TLR4 Monoclonal Antibody (HTA125) | Invitrogen MA5-16205 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TLR4 Monoclonal Antibody (HTA125)

catalog :

MA5-16205

quantity :

100 ug

price :

US 474.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HTA125

reactivity :

human, mouse, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2	Gren S, Janciauskiene S, Sandeep S, Jonigk D, Kvist P, Gerwien J, et al. The protease inhibitor cystatin C down-regulates the release of IL-? and TNF-? in lipopolysaccharide activated monocytes. J Leukoc Biol. 2016;100:811-822 pubmed
flow cytometry; human; loading ...; fig 4c	Steiner M, Hawranek T, Schneider M, Ferreira F, Horejs Hoeck J, Harrer A, et al. Elevated Toll-Like Receptor-Induced CXCL8 Secretion in Human Blood Basophils from Allergic Donors Is Independent of Toll-Like Receptor Expression Levels. PLoS ONE. 2016;11:e0149275 pubmed publisher
flow cytometry; human	Bayram M, Soylu A, Ates H, Kizildag S, Kavukcu S. TLR-4 polymorphisms and leukocyte TLR-4 expression in febrile UTI and renal scarring. Pediatr Nephrol. 2013;28:1827-35 pubmed publisher
blocking or activating experiments; human; fig 3	Ahmad U, Ali R, Lebastchi A, Qin L, Lo S, Yakimov A, et al. IFN-gamma primes intact human coronary arteries and cultured coronary smooth muscle cells to double-stranded RNA- and self-RNA-induced inflammatory responses by upregulating TLR3 and melanoma differentiation-associated gene 5. J Immunol. 2010;185:1283-94 pubmed publisher
flow cytometry; human; fig 1	Tunheim G, Schjetne K, Fredriksen A, Sandlie I, Bogen B. Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes. J Leukoc Biol. 2005;77:303-10 pubmed
	Martínez García M, Ojeda Ojeda M, Rodríguez Martín E, Insenser M, Moncayo S, Alvarez Blasco F, et al. TLR2 and TLR4 Surface and Gene Expression in White Blood Cells after Fasting and Oral Glucose, Lipid and Protein Challenges: Influence of Obesity and Sex Hormones. Biomolecules. 2020;10: pubmed publisher
	Curtale G, Renzi T, Mirolo M, Drufuca L, Albanese M, De Luca M, et al. Multi-Step Regulation of the TLR4 Pathway by the miR-125a~99b~let-7e Cluster. Front Immunol. 2018;9:2037 pubmed publisher
	Domon H, Nagai K, Maekawa T, Oda M, Yonezawa D, Takeda W, et al. Neutrophil Elastase Subverts the Immune Response by Cleaving Toll-Like Receptors and Cytokines in Pneumococcal Pneumonia. Front Immunol. 2018;9:732 pubmed publisher
	Bartlett D, Willis L, Slentz C, Hoselton A, KELLY L, Huebner J, et al. Ten weeks of high-intensity interval walk training is associated with reduced disease activity and improved innate immune function in older adults with rheumatoid arthritis: a pilot study. Arthritis Res Ther. 2018;20:127 pubmed publisher
	Wisgrill L, Wessely I, Spittler A, Forster Waldl E, Berger A, Sadeghi K. Human lactoferrin attenuates the proinflammatory response of neonatal monocyte-derived macrophages. Clin Exp Immunol. 2018;192:315-324 pubmed publisher
	Hoffman D, Amorim J, DeClue A. Immune Function in Critically Ill Dogs. J Vet Intern Med. 2017;: pubmed publisher
	Bzowska M, Nogieć A, Bania K, Zygmunt M, Zarebski M, Dobrucki J, et al. Involvement of cell surface 90 kDa heat shock protein (HSP90) in pattern recognition by human monocyte-derived macrophages. J Leukoc Biol. 2017;102:763-774 pubmed publisher
	Di Candia L, Gomez E, Venereau E, Chachi L, Kaur D, Bianchi M, et al. HMGB1 is upregulated in the airways in asthma and potentiates airway smooth muscle contraction via TLR4. J Allergy Clin Immunol. 2017;140:584-587.e8 pubmed publisher
	Bazzi S, Modjtahedi H, Mudan S, Akle C, Bahr G. Analysis of the immunomodulatory properties of two heat-killed mycobacterial preparations in a human whole blood model. Immunobiology. 2015;220:1293-304 pubmed publisher
	Nazli A, Kafka J, Ferreira V, Anipindi V, Mueller K, Osborne B, et al. HIV-1 gp120 induces TLR2- and TLR4-mediated innate immune activation in human female genital epithelium. J Immunol. 2013;191:4246-58 pubmed publisher
	Li W, Katz B, Spinola S. Haemophilus ducreyi lipooligosaccharides induce expression of the immunosuppressive enzyme indoleamine 2,3-dioxygenase via type I interferons and tumor necrosis factor alpha in human dendritic cells. Infect Immun. 2011;79:3338-47 pubmed publisher

product information

Product Type :

Antibody

Product Name :

TLR4 Monoclonal Antibody (HTA125)

Catalog # :

MA5-16205

Quantity :

100 ug

Price :

US 474.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Canine, Human, Mouse

Applications :

Flow Cytometry: 0.25 ug x 10^6 cells, Functional Assay: Assay-Dependent, Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Paraffin): Assay-Dependent, Immunoprecipitation: 1:10-1:500, In vitro Assay: Assay-Dependent, Neutralization: Assay-Dependent, Western Blot: 1-2 ug/mL

Species :

Canine, Human, Mouse

Clone :

HTA125

Isotype :

IgG2a

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

Immunogen :

Ba/F3 cell line expressing human TLR4 cell surface antigen

Format :

Liquid

Applications w/Dilutions :

Aliases :

ARMD10; CD284; EGK_07381; homolog of Drosophila toll; hToll; lipopolysaccharide response; Lps; Ly87; Ran/M1; Rasl2-8; Tlr4; TLR-4; TOLL; toll like receptor 4; toll/interleukin-l receptor (TIR) domain; toll4; toll-like receptor 4; toll-like receptor 4 immunity-related protein; Toll-like receptor 4-like protein; Toll-like receptor4 protein

more info or order :

Invitrogen product webpage