PHD3 Monoclonal Antibody (EG188e/d5) | Invitrogen MA5-16144 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PHD3 Monoclonal Antibody (EG188e/d5)

catalog :

MA5-16144

quantity :

200 µL

price :

US 425

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

EG188e/d5

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; loading ...; fig 2E;2D western blot; mouse; 1:500; loading ...; fig 3	Mao K, You C, Lei D, Zhang H. Potential regulation of glioma through the induction of apoptosis signaling via Egl-9 family hypoxia-inducible factor 3. Oncol Lett. 2017;13:893-897 pubmed publisher

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product information

Product Type :

Antibody

Product Name :

PHD3 Monoclonal Antibody (EG188e/d5)

Catalog # :

MA5-16144

Quantity :

200 µL

Price :

US 425

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Rat

Applications :

Immunohistochemistry (Paraffin): 1:400, Western Blot: 10 µg/mL

Species :

Human, Rat

Clone :

EG188e/d5

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Target proteins are preferentially recognized via a LXXLAP motif.

Immunogen :

Partial recombinant human PHD3 protein (UniProt accession number Q9H6Z9)

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:400, Western Blot: 10 µg/mL

Aliases :

2610021G09Rik; AI505553; AI648162; Egl nine homolog 3; egl nine homolog 3, mitochondrial; egl nine-like protein 3 isoform; egl-9 family hypoxia inducible factor 3; egl-9 family hypoxia-inducible factor 3; EGLN3; factor-responsive smooth muscle protein; FLJ21620; HIF prolyl hydroxylase 3; HIFP4H3; Hif-p4h-3; HIFPH3; HIF-PH3; HIF-prolyl hydroxylase 3; HPH-1; HPH-3; hypoxia-inducible factor prolyl hydroxylase 3; MGC125998; MGC125999; P4H3; Phd3; PHD-3; prolyl hydroxylase domain-containing protein 3; Sm20; SM-20

more info or order :

Invitrogen product webpage