AIF Monoclonal Antibody (4E7E11) | Invitrogen MA5-15880 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

AIF Monoclonal Antibody (4E7E11)

catalog :

MA5-15880

quantity :

100 µg

price :

US 470.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4E7E11

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, proximity ligation assay

more info or order :

Invitrogen product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
proximity ligation assay; mouse; fig 3 immunoprecipitation; mouse; fig 1a immunocytochemistry; mouse; fig 2a western blot; mouse; fig 1c	Denuc A, NÃºÃ±ez E, Calvo E, Loureiro M, Miro Casas E, GuarÃ¡s A, et al. New protein-protein interactions of mitochondrial connexin 43 in mouse heart. J Cell Mol Med. 2016;20:794-803 pubmed publisher
western blot; human; 1:4000	Kettwig M, Schubach M, Zimmermann F, Klinge L, Mayr J, Biskup S, et al. From ventriculomegaly to severe muscular atrophy: expansion of the clinical spectrum related to mutations in AIFM1. Mitochondrion. 2015;21:12-8 pubmed publisher
	Ito G, Tatara Y, Itoh K, Yamada M, Yamashita T, Sakamoto K, et al. Novel dicarbonyl metabolic pathway via mitochondrial ES1 possessing glyoxalase III activity. BBA Adv. 2023;3:100092 pubmed publisher
	Rai R, Chandra V, Kennedy A, Zuna R, Benbrook D. Distinct mechanism of cervical cancer cell death caused by the investigational new drug SHetA2. Front Oncol. 2022;12:958536 pubmed publisher
	Karsai G, Steiner R, Kaech A, Lone M, von Eckardstein A, Hornemann T. Metabolism of HSAN1- and T2DM-associated 1-deoxy-sphingolipids inhibits the migration of fibroblasts. J Lipid Res. 2021;62:100122 pubmed publisher
	Chandra V, Rai R, Benbrook D. Utility and Mechanism of SHetA2 and Paclitaxel for Treatment of Endometrial Cancer. Cancers (Basel). 2021;13: pubmed publisher
	Susilo I, Maulida H, Alimsardjono L, Fauziah D, Pertiwi H. Apoptosis-Inducing Factor, Protein Expression, and Apoptosis Changes with Glutamine in Podocytes Cells Exposed with Cisplatin. Vet Med Int. 2021;2021:5599452 pubmed publisher
	Wang Z, Yuan C, Huang Y, Liu Z, Yu X, Lv C, et al. Decreased expression of apoptosis-inducing factor in renal cell carcinoma is associated with poor prognosis and reduced postoperative survival. Oncol Lett. 2019;18:2805-2812 pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using MA5-15880 anti-AIF antibody with DAB staining.

product information

Product Type :

Antibody

Product Name :

AIF Monoclonal Antibody (4E7E11)

Catalog # :

MA5-15880

Quantity :

100 µg

Price :

US 470.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

Immunocytochemistry: 1:50-1:100, Western Blot: 1:500

Species :

Human, Mouse, Non-human primate, Rat

Clone :

4E7E11

Isotype :

IgG2b

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.

Immunogen :

Purified recombinant fragment of human AIF expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:50-1:100, Western Blot: 1:500

Aliases :

AG1; AGR1; Aif; AIFM1; AIFsh2; apoptosis inducing factor mitochondria associated 1; apoptosis inducing factor, mitochondria associated 1; apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated 1; apoptosis-inducing factor, mitochondrion-associated, 1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; ER protein 18; ER protein 19; ERP18; ERP19; hAG-1; harlequin; Hq; hTLP19; MGC111425; mitochondrial apoptosis-inducing factor 1; NADMR; NAMSD; pcd8; PDCD8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376

more info or order :

Invitrogen product webpage