ASK1 Monoclonal Antibody (2E4) | Invitrogen MA5-15861 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

ASK1 Monoclonal Antibody (2E4)

catalog :

MA5-15861

quantity :

100 µL

price :

US 466.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2E4

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5c	Beauvais D, Jung O, Yang Y, Sanderson R, Rapraeger A. Syndecan-1 (CD138) Suppresses Apoptosis in Multiple Myeloma by Activating IGF1 Receptor: Prevention by SynstatinIGF1R Inhibits Tumor Growth. Cancer Res. 2016;76:4981-93 pubmed publisher
	Vo T, Malo Pueyo J, Wahni K, Ezerina D, Bolduc J, Messens J. Prdx1 Interacts with ASK1 upon Exposure to H2O2 and Independently of a Scaffolding Protein. Antioxidants (Basel). 2021;10: pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) using MA5-15861 anti-MAP3K5 antibody with DAB staining.

product information

Product Type :

Antibody

Product Name :

ASK1 Monoclonal Antibody (2E4)

Catalog # :

MA5-15861

Quantity :

100 µL

Price :

US 466.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: 1:10,000, Flow Cytometry: 1:200-1:400, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Human

Clone :

2E4

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

MAP3K5 (ASK1) is a serine/threonine kinase that is abundantly expressed in human heart and pancreas and are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activates c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death and a catalytically inactive form of ASK1 inhibited TNF-a-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

Immunogen :

Purified recombinant fragment of human MAP3K5 expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Flow Cytometry: 1:200-1:400, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

7420452D20Rik; apoptosis signal regulating kinase 1; apoptosis signal-regulating kinase 1; ASK; ASK 1; ASK1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP/ERK kinase kinase 5; Map3k5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; Mekk5; mitogen activated protein kinase kinase kinase 5; mitogen-activated protein kinase kinase kinase 5; RGD1306565; RP3-325F22.4

more info or order :

Invitrogen product webpage