann S, Jesch B, Riegler M, Maroske F, Schwameis K, Jomrich G, et al. Podoplanin expressing cancer associated fibroblasts are associated with unfavourable prognosis in adenocarcinoma of the esophagus. Clin Exp Metastasis. 2013;30:441-6 pubmed publisher
  • immunohistochemistry; mouse; 1:200
Hegde G, de la Cruz C, Eastham Anderson J, Zheng Y, Sweet Cordero E, Jackson E. Residual tumor cells that drive disease relapse after chemotherapy do not have enhanced tumor initiating capacity. PLoS ONE. 2012;7:e45647 pubmed publisher
  • immunohistochemistry; human
Majumdar K, Tyagi I, Saran R, Sakhuja P, Sharma A. Medulloblastoma with focal divergent/teratoid differentiation. Brain Tumor Pathol. 2013;30:50-6 pubmed publisher
  • immunohistochemistry; human; 1:100
Pavlakis K, Vrekoussis T, Messini I, Voulgaris Z, Chrysanthakis D, Yiannou P, et al. Mucinous epithelial lesions in endometrial curettage material: a diagnostic challenge. Appl Immunohistochem Mol Morphol. 2012;20:607-13 pubmed publisher
  • immunohistochemistry; African green monkey
Irizarry Rovira A, Lynch S, David M, Ramos Vara J. Gonadoblastoma in the ovaries of a lesser galago (Galago senegalensis braccatus). J Comp Pathol. 2012;147:204-8 pubmed publisher
  • western blot; human; fig 3
Zhao X, Malhotra G, Band H, Band V. Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells. PLoS ONE. 2012;7:e35338 pubmed publisher
  • immunohistochemistry; human
Kaur H, Burden Gulley S, Phillips Mason P, Basilion J, Sloan A, Brady Kalnay S. Protein tyrosine phosphatase mu regulates glioblastoma cell growth and survival in vivo. Neuro Oncol. 2012;14:561-73 pubmed publisher
  • immunohistochemistry; human; 1:300
Yin W, Li J, Chan J. Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review. Diagn Pathol. 2012;7:39 pubmed publisher
  • immunohistochemistry; human; 1:200
Haug K, Azqueta A, Johnsen Soriano S, Shahdadfar A, Drolsum L, Moe M, et al. Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan. Acta Ophthalmol. 2013;91:219-25 pubmed publisher
  • immunocytochemistry; human
Lupu Meiri M, Geras Raaka E, Lupu R, Shapira H, Sandbank J, Segal L, et al. Knock-down of plasminogen-activator inhibitor-1 enhances expression of E-cadherin and promotes epithelial differentiation of human pancreatic adenocarcinoma cells. J Cell Physiol. 2012;227:3621-8 pubmed publisher
  • immunohistochemistry; African green monkey
Durkes A, Garner M, Juan Sallés C, Ramos Vara J. Immunohistochemical characterization of nonhuman primate ovarian sex cord-stromal tumors. Vet Pathol. 2012;49:834-8 pubmed publisher
  • immunohistochemistry; human
Kaur H, Phillips Mason P, Burden Gulley S, Kerstetter Fogle A, Basilion J, Sloan A, et al. Cadherin-11, a marker of the mesenchymal phenotype, regulates glioblastoma cell migration and survival in vivo. Mol Cancer Res. 2012;10:293-304 pubmed publisher
  • immunohistochemistry; human
Ersoz S, Turgutalp H, Mungan S, Güvendı G, Guven S. Cotyledonoid leiomyoma of uterus: a case report. Turk Patoloji Derg. 2011;27:257-60 pubmed publisher
  • immunocytochemistry; human
  • immunohistochemistry; human
  • western blot; human
Rojas A, Liu G, Coleman I, Nelson P, Zhang M, Dash R, et al. IL-6 promotes prostate tumorigenesis and progression through autocrine cross-activation of IGF-IR. Oncogene. 2011;30:2345-55 pubmed publisher
  • immunohistochemistry; human; 1:100
Yu G, Qu G, Kong L, Pan X, Wang W, Lv J. Primary myoepithelial carcinoma of the larynx: case report and review of the literature. Pathol Res Pract. 2011;207:127-30 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; tbl 2
  • immunohistochemistry; human; 1:100
Eisenthal A, Trejo L, Shtabsky A, Bedny F, Brazowski E. A novel assessment of the quality of immunohistostaining overcomes the limitations of current methods. Pathol Res Pract. 2008;204:323-8 pubmed publisher
  • immunohistochemistry; human
Liu H, Fan Q, Zhang Z, Li X, Yu H, Meng F. Basal-HER2 phenotype shows poorer survival than basal-like phenotype in hormone receptor-negative invasive breast cancers. Hum Pathol. 2008;39:167-74 pubmed
  • immunohistochemistry; human
Dilbaz B, Bayoglu Y, Oral S, Cavusoglu D, Uluoglu O, Dilbaz S. Laparoscopic resection of urinary bladder paraganglioma: a case report. Surg Laparosc Endosc Percutan Tech. 2006;16:58-61 pubmed
  • immunohistochemistry; mouse; 1:75
Valkova N, Yunis R, Mak S, Kang K, Kültz D. Nek8 mutation causes overexpression of galectin-1, sorcin, and vimentin and accumulation of the major urinary protein in renal cysts of jck mice. Mol Cell Proteomics. 2005;4:1009-18 pubmed
Kamens J, Nance S, Koss C, Xu B, Cotton A, Lam J, et al. Proteasome inhibition targets the KMT2A transcriptional complex in acute lymphoblastic leukemia. Nat Commun. 2023;14:809 pubmed publisher
Kumar P, Khadirnaikar S, Bhandari N, Chatterjee A, Shukla S. An epithelial-mesenchymal plasticity signature identifies two novel LncRNAs with the opposite regulation. Front Cell Dev Biol. 2022;10:885785 pubmed publisher
Parekh P, Mu Q, Badachhape A, Bhavane R, Srivastava M, Devkota L, et al. A surrogate marker for very early-stage tau pathology is detectable by molecular magnetic resonance imaging. Theranostics. 2022;12:5504-5521 pubmed publisher
Auwercx J, Kischel P, Lefebvre T, Jonckheere N, Vanlaeys A, Gu xe9 nin S, et al. TRPM7 Modulates Human Pancreatic Stellate Cell Activation. Cells. 2022;11: pubmed publisher
Wang H, Luo Y, Chu Z, Ni T, Ou S, Dai X, et al. Poria Acid, Triterpenoids Extracted from Poria cocos, Inhibits the Invasion and Metastasis of Gastric Cancer Cells. Molecules. 2022;27: pubmed publisher
Ye G, Huang M, Li Y, Ouyang J, Chen M, Wen Q, et al. The FAP α -activated prodrug Z-GP-DAVLBH inhibits the growth and pulmonary metastasis of osteosarcoma cells by suppressing the AXL pathway. Acta Pharm Sin B. 2022;12:1288-1304 pubmed publisher
Chu Z, Luo Y, Ni T, Zhu M, Feng X, Liu Y, et al. Betulonic Acid, as One of the Active Components of the Celastrus orbiculatus Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In Vitro. Molecules. 2022;27: pubmed publisher
Izdebska M, Zieli x144 ska W, Krajewski A, Ha x142 as Wi x15b niewska M, Miko x142 ajczyk K, Gagat M, et al. Downregulation of MMP-9 Enhances the Anti-Migratory Effect of Cyclophosphamide in MDA-MB-231 and MCF-7 Breast Cancer Cell Lines. Int J Mol Sci. 2021;22: pubmed publisher
Daly A, Davidson M, Burdick J. 3D bioprinting of high cell-density heterogeneous tissue models through spheroid fusion within self-healing hydrogels. Nat Commun. 2021;12:753 pubmed publisher
Li Y, Gong H, Feng L, Mao D, Xiao Y, Wang Y, et al. Chondroitin polymerizing factor promotes breast carcinoma cell proliferation, invasion and migration and affects expression of epithelial-mesenchymal transition-related markers. FEBS Open Bio. 2021;11:423-434 pubmed publisher
Filippo Buono M, von Boehmer L, Strang J, Hoerstrup S, Emmert M, Nugraha B. Human Cardiac Organoids for Modeling Genetic Cardiomyopathy. Cells. 2020;9: pubmed publisher
Geng Y, Xu C, Wang Y, Zhang L. Quiescin Sulfhydryl Oxidase 1 Regulates the Proliferation, Migration and Invasion of Human Glioblastoma Cells via PI3K/Akt Pathway. Onco Targets Ther. 2020;13:5721-5729 pubmed publisher
Gritsenko P, Atlasy N, Dieteren C, Navis A, Venhuizen J, Veelken C, et al. p120-catenin-dependent collective brain infiltration by glioma cell networks. Nat Cell Biol. 2020;22:97-107 pubmed publisher
Resutek L, Hsieh A. The vacuolated morphology of chordoma cells is dependent on cytokeratin intermediate filaments. J Cell Physiol. 2019;234:3458-3468 pubmed publisher
von Brandenstein M, Schlosser M, Herden J, Heidenreich A, Störkel S, Fries J. MicroRNAs as Urinary Biomarker for Oncocytoma. Dis Markers. 2018;2018:6979073 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Vimentin Monoclonal Antibody (SP20)
Catalog # :
MA5-16409
Quantity :
500 uL
Price :
US 562.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Rabbit
Reactivity :
Human
Applications :
Flow Cytometry: 1:100, Immunocytochemistry: 1:1,000, Immunohistochemistry (Paraffin): 1:200, Western Blot: 1:1,000
Species :
Human
Clone :
SP20
Isotype :
IgG
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
Immunogen :
Recombinant human vimentin protein.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 1:100, Immunocytochemistry: 1:1,000, Immunohistochemistry (Paraffin): 1:200, Western Blot: 1:1,000
Aliases :
cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; HEL113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA