GRK2 Monoclonal Antibody (3F8) | Invitrogen MA5-15840 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

GRK2 Monoclonal Antibody (3F8)

catalog :

MA5-15840

quantity :

100 µL

price :

US 516

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3F8

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:1000	Buchholz M, Honstein T, Kirchhoff S, Kreider R, Schmidt H, Sipos B, et al. A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer. PLoS ONE. 2015;10:e0122946 pubmed publisher
	Mayberry C, Wilczek M, Fong T, Nichols S, Maginnis M. GRK2 mediates β-arrestin interactions with 5-HT2 receptors for JC polyomavirus endocytosis. J Virol. 2021;: pubmed publisher

image

image 1 :

Immunofluorescence analysis of NIH/3T3 cells using MA5-15840 anti-GRK2 antibody (green). Blue staining is DRAQ5 fluorescent DNA dye.

image 2 :

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and cervical cancer tissues (right) using MA5-15840 anti-GRK2 antibody with DAB staining.

product information

Product Type :

Antibody

Product Name :

GRK2 Monoclonal Antibody (3F8)

Catalog # :

MA5-15840

Quantity :

100 µL

Price :

US 516

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

Immunocytochemistry: 1:200-1:1000, Immunofluorescence: 1/200 - 1/1000, Immunohistochemistry: 1/200 - 1/1000, Western Blot: 1/500 - 1/2000

Species :

Human, Mouse, Non-human primate, Rat

Clone :

3F8

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.

Immunogen :

Purified recombinant fragment of human GRK2 expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:200-1:1000, Immunofluorescence: 1/200 - 1/1000, Immunohistochemistry: 1/200 - 1/1000, Western Blot: 1/500 - 1/2000

Aliases :

ADRBK1; Adrbk-1; adrenergic beta receptor kinase 1; adrenergic receptor kinase, beta 1; adrenergic, beta, receptor kinase 1; BARK; BARK1; Bark-1; beta ARK; Beta-adrenergic receptor kinase 1; beta-adrenergic receptor kinase 1 beta ARK1; beta-AR kinase-1; betaARK1; BETA-ARK1; beta-ARK-1; EC 2.7.1.126; G protein-coupled receptor kinase 2; G-protein coupled receptor kinase 2; G-protein-coupled receptor kinase 2; grk 2; Grk2; GRK-2

more info or order :

Invitrogen product webpage