Carbonic Anhydrase IX Monoclonal Antibody (GT12) | Invitrogen MA5-16318 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Carbonic Anhydrase IX Monoclonal Antibody (GT12)

catalog :

MA5-16318

quantity :

100 µL

price :

US 449.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GT12

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; fig 8 western blot; human; 1:250; fig 6	MÃ¼ller W, SchrÃ¶der H, Tolba E, Diehl Seifert B, Wang X. Mineralization of bone-related SaOS-2 cells under physiological hypoxic conditions. FEBS J. 2016;283:74-87 pubmed publisher
	Cazzamalli S, Corso A, Neri D. Linker stability influences the anti-tumor activity of acetazolamide-drug conjugates for the therapy of renal cell carcinoma. J Control Release. 2017;246:39-45 pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody (MA5-16318) at a dilution of 1:2000. The hypoxic regions of the tumor show positive CAIX staining.

image 2 :

Western Blot analysis using anti-CAIX Monoclonal Antibody on the following cells: (30 µg HeLa whole cell lysate) A: 24 hr Untreated B: 24 hr treatment with 100µM CoCl2 C: 24 hr treatment with 200µM CoCl2 D: 48 hr Untreated E: 48 hr treatment with 100µM CoCl2 F: 48 hr treatment with 200µM CoCl2. Primary antibody (MA5-16318) diluted at 1:2000

image 3 :

Flow cytometry on HeLa cells stained with anti-CAIX antibody (MA5-16318). HeLa cells were untreated (green) or treated with 200?M CoCl2 (pink) for 48 hr.

product information

Product Type :

Antibody

Product Name :

Carbonic Anhydrase IX Monoclonal Antibody (GT12)

Catalog # :

MA5-16318

Quantity :

100 µL

Price :

US 449.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 1:100-1:1,000, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunohistochemistry: Assay-dependent, Immunoprecipitation: 2-10 µg/mg of lysate, Western Blot: 1:100-1:1,000

Species :

Human

Clone :

GT12

Isotype :

IgG2b

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

Immunogen :

linear repetitive epitope in the PG region and allows for selective detection of both native and denatured Human CA IX.

Format :

Liquid

Applications w/Dilutions :

Aliases :

Ca9; CA-9; CAIX; CA-IX; Car9; Carbonate dehydratase IX; carbonic anhydrase 9; Carbonic anhydrase IX; carbonic dehydratase; G250; Membrane antigen MN; membrane antigen MN homolog; MN; MN/CA9; P54 / 58N; P54/58N; pMW1; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250

more info or order :

Invitrogen product webpage