GAPDH Loading Control Monoclonal Antibody (GA1R), Biotin | Invitrogen MA5-15738-BTIN product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

GAPDH Loading Control Monoclonal Antibody (GA1R), Biotin

catalog :

MA5-15738-BTIN

quantity :

50 µL

price :

US 235.00

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

GA1R

reactivity :

Chinese hamsters, human, mouse, rat, chicken, domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 6h western blot; mouse; 1:1000; loading ...; fig 6h	Xu Y, Wang Y, Yao A, Xu Z, Dou H, Shen S, et al. Low Frequency Magnetic Fields Induce Autophagy-associated Cell Death in Lung Cancer through miR-486-mediated Inhibition of Akt/mTOR Signaling Pathway. Sci Rep. 2017;7:11776 pubmed publisher
	Chen Y, Liu J, Zhang W, Kadier A, Wang R, Zhang H, et al. O-GlcNAcylation Enhances NUSAP1 Stability and Promotes Bladder Cancer Aggressiveness. Onco Targets Ther. 2021;14:445-454 pubmed publisher
	Chen X, Zhang Q, Dang X, Song T, Wang Y, Yu Z, et al. Targeting the CtBP1-FOXM1 transcriptional complex with small molecules to overcome MDR1-mediated chemoresistance in osteosarcoma cancer stem cells. J Cancer. 2021;12:482-497 pubmed publisher
	Dey S, Kwon J, Liu S, Hodge G, Taleb S, Zimmers T, et al. miR-29a Is Repressed by MYC in Pancreatic Cancer and Its Restoration Drives Tumor-Suppressive Effects via Downregulation of LOXL2. Mol Cancer Res. 2020;18:311-323 pubmed publisher
	Nguyen T, Duchon A, Manousopoulou A, Loaëc N, Villiers B, Pani G, et al. Correction of cognitive deficits in mouse models of Down syndrome by a pharmacological inhibitor of DYRK1A. Dis Model Mech. 2018;11: pubmed publisher
	Tao Z, Zheng L, Smith C, Luo J, Robinson A, Almeida F, et al. Estradiol signaling mediates gender difference in visceral adiposity via autophagy. Cell Death Dis. 2018;9:309 pubmed publisher
	Wu Q, Dai Q, Jiang L, Wang Y, Yang T, Miao J, et al. Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats. Mol Med Rep. 2017;16:1578-1583 pubmed publisher
	Gainullin M, Zhukov I, Zhou X, Mo Y, Astakhova L, Ernberg I, et al. Degradation of cofilin is regulated by Cbl, AIP4 and Syk resulting in increased migration of LMP2A positive nasopharyngeal carcinoma cells. Sci Rep. 2017;7:9012 pubmed publisher
	Ortiz D, Glassbrook J, Pellett P. Protein-Protein Interactions Suggest Novel Activities of Human Cytomegalovirus Tegument Protein pUL103. J Virol. 2016;90:7798-810 pubmed publisher
	Treacy Abarca S, Mukherjee S. Legionella suppresses the host unfolded protein response via multiple mechanisms. Nat Commun. 2015;6:7887 pubmed publisher
	Meas R, Smerdon M, Wyrick J. The amino-terminal tails of histones H2A and H3 coordinate efficient base excision repair, DNA damage signaling and postreplication repair in Saccharomyces cerevisiae. Nucleic Acids Res. 2015;43:4990-5001 pubmed publisher

product information

Product Type :

Antibody

Product Name :

GAPDH Loading Control Monoclonal Antibody (GA1R), Biotin

Catalog # :

MA5-15738-BTIN

Quantity :

50 µL

Price :

US 235.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bacteria, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast

Applications :

ELISA: Assay-dependent, Flow Cytometry: 2 µg/test, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1:50-1:500, Western Blot: 1:500-1:1,000

Species :

Bacteria, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast

Clone :

GA1R

Isotype :

IgG1

Storage :

4° C, do not freeze

Description :

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

Immunogen :

Recombinant GAPDH

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Flow Cytometry: 2 µg/test, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1:50-1:500, Western Blot: 1:500-1:1,000

Aliases :

38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; bb02e05; cb350; cb609; CDABP0047; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; fb71f08; fk58c09; G3PD; G3PDH; GAPD; GAPD2; gapdh; GAPDH2; GAPDH-2; GAPDHS; Gapds; Gapd-s; glceraldehyde-3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; glyceraldehyde phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase (G3PDH); glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase GAPDH; glyceraldehyde-3-phosphate dehydrogenase like-17 protein; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; glyceraldehyde-3-phosphate dehydrogenase, testis-specific; glyceraldehyde-phosphate-dehydrogenase; glycerine aldehyde 3-phosphate dehydrogenase; HEL-S-162eP; HEL-S-278; HGNC:4141; HSD35; HSD-35; I79_001391; KNC-NDS6; LOW QUALITY PROTEIN: glyceraldehyde-3-phosphate dehydrogenase, testis-specific; mg:bb02e05; MGC128279 protein; MGC88685; multifunctional protein, glycolytic enzyme; OK/SW-cl.12; Peptidyl-cysteine S-nitrosylase GAPDH; similar to glyceraldehyde 3-phosphate dehydrogenase; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; Unknown (protein for IMAGE:8101613); unnamed protein product; wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908

more info or order :

Invitrogen product webpage