OTX2 Monoclonal Antibody (1H12C4B5) | Invitrogen MA5-15854 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

OTX2 Monoclonal Antibody (1H12C4B5)

catalog :

MA5-15854

quantity :

100 µL

price :

US 510

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1H12C4B5

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; human; 1:200; fig 2c	Anastasaki C, Wilson A, Chen A, Wegscheid M, Gutmann D. Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization. STAR Protoc. 2022;3:101173 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig s4	Su J, Charalambakis N, Sabbagh U, Somaiya R, Monavarfeshani A, Guido W, et al. Retinal inputs signal astrocytes to recruit interneurons into visual thalamus. Proc Natl Acad Sci U S A. 2020;117:2671-2682 pubmed publisher
	Parichha A, Suresh V, Chatterjee M, Kshirsagar A, Ben Reuven L, Olender T, et al. Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate. Nat Commun. 2022;13:633 pubmed publisher
	Dottermusch M, Sumisławski P, Krevet J, Middelkamp M, Voß H, Siebels B, et al. Co-activation of Sonic hedgehog and Wnt signaling in murine retinal precursor cells drives ocular lesions with features of intraocular medulloepithelioma. Oncogenesis. 2021;10:78 pubmed publisher
	Wegscheid M, Anastasaki C, Hartigan K, Cobb O, Papke J, Traber J, et al. Patient-derived iPSC-cerebral organoid modeling of the 17q11.2 microdeletion syndrome establishes CRLF3 as a critical regulator of neurogenesis. Cell Rep. 2021;36:109315 pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using MA5-15854 anti-OTX2 antibody with DAB staining.

product information

Product Type :

Antibody

Product Name :

OTX2 Monoclonal Antibody (1H12C4B5)

Catalog # :

MA5-15854

Quantity :

100 µL

Price :

US 510

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Flow Cytometry: 1/200 - 1/400, Immunocytochemistry: 1:200-1:1000, Immunofluorescence: 1/200 - 1/1000, Immunohistochemistry: 1/200 - 1/1000, Western Blot: 1/500 - 1/2000

Species :

Human, Mouse, Rat

Clone :

1H12C4B5

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5-'TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).

Immunogen :

Purified recombinant fragment of human OTX2 expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1/200 - 1/400, Immunocytochemistry: 1:200-1:1000, Immunofluorescence: 1/200 - 1/1000, Immunohistochemistry: 1/200 - 1/1000, Western Blot: 1/500 - 1/2000

Aliases :

CPHD6; E130306E05Rik; homeobox Otx2; homeobox protein OTX2; MCOPS5; orthodenticle homeobox 2; orthodenticle homolog 2; OTX; OTX2

more info or order :

Invitrogen product webpage