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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
MMP9 Monoclonal Antibody (5G3)
catalog :
MA5-15886
quantity :
100 µL
price :
US 510
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5G3
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 23
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:500; loading ...; fig 5a
Ouyang X, Feng L, Yao L, Xiao Y, Hu X, Zhang G, et al. Testicular orphan receptor 4 (TR4) promotes papillary thyroid cancer invasion via activating circ-FNLA/miR-149-5p/MMP9 signaling. Mol Ther Nucleic Acids. 2021;24:755-767 pubmed publisher
  • western blot; mouse; loading ...; fig 4b
Yi R, Zhang J, Sun P, Qian Y, Zhao X. Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice. Molecules. 2019;24: pubmed publisher
  • western blot; human; loading ...; fig s5
Li Y, Liu C, Su T, Cheng H, Jeng Y, Lin H, et al. Characterization of metastatic tumor antigen 1 and its interaction with hepatitis B virus X protein in NF-κB signaling and tumor progression in a woodchuck hepatocellular carcinoma model. Oncotarget. 2016;7:47173-47185 pubmed publisher
  • immunocytochemistry; human; 1:600; fig 7, 8
Naegelen I, Plançon S, Nicot N, Kaoma T, Muller A, Vallar L, et al. An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1α, IL-1β, IL-12b, and CCL4 from differentiated HL-60 cells. J Leukoc Biol. 2015;97:557-71 pubmed publisher
Zhao P, Sun T, Lyu C, Liang K, Niu Y, Zhang Y, et al. Scar-Degrading Endothelial Cells as a Treatment for Advanced Liver Fibrosis. Adv Sci (Weinh). 2023;10:e2203315 pubmed publisher
Juin S, Pushpakumar S, Tyagi S, Sen U. Glucosidase inhibitor, Nimbidiol ameliorates renal fibrosis and dysfunction in type-1 diabetes. Sci Rep. 2022;12:21707 pubmed publisher
Yang J, Liu Z, Liu B, Sun L. Silencing of circCYP51A1 represses cell progression and glycolysis by regulating miR-490-3p/KLF12 axis in osteosarcoma under hypoxia. J Bone Oncol. 2022;37:100455 pubmed publisher
Kim S, Nagar H, Lee I, Choi S, Piao S, Jeon B, et al. Antitumor Potential of Sericite Treatment Mediated by Cell Cycle Arrest in Triple-Negative MDA-MB231 Breast Cancer Cells. Evid Based Complement Alternat Med. 2022;2022:2885293 pubmed publisher
Chilunda V, Weiselberg J, Martínez Meza S, Mhamilawa L, Cheney L, Berman J. Methamphetamine induces transcriptional changes in cultured HIV-infected mature monocytes that may contribute to HIV neuropathogenesis. Front Immunol. 2022;13:952183 pubmed publisher
Karagülle O, Yurttas A. Ozone combined with doxorubicin exerts cytotoxic and anticancer effects on Luminal-A subtype human breast cancer cell line. Rev Assoc Med Bras (1992). 2022;68:507-513 pubmed publisher
Shen X, Li L, He Y, Lv X, Ma J. EIF5A2 Is Involved in the Biological Process of Cervical Cancer Cells through AGR2. Pharmacology. 2022;107:376-385 pubmed publisher
Wang L, Zhang Z, Wang L, Wei H, Zhang T, Zhang L, et al. TGF-β1/SH2B3 axis regulates anoikis resistance and EMT of lung cancer cells by modulating JAK2/STAT3 and SHP2/Grb2 signaling pathways. Cell Death Dis. 2022;13:472 pubmed publisher
Yan P, Sun C, Luan L, Han J, Qu Y, Zhou C, et al. Hsa_circ_0134111 promotes intervertebral disc degeneration via sponging miR-578. Cell Death Discov. 2022;8:55 pubmed publisher
Juin S, Pushpakumar S, Sen U. GYY4137 Regulates Extracellular Matrix Turnover in the Diabetic Kidney by Modulating Retinoid X Receptor Signaling. Biomolecules. 2021;11: pubmed publisher
Chu D, Li P, Li Y, Shi J, Huang S, Jiao P. Identification of circ_0058357 as a regulator in non-small cell lung cancer cells resistant to cisplatin by miR-361-3p/ABCC1 axis. Thorac Cancer. 2021;12:2894-2906 pubmed publisher
Nigro E, Mallardo M, Polito R, Scial xf2 F, Bianco A, Daniele A. Adiponectin and Leptin Exert Antagonizing Effects on HUVEC Tube Formation and Migration Modulating the Expression of CXCL1, VEGF, MMP-2 and MMP-9. Int J Mol Sci. 2021;22: pubmed publisher
Zhao X, Dong W, Luo G, Xie J, Liu J, Yu F. Silencing of hsa_circ_0009035 Suppresses Cervical Cancer Progression and Enhances Radiosensitivity through MicroRNA 889-3p-Dependent Regulation of HOXB7. Mol Cell Biol. 2021;41:e0063120 pubmed publisher
Pushpakumar S, Kundu S, Weber G, Sen U. Exogenous hydrogen sulfide and miR-21 antagonism attenuates macrophage-mediated inflammation in ischemia reperfusion injury of the aged kidney. GeroScience. 2021;: pubmed publisher
Chen T, Chen Z, Lian X, Wu W, Chu L, Zhang S, et al. MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/β-Catenin Signal Pathway. J Cancer. 2021;12:467-473 pubmed publisher
Cao Y, Lv W, Ding W, Li J. Sevoflurane inhibits the proliferation and invasion of hepatocellular carcinoma cells through regulating the PTEN/Akt/GSK‑3β/β‑catenin signaling pathway by downregulating miR‑25‑3p. Int J Mol Med. 2020;46:97-106 pubmed publisher
Chen F, Ni X, Chen L, Wang X, Xu J. miR-939-3p promotes epithelial-mesenchymal transition and may be used as a prognostic marker in hepatocellular carcinoma. Oncol Lett. 2020;19:2727-2732 pubmed publisher
Majumder S, Ren L, Pushpakumar S, Sen U. Hydrogen sulphide mitigates homocysteine-induced apoptosis and matrix remodelling in mesangial cells through Akt/FOXO1 signalling cascade. Cell Signal. 2019;61:66-77 pubmed publisher
Fouad H, Salem H, Ellakwa D, Abdel Hamid M. MMP-2 and MMP-9 as prognostic markers for the early detection of urinary bladder cancer. J Biochem Mol Toxicol. 2019;33:e22275 pubmed publisher
image
image 1 :
Invitrogen MA5-15886 image 1
Immunohistochemical analysis of paraffin-embedded brain tissues using MA5-15886 anti-MMP9 antibody with DAB staining.
product information
Product Type :
Antibody
Product Name :
MMP9 Monoclonal Antibody (5G3)
Catalog # :
MA5-15886
Quantity :
100 µL
Price :
US 510
Clonality :
Monoclonal
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
ELISA: 1:10,000, Flow Cytometry: 1:200-1:400, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000
Species :
Human, Mouse
Clone :
5G3
Isotype :
IgG2a
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.
Immunogen :
Purified recombinant fragment of human MMP9 expressed in E. Coli.
Format :
Liquid
Applications w/Dilutions :
ELISA: 1:10,000, Flow Cytometry: 1:200-1:400, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000
Aliases :
67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; 92kD gelatinase; 92kD type IV collagenase; 92kDa gelatinase; 92kDa type IV collagenase; 92-kDa type IV collagenase; AW743869; B/MMP9; Clg4b; collagenase type IVB; fj05a08; Gel B; gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; MANDP2; matrix metallo protease; matrix metallopeptidase 9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9; matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase); Matrix metalloproteinase9; matrix metalloproteinase-9; MMP; Mmp9; MMP-9; mmp9 protein; MMP-9 protein; MMPs; Preproform of 92-kDa type IV collagenase (MMP-9): N-terminal; pro-MMP-9; type IV collagenase; type V collagenase; wu:fb02g06; wu:fb07b05; wu:fi98c09; wu:fj05a08; ZFMMP-9; zgc:64165
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA