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antibody

IkB alpha Monoclonal Antibody (6A920)

MA5-16152

100 ug

US 524.00

monoclonal

mouse

nonconjugated

6A920

The same clone is also sold as:

• Novus Biologicals: NBP2-80787, NB100-56507
• OriGene: TA336372
• Rockland Immunochemicals: 200-301-H80

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

IkB alpha Monoclonal Antibody (6A920)

MA5-16152

100 ug

US 524.00

Monoclonal

Protein G

Mouse

Human, Mouse, Rat

Flow Cytometry: 0.25-1 ug per million cells, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: 1 ug/mL, Western Blot: 1-2 ug/mL

Human, Mouse, Rat

6A920

IgG1, kappa

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.

A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.

Liquid

Flow Cytometry: 0.25-1 ug per million cells, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: 1 ug/mL, Western Blot: 1-2 ug/mL

AI462015; ECI-6; ECI-6/IKBA; I kappa B-alpha; I(Kappa)B(alpha); I79_018146; IkappaB alpha; IkappaBalpha; I-kappaBalpha; I-kappa-B-alpha; Ikba; IKBalpha; IKB-alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; MAD3; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappaB inhibitor alpha; NF-kappa-B inhibitor alpha; NFKB inhibitor alpha; NFKBI; Nfkbia; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; Rel-associated pp40; REL-associated protein pp40; RL/IF-1