Desmoglein 3 Monoclonal Antibody (5G11) | Invitrogen MA5-16025 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Desmoglein 3 Monoclonal Antibody (5G11)

catalog :

MA5-16025

quantity :

100 uL

price :

US 484.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5G11

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, dot blot

more info or order :

Invitrogen product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2c	Ebert L, Tan L, Johan M, Min K, Cockshell M, Parham K, et al. A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis. Angiogenesis. 2016;19:463-86 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Hartlieb E, Rötzer V, Radeva M, Spindler V, Waschke J. Desmoglein 2 compensates for desmoglein 3 but does not control cell adhesion via regulation of p38 mitogen-activated protein kinase in keratinocytes. J Biol Chem. 2014;289:17043-53 pubmed publisher
immunocytochemistry; human	Rötzer V, Breit A, Waschke J, Spindler V. Adducin is required for desmosomal cohesion in keratinocytes. J Biol Chem. 2014;289:14925-40 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50; fig 1	Fang W, Chen B, Xu X, Liao L, Wu Z, Wu J, et al. Altered expression and localization of desmoglein 3 in esophageal squamous cell carcinoma. Acta Histochem. 2014;116:803-9 pubmed publisher
western blot; human	Stahley S, Saito M, Faundez V, Koval M, Mattheyses A, Kowalczyk A. Desmosome assembly and disassembly are membrane raft-dependent. PLoS ONE. 2014;9:e87809 pubmed publisher
immunohistochemistry; human	Pontiggia L, Biedermann T, Böttcher Haberzeth S, Oliveira C, Braziulis E, Klar A, et al. De novo epidermal regeneration using human eccrine sweat gland cells: higher competence of secretory over absorptive cells. J Invest Dermatol. 2014;134:1735-1742 pubmed publisher
immunocytochemistry; human; fig 3 western blot; human; fig 3	Mao X, Li H, Sano Y, Gaestel M, Mo Park J, Payne A. MAPKAP kinase 2 (MK2)-dependent and -independent models of blister formation in pemphigus vulgaris. J Invest Dermatol. 2014;134:68-76 pubmed publisher
immunocytochemistry; human; fig 3 immunohistochemistry; mouse; fig 2	Mao X, Sano Y, Park J, Payne A. p38 MAPK activation is downstream of the loss of intercellular adhesion in pemphigus vulgaris. J Biol Chem. 2011;286:1283-91 pubmed publisher
immunocytochemistry; human; fig 3 immunohistochemistry; human; fig 1 western blot; human; fig 2	Mao X, Nagler A, Farber S, Choi E, Jackson L, Leiferman K, et al. Autoimmunity to desmocollin 3 in pemphigus vulgaris. Am J Pathol. 2010;177:2724-30 pubmed publisher
immunocytochemistry; human; 1:100	Schlegel N, Meir M, Heupel W, Holthöfer B, Leube R, Waschke J. Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity. Am J Physiol Gastrointest Liver Physiol. 2010;298:G774-83 pubmed publisher
western blot; human; fig 5	Mao X, Choi E, Payne A. Disruption of desmosome assembly by monovalent human pemphigus vulgaris monoclonal antibodies. J Invest Dermatol. 2009;129:908-18 pubmed publisher
immunoprecipitation; human; fig 2	Kanno M, Aoyama Y, Isa Y, Yamamoto Y, Kitajima Y. P120 catenin is associated with desmogleins when desmosomes are assembled in high-Ca2+ medium but not when disassembled in low-Ca2+ medium in DJM-1 cells. J Dermatol. 2008;35:317-24 pubmed publisher
immunohistochemistry - frozen section; human	Schlötzer Schrehardt U, Dietrich T, Saito K, Sorokin L, Sasaki T, Paulsson M, et al. Characterization of extracellular matrix components in the limbal epithelial stem cell compartment. Exp Eye Res. 2007;85:845-60 pubmed
immunohistochemistry - paraffin section; human; 1:100; tbl 1	Yang L, Shirakata Y, Shudou M, Dai X, Tokumaru S, Hirakawa S, et al. New skin-equivalent model from de-epithelialized amnion membrane. Cell Tissue Res. 2006;326:69-77 pubmed
immunohistochemistry - paraffin section; human; tbl 1	Kurzen H, Münzing I, Hartschuh W. Expression of desmosomal proteins in squamous cell carcinomas of the skin. J Cutan Pathol. 2003;30:621-30 pubmed
	Koch P, Webb S, Gugger J, Salois M, Koster M. Differentiation of Human Induced Pluripotent Stem Cells into Keratinocytes. Curr Protoc. 2022;2:e408 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Desmoglein 3 Monoclonal Antibody (5G11)

Catalog # :

MA5-16025

Quantity :

100 uL

Price :

US 484.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

Dot Blot: Assay-Dependent, Flow Cytometry: 1 ug per million cells, Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: 1:10-1:500, Western Blot: 1:500-1:1,000

Species :

Human

Clone :

5G11

Isotype :

IgG1

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominately expressed in stratified squamous epithelium, including the skin, oral mucosa, and esophagus. Desmoglein 3 has been characterized as the auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

Immunogen :

Human Desmoglein 3 recombinant protein.

Format :

Liquid

Applications w/Dilutions :

Dot Blot: Assay-Dependent, Flow Cytometry: 1 ug per million cells, Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: 1:10-1:500, Western Blot: 1:500-1:1,000

Aliases :

130 kDa pemphigus vulgaris antigen; 130 kDa pemphigus vulgaris antigen homolog; bal; balding; Cadherin family member 6; CDHF6; desmoglein 3; desmoglein-3; DKFZp686P23184; Dsg3; PVA

more info or order :

Invitrogen product webpage