Alpha-Smooth Muscle Actin Monoclonal Antibody (1H8) | Invitrogen MA5-15805 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Alpha-Smooth Muscle Actin Monoclonal Antibody (1H8)

catalog :

MA5-15805

quantity :

100 µL

price :

US 476

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1H8

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse	McGowan S, McCoy D. Regulation of fibroblast lipid storage and myofibroblast phenotypes during alveolar septation in mice. Am J Physiol Lung Cell Mol Physiol. 2014;307:L618-31 pubmed publisher

image

image 1 :

Immunofluorescence analysis of NIH/3T3 cells using MA5-15805 anti-ACTA2 antibody (green). Blue staining is DRAQ5 fluorescent DNA dye.

product information

Product Type :

Antibody

Product Name :

Alpha-Smooth Muscle Actin Monoclonal Antibody (1H8)

Catalog # :

MA5-15805

Quantity :

100 µL

Price :

US 476

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

ELISA: 1:10,000, Flow Cytometry: 1/200 - 1/400, Immunocytochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Human, Mouse, Non-human primate, Rat

Clone :

1H8

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

Immunogen :

Purified recombinant fragment of human ACTA2 expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Flow Cytometry: 1/200 - 1/400, Immunocytochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

0610041G09Rik; AAT6; ACT-4; acta2; acta2.L; acta2.S; actin; Actin Alpha 2 Smooth Muscle Aorta; actin alpha 2, smooth muscle; Actin Vascular Smooth Muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha 2, smooth muscle, aorta L homeolog; actin, alpha 2, smooth muscle, aorta S homeolog; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; ACTSA; ACTVS; alpha actin 2; Alpha Cardiac Actin; alpha smooth muscle actin; alpha-actin; Alpha-actin-2; alpha-cardiac actin; alphaSMA; alpha-SM-actin; alpha-smooth muscle actin; Aortic Smooth Muscle; ASMA; a-sma; cell growth-inhibiting gene 46 protein; GIG46; Growth Inhibiting Gene 46; hypothetical protein LOC515610; LOW QUALITY PROTEIN: actin, aortic smooth muscle; mymy5; SM actin; sma; SMactin; sm-actin; SMalphaA; smooth muscle; smooth muscle alpha-actin; vascular smooth muscle alpha-actin; wu:fb63d03; XELAEV_18034370mg

more info or order :

Invitrogen product webpage