Mesothelin Monoclonal Antibody (SP74) | Invitrogen MA5-16378 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Mesothelin Monoclonal Antibody (SP74)

catalog :

MA5-16378

quantity :

500 uL

price :

US 612.00

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SP74

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry knockout validation, immunocytochemistry knockout validation

more info or order :

Invitrogen product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; human; 1:50; loading ...; fig 6a, 6b immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1b, 5b, 5c, 7a, 7b	Nunes M, Pacheco F, Coelho R, Leit xe3 o D, Ricardo S, David L. Mesothelin Expression Is Not Associated with the Presence of Cancer Stem Cell Markers SOX2 and ALDH1 in Ovarian Cancer. Int J Mol Sci. 2022;23: pubmed publisher
immunocytochemistry knockout validation; human; 1:50; loading ...; fig 2e immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1e	Coelho R, Ricardo S, Amaral A, Huang Y, Nunes M, Neves J, et al. Regulation of invasion and peritoneal dissemination of ovarian cancer by mesothelin manipulation. Oncogenesis. 2020;9:61 pubmed publisher
	Raykova D, Kermpatsou D, Malmqvist T, Harrison P, Sander M, Stiller C, et al. A method for Boolean analysis of protein interactions at a molecular level. Nat Commun. 2022;13:4755 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Mesothelin Monoclonal Antibody (SP74)

Catalog # :

MA5-16378

Quantity :

500 uL

Price :

US 612.00

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Rabbit

Reactivity :

Human

Applications :

Immunohistochemistry (Paraffin): 1:100, Western Blot: 0.32 ug/mL

Species :

Human

Clone :

SP74

Isotype :

IgG

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Originally identified as a differentiation antigen of mesotheliomas, ovarian cystadenocarcinomas, and pancreatic adenocarcinomas, Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored, cell-surface glycoprotein predominantly secreted by cells of the mesothelium. Although Mesothelin is expressed at restricted levels by normal mesothelial cells of the pleural,pericardial, and peritoneal membranes, aberrant expression has been documented in the aforementioned cancers, as well as in endometriod uterine adenocarcinomas and squamous cell carcinomas of the esophagus, stomach, lung, and cervix. Proteolytic cleavage of Mesothelin yields a soluble, polypeptide fragment designated megakaryocyte potentiating factor (MPF) based on its ability to stimulate megakaryocyte colony-forming activity of murine interleukin-3 in murine bone marrow cell cultures. Originally isolated from the HPC-Y5 pancreatic cell line, MPF has been suggested to play a role in the proliferation and differentiation of megakaryoctyes, and the regulation of resultant platelet production. While the biological functions of both Mesothelin and MPF remain speculative, high-levels of expression in cancerous tissues compared to limited distribution in normal tissues strongly suggests their involvement in tumorigenesis. Both have been demonstrated to promote tumor cell proliferation, migration,anchorage-independent growth, and tumor progression; demonstrating their involvement in heterotypic cell adhesion and the metastatic spread of cancer.

Immunogen :

Synthetic peptide corresponding to C-terminus of human mesothelin protein.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:100, Western Blot: 0.32 ug/mL

Aliases :

CAK1; CAK1 antigen; megakaryocyte potentiating factor; Megakaryocyte-potentiating factor; Mes; Mesothelin; Mesothelin, cleaved form; MPF; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; SMRP; soluble MPF mesothelin related protein

more info or order :

Invitrogen product webpage