HIF1A Monoclonal Antibody (H1alpha67), DyLight™ 488 | Invitrogen MA5-16009 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HIF1A Monoclonal Antibody (H1alpha67), DyLight™ 488

catalog :

MA5-16009

quantity :

100 µL

price :

US 449.00

clonality :

monoclonal

host :

mouse

conjugate :

DyLight488

clone name :

H1alpha67

reactivity :

human, mouse, rat, dogs, bovine, pigs , cat, domestic sheep, Xenopus laevis, domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 35

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human	Stofas A, Levidou G, Piperi C, Adamopoulos C, Dalagiorgou G, Bamias A, et al. The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma. BMC Cancer. 2014;14:149 pubmed publisher
immunohistochemistry; human; 1:10,000	Choueiri T, Fay A, Gagnon R, Lin Y, Bahamón B, Brown V, et al. The role of aberrant VHL/HIF pathway elements in predicting clinical outcome to pazopanib therapy in patients with metastatic clear-cell renal cell carcinoma. Clin Cancer Res. 2013;19:5218-26 pubmed publisher
immunohistochemistry; human; 1:100	van den Berg L, Crane L, van Oosten M, van Dam G, Simons A, Hofker H, et al. Analysis of biomarker expression in severe endometriosis and determination of possibilities for targeted intraoperative imaging. Int J Gynaecol Obstet. 2013;121:35-40 pubmed publisher
immunohistochemistry; human; 1:50	Park J, Jin Y, Lee Y, Lee J, Lee Y, Ko Y, et al. KAI1 suppresses HIF-1? and VEGF expression by blocking CDCP1-enhanced Src activation in prostate cancer. BMC Cancer. 2012;12:81 pubmed publisher
immunohistochemistry; rat; 1:200	Hsieh Y, Chou L, Chang P, Yang C, Kao M, Hong C. Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1? (HIF-1?). J Comp Neurol. 2012;520:2903-16 pubmed publisher
immunohistochemistry; human	Facciabene A, Peng X, Hagemann I, Balint K, Barchetti A, Wang L, et al. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature. 2011;475:226-30 pubmed publisher
immunohistochemistry; mouse	Ahn J, Joo H, Suh J, Kim H, So H, Chung J. Effects of cigarette smoking on hearing recovery from noise-induced temporary hearing threshold shifts in mice. Otol Neurotol. 2011;32:926-32 pubmed publisher
immunohistochemistry; human	Takagi Y, Kikuta K, Moriwaki T, Aoki T, Nozaki K, Hashimoto N, et al. Expression of thioredoxin-1 and hypoxia inducible factor-1? in cerebral arteriovenous malformations: Possible role of redox regulatory factor in neoangiogenic property. Surg Neurol Int. 2011;2:61 pubmed publisher
immunohistochemistry; human; 1:20	Kafousi M, Vrekoussis T, Tsentelierou E, Pavlakis K, Navrozoglou I, Dousias V, et al. Immunohistochemical study of the angiogenetic network of VEGF, HIF1?, VEGFR-2 and endothelial nitric oxide synthase (eNOS) in human breast cancer. Pathol Oncol Res. 2012;18:33-41 pubmed publisher
immunohistochemistry; human; 1:20	Zhang L, Liu Q, Lu L, Zhao X, Gao X, Wang Y. Astragaloside IV stimulates angiogenesis and increases hypoxia-inducible factor-1? accumulation via phosphatidylinositol 3-kinase/Akt pathway. J Pharmacol Exp Ther. 2011;338:485-91 pubmed publisher
immunohistochemistry; mouse	Qing G, Skuli N, Mayes P, Pawel B, Martinez D, Maris J, et al. Combinatorial regulation of neuroblastoma tumor progression by N-Myc and hypoxia inducible factor HIF-1alpha. Cancer Res. 2010;70:10351-61 pubmed publisher
western blot; human	Sumiyoshi M, Kimura Y. Enhancing effects of a chromone glycoside, eucryphin, isolated from Astilbe rhizomes on burn wound repair and its mechanism. Phytomedicine. 2010;17:820-9 pubmed publisher
western blot; rat	Ali M, Janic B, Babajani Feremi A, Varma N, Iskander A, Anagli J, et al. Changes in vascular permeability and expression of different angiogenic factors following anti-angiogenic treatment in rat glioma. PLoS ONE. 2010;5:e8727 pubmed publisher
western blot; mouse	Matsumoto K, Obara N, Ema M, Horie M, Naka A, Takahashi S, et al. Antitumor effects of 2-oxoglutarate through inhibition of angiogenesis in a murine tumor model. Cancer Sci. 2009;100:1639-47 pubmed publisher
immunohistochemistry; human	Deniz H, Karakok M, Yagci F, Guldur M. Evaluation of relationship between HIF-1alpha immunoreactivity and stage, grade, angiogenic profile and proliferative index in bladder urothelial carcinomas. Int Urol Nephrol. 2010;42:103-7 pubmed publisher
immunohistochemistry; mouse; 1:50	Bailey S, Mantena S, Millender Swain T, Cakir Y, Jhala N, Chhieng D, et al. Ethanol and tobacco smoke increase hepatic steatosis and hypoxia in the hypercholesterolemic apoE(-/-) mouse: implications for a "multihit" hypothesis of fatty liver disease. Free Radic Biol Med. 2009;46:928-38 pubmed
immunohistochemistry; human	Arbab A, Janic B, Knight R, Anderson S, Pawelczyk E, Rad A, et al. Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings. FASEB J. 2008;22:3234-46 pubmed publisher
immunohistochemistry; human; 1:50	Vakkala M, Laurila J, Saarnio J, Koivukangas V, Syrjala H, Karttunen T, et al. Cellular turnover and expression of hypoxic-inducible factor in acute acalculous and calculous cholecystitis. Crit Care. 2007;11:R116 pubmed
immunocytochemistry; rat; 1:200	Agrawal A, Guttapalli A, Narayan S, Albert T, Shapiro I, Risbud M. Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk. Am J Physiol Cell Physiol. 2007;293:C621-31 pubmed
immunohistochemistry; human; 1:100	Sihto H, Tynninen O, Butzow R, Saarialho Kere U, Joensuu H. Endothelial cell KIT expression in human tumours. J Pathol. 2007;211:481-8 pubmed
western blot; human; 1:1000	Pore N, Jiang Z, Shu H, Bernhard E, Kao G, Maity A. Akt1 activation can augment hypoxia-inducible factor-1alpha expression by increasing protein translation through a mammalian target of rapamycin-independent pathway. Mol Cancer Res. 2006;4:471-9 pubmed
immunohistochemistry; domestic rabbit; 1:4000	Mori S, Akagi M, Kikuyama A, Yasuda Y, Hamanishi C. Axial shortening during distraction osteogenesis leads to enhanced bone formation in a rabbit model through the HIF-1alpha/vascular endothelial growth factor system. J Orthop Res. 2006;24:653-63 pubmed
immunocytochemistry; human; 1:200	Risbud M, Guttapalli A, Stokes D, Hawkins D, Danielson K, Schaer T, et al. Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment. J Cell Biochem. 2006;98:152-9 pubmed
immunohistochemistry; human	Huang G, Yang L, Lu W. Expression of hypoxia-inducible factor 1alpha and vascular endothelial growth factor in hepatocellular carcinoma: Impact on neovascularization and survival. World J Gastroenterol. 2005;11:1705-8 pubmed
	Wang W, Cheng Y, Yu P, Wang H, Zhang Y, Xu H, et al. Perfluorocarbon regulates the intratumoural environment to enhance hypoxia-based agent efficacy. Nat Commun. 2019;10:1580 pubmed publisher
	Wang Y, Pakunlu R, Tsao W, Pozharov V, Minko T. Bimodal effect of hypoxia in cancer: role of hypoxia inducible factor in apoptosis. Mol Pharm. 2004;1:156-65 pubmed
	Chung J, Kang H, Shin J, Kim J. Accumulation of hypoxia-inducible factor-1alpha in mouse inner ear by noise stimulation. Neuroreport. 2004;15:2353-6 pubmed
	Acs G, Xu X, Chu C, Acs P, Verma A. Prognostic significance of erythropoietin expression in human endometrial carcinoma. Cancer. 2004;100:2376-86 pubmed
	Tuomisto T, Rissanen T, Vajanto I, Korkeela A, Rutanen J, Yla Herttuala S. HIF-VEGF-VEGFR-2, TNF-alpha and IGF pathways are upregulated in critical human skeletal muscle ischemia as studied with DNA array. Atherosclerosis. 2004;174:111-20 pubmed
	Ma W, Tessarollo L, Hong S, Baba M, Southon E, Back T, et al. Hepatic vascular tumors, angiectasis in multiple organs, and impaired spermatogenesis in mice with conditional inactivation of the VHL gene. Cancer Res. 2003;63:5320-8 pubmed
	Kurokawa T, Miyamoto M, Kato K, Cho Y, Kawarada Y, Hida Y, et al. Overexpression of hypoxia-inducible-factor 1alpha(HIF-1alpha) in oesophageal squamous cell carcinoma correlates with lymph node metastasis and pathologic stage. Br J Cancer. 2003;89:1042-7 pubmed
	Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M. Enhanced overexpression of an HIF-1/hypoxia-related protein in cancer cells. Environ Health Perspect. 2002;110 Suppl 5:783-8 pubmed
	Cangul H, Salnikow K, Yee H, Zagzag D, Commes T, Costa M. Enhanced expression of a novel protein in human cancer cells: a potential aid to cancer diagnosis. Cell Biol Toxicol. 2002;18:87-96 pubmed
	Kim M, Kwon H, Lee Y, Baek J, Jang J, Lee S, et al. Histone deacetylases induce angiogenesis by negative regulation of tumor suppressor genes. Nat Med. 2001;7:437-43 pubmed
	Yamashita K, Discher D, Hu J, Bishopric N, Webster K. Molecular regulation of the endothelin-1 gene by hypoxia. Contributions of hypoxia-inducible factor-1, activator protein-1, GATA-2, AND p300/CBP. J Biol Chem. 2001;276:12645-53 pubmed

product information

Product Type :

Antibody

Product Name :

HIF1A Monoclonal Antibody (H1alpha67), DyLight™ 488

Catalog # :

MA5-16009

Quantity :

100 µL

Price :

US 449.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Canine, Feline, Ferret, Human, Mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat, Xenopus laevis

Applications :

ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Frozen): 1:100, Immunohistochemistry (Paraffin): Assay-Dependent, Western Blot: 1:500

Species :

Bovine, Canine, Feline, Ferret, Human, Mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat, Xenopus laevis

Clone :

H1alpha67

Isotype :

IgG2b

Storage :

4° C, store in dark

Description :

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

Immunogen :

Fusion protein containing amino acids 432-528 of human HIF-1alpha.

Format :

Liquid

Applications w/Dilutions :

Aliases :

AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a

more info or order :

Invitrogen product webpage