c-Jun Monoclonal Antibody (4H9) | Invitrogen MA5-15889 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

c-Jun Monoclonal Antibody (4H9)

catalog :

MA5-15889

quantity :

100 µL

price :

US 474

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4H9

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; fig 6	Marescotti D, Gonzalez Suarez I, Acali S, Johne S, Laurent A, Frentzel S, et al. High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC). J Vis Exp. 2016;: pubmed publisher

image

image 1 :

Immunofluorescence analysis of Hela cells using MA5-15889 anti-c-Jun antibody (green). Blue staining is DRAQ5 fluorescent DNA dye and Red staining of Actin filaments was Alexa Fluor-555 phalloidin.

image 2 :

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using MA5-15889 anti-c-Jun antibody with DAB staining.

product information

Product Type :

Antibody

Product Name :

c-Jun Monoclonal Antibody (4H9)

Catalog # :

MA5-15889

Quantity :

100 µL

Price :

US 474

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ELISA: 1:10,000, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Human, Mouse

Clone :

4H9

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

Immunogen :

Purified recombinant fragment of human c-Jun expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

Activator protein 1; AH119; AP1; AP-1; Avian sarcoma virus 17 (v-jun) oncogene homolog; cjun; c-Jun; c-jun transcription factor; enhancer-binding protein AP1; I79_009953; immediate early; JUN; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; Jun proto-oncogene, AP-1 transcription factor subunit; Junc; JUND; p39; proto-oncogene c-Jun; Rjg-9; transcription factor; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog

more info or order :

Invitrogen product webpage