product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GAPDH Loading Control Monoclonal Antibody (GA1R)
catalog :
MA5-15738-1MG
quantity :
1 mg
price :
US 1156.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GA1R
reactivity :
Chinese hamsters, Helicobacter pylori, human, mouse, rat, dogs, chicken, domestic rabbit
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 354
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 4c
Wyżewski Z, Gregorczyk Zboroch K, Mielcarska M, Bossowska Nowicka M, Struzik J, Szczepanowska J, et al. Mitochondrial Heat Shock Response Induced by Ectromelia Virus is Accompanied by Reduced Apoptotic Potential in Murine L929 Fibroblasts. Arch Immunol Ther Exp (Warsz). 2019;67:401-414 pubmed publisher
  • western blot; mouse; 1:10,000; loading ...; fig 4f
Xiang J, Yang S, Xin N, Gaertig M, Reeves R, Li S, et al. DYRK1A regulates Hap1-Dcaf7/WDR68 binding with implication for delayed growth in Down syndrome. Proc Natl Acad Sci U S A. 2017;114:E1224-E1233 pubmed publisher
  • western blot; human; loading ...; fig 1a
Nguyen J, Bernert R, In K, Kang P, Sebastiao N, Hu C, et al. Gamma-interferon-inducible lysosomal thiol reductase is upregulated in human melanoma. Melanoma Res. 2016;26:125-37 pubmed publisher
  • western blot; human; 1:1000; fig 1a
Li Y, Banerjee S, Wang Y, Goldstein S, Dong B, Gaughan C, et al. Activation of RNase L is dependent on OAS3 expression during infection with diverse human viruses. Proc Natl Acad Sci U S A. 2016;113:2241-6 pubmed publisher
  • western blot; mouse; 1:5000; fig s3
Li F, Buck D, De Winter J, Kolb J, Meng H, Birch C, et al. Nebulin deficiency in adult muscle causes sarcomere defects and muscle-type-dependent changes in trophicity: novel insights in nemaline myopathy. Hum Mol Genet. 2015;24:5219-33 pubmed publisher
  • western blot; Helicobacter pylori; 1:5000
Yuan Y, Wu Q, Cheng G, Liu X, Liu S, Luo J, et al. Recombinant human lactoferrin enhances the efficacy of triple therapy in mice infected with Helicobacter pylori. Int J Mol Med. 2015;36:363-8 pubmed publisher
  • western blot; human
Wright J, Atwan Z, Morris S, Leppard K. The Human Adenovirus Type 5 L4 Promoter Is Negatively Regulated by TFII-I and L4-33K. J Virol. 2015;89:7053-63 pubmed publisher
  • western blot; human
Pasqualon T, Pruessmeyer J, Weidenfeld S, Babendreyer A, Groth E, Schumacher J, et al. A transmembrane C-terminal fragment of syndecan-1 is generated by the metalloproteinase ADAM17 and promotes lung epithelial tumor cell migration and lung metastasis formation. Cell Mol Life Sci. 2015;72:3783-801 pubmed publisher
  • western blot; mouse
Richardson E, Shukla S, Sweet D, Wearsch P, Tsichlis P, Boom W, et al. Toll-like receptor 2-dependent extracellular signal-regulated kinase signaling in Mycobacterium tuberculosis-infected macrophages drives anti-inflammatory responses and inhibits Th1 polarization of responding T cells. Infect Immun. 2015;83:2242-54 pubmed publisher
  • western blot; chicken
Jarosinski K, Donovan K, Du G. Expression of fluorescent proteins within the repeat long region of the Marek's disease virus genome allows direct identification of infected cells while retaining full pathogenicity. Virus Res. 2015;201:50-60 pubmed publisher
  • western blot; mouse
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Shi L, Aymonnier K, Wagner D. Neutrophil stimulation with citrullinated histone H4 slows down calcium influx and reduces NET formation compared with native histone H4. PLoS ONE. 2021;16:e0251726 pubmed publisher
Zhang S, Yuan B, Lam J, Zhou J, Zhou X, Ramos Mandujano G, et al. Structure of the full-length human Pannexin1 channel and insights into its role in pyroptosis. Cell Discov. 2021;7:30 pubmed publisher
Zhang M, Yang H, Chen Z, Hu X, Wu T, Liu W. Long Noncoding RNA X-Inactive-Specific Transcript Promotes the Secretion of Inflammatory Cytokines in LPS Stimulated Astrocyte Cell Via Sponging miR-29c-3p and Regulating Nuclear Factor of Activated T cell 5 Expression. Front Endocrinol (Lausanne). 2021;12:573143 pubmed publisher
Sun X, Lin F, Sun W, Zhu W, Fang D, Luo L, et al. Exosome-transmitted miRNA-335-5p promotes colorectal cancer invasion and metastasis by facilitating EMT via targeting RASA1. Mol Ther Nucleic Acids. 2021;24:164-174 pubmed publisher
Tao Z, Shi L, Parke J, Zheng L, Gu W, Dong X, et al. Sirt1 coordinates with ERα to regulate autophagy and adiposity. Cell Death Discov. 2021;7:53 pubmed publisher
Jones H, Heilig R, Fischer R, Kessler B, Pinto Fernandez A. ABPP-HT - High-Throughput Activity-Based Profiling of Deubiquitylating Enzyme Inhibitors in a Cellular Context. Front Chem. 2021;9:640105 pubmed publisher
Li Z, Liu J, Zhao S, Ma Q, Liu A, Li Y, et al. Theileria annulata Subtelomere-Encoded Variable Secreted Protein-TA05575 Binds to Bovine RBMX2. Front Cell Infect Microbiol. 2021;11:644983 pubmed publisher
Kang D, Shim K. Early heat exposure effect on the heat shock proteins in broilers under acute heat stress. Poult Sci. 2021;100:100964 pubmed publisher
Kar N, Ferguson D, Zhang N, Waldorff E, Ryaby J, DiDonato J. Pulsed-electromagnetic-field induced osteoblast differentiation requires activation of genes downstream of adenosine receptors A2A and A3. PLoS ONE. 2021;16:e0247659 pubmed publisher
Cho D, Lee S, Cho K. Anastral Spindle 3/Rotatin Stabilizes Sol narae and Promotes Cell Survival in Drosophila melanogaster. Mol Cells. 2021;44:13-25 pubmed publisher
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Grancieri M, Martino H, Gonzalez de Mejia E. Protein Digests and Pure Peptides from Chia Seed Prevented Adipogenesis and Inflammation by Inhibiting PPARγ and NF-κB Pathways in 3T3L-1 Adipocytes. Nutrients. 2021;13: pubmed publisher
Chakrapani B, Khan M, Kadumuri R, Gupta S, Verma M, Awasthi S, et al. The uncharacterized protein FAM47E interacts with PRMT5 and regulates its functions. Life Sci Alliance. 2021;4: pubmed publisher
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Meng X, Sun W, Yu J, Zhou Y, Gu Y, Han J, et al. LINC00460-miR-149-5p/miR-150-5p-Mutant p53 Feedback Loop Promotes Oxaliplatin Resistance in Colorectal Cancer. Mol Ther Nucleic Acids. 2020;22:1004-1015 pubmed publisher
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Pattanayak B, Garrido Cano I, Adam Artigues A, Tormo E, Pineda B, Cabello P, et al. MicroRNA-33b Suppresses Epithelial-Mesenchymal Transition Repressing the MYC-EZH2 Pathway in HER2+ Breast Carcinoma. Front Oncol. 2020;10:1661 pubmed publisher
Zhang J, Xiao C, Feng Z, Gong Y, Sun B, Li Z, et al. SOX4 promotes the growth and metastasis of breast cancer. Cancer Cell Int. 2020;20:468 pubmed publisher
Chou L, Chen C, Chuang S, Cheng T, Lin Y, Chou H, et al. Discoidin Domain Receptor 1 Regulates Runx2 during Osteogenesis of Osteoblasts and Promotes Bone Ossification via Phosphorylation of p38. Int J Mol Sci. 2020;21: pubmed publisher
Berry J, Munoz L, Rodríguez Stewart R, Selvaraj P, Mainou B. Doxorubicin Conjugation to Reovirus Improves Oncolytic Efficacy in Triple-Negative Breast Cancer. Mol Ther Oncolytics. 2020;18:556-572 pubmed publisher
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Knittel V, Sadana P, Seekircher S, Stolle A, Körner B, Volk M, et al. RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression. PLoS Pathog. 2020;16:e1008552 pubmed publisher
Barrera A, Ramos H, Vera Otarola J, Fernández García L, Angulo J, Olguín V, et al. Post-translational modifications of hnRNP A1 differentially modulate retroviral IRES-mediated translation initiation. Nucleic Acids Res. 2020;48:10479-10499 pubmed publisher
Vallecillo Zúniga M, Rathgeber M, Poulson P, Hayes S, Luddington J, Gill H, et al. Treatment with galectin-1 improves myogenic potential and membrane repair in dysferlin-deficient models. PLoS ONE. 2020;15:e0238441 pubmed publisher
Gandalovičová A, Šůchová A, Cermák V, Merta L, Rosel D, Brabek J. Sustained Inflammatory Signalling through Stat1/Stat2/IRF9 Is Associated with Amoeboid Phenotype of Melanoma Cells. Cancers (Basel). 2020;12: pubmed publisher
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Zhao Y, Ye X, Shehata M, Dunker W, Xie Z, Karijolich J. The RNA quality control pathway nonsense-mediated mRNA decay targets cellular and viral RNAs to restrict KSHV. Nat Commun. 2020;11:3345 pubmed publisher
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Bohin N, McGowan K, Keeley T, Carlson E, Yan K, Samuelson L. Insulin-like Growth Factor-1 and mTORC1 Signaling Promote the Intestinal Regenerative Response After Irradiation Injury. Cell Mol Gastroenterol Hepatol. 2020;10:797-810 pubmed publisher
Cermák V, Gandalovičová A, Merta L, Harant K, Rosel D, Brabek J. High-throughput transcriptomic and proteomic profiling of mesenchymal-amoeboid transition in 3D collagen. Sci Data. 2020;7:160 pubmed publisher
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Wang G, Kostidis S, Tiemeier G, Sol W, De Vries M, Giera M, et al. Shear Stress Regulation of Endothelial Glycocalyx Structure Is Determined by Glucobiosynthesis. Arterioscler Thromb Vasc Biol. 2020;40:350-364 pubmed publisher
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Whelan J, Li Y, Silverman R, Weiss S. Zika virus production is resistant to RNase L antiviral activity. J Virol. 2019;: pubmed publisher
Giacalone J, Andorf J, Zhang Q, Burnight E, Ochoa D, Reutzel A, et al. Development of a Molecularly Stable Gene Therapy Vector for the Treatment of RPGR-associated X-linked Retinitis Pigmentosa. Hum Gene Ther. 2019;: pubmed publisher
Cui Q, Cai C, Gao H, Ren L, Ji N, Gupta P, et al. Glesatinib, a c-MET/SMO Dual Inhibitor, Antagonizes P-glycoprotein Mediated Multidrug Resistance in Cancer Cells. Front Oncol. 2019;9:313 pubmed publisher
Narasimhan G, Henderson J, Luong H, Rajasekaran N, Qin G, Zhang J, et al. OBG-like ATPase 1 inhibition attenuates angiotensin II-induced hypertrophic response in human ventricular myocytes via GSK-3beta/beta-catenin signalling. Clin Exp Pharmacol Physiol. 2019;: pubmed publisher
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product information
Product Type :
Antibody
Product Name :
GAPDH Loading Control Monoclonal Antibody (GA1R)
Catalog # :
MA5-15738-1MG
Quantity :
1 mg
Price :
US 1156.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Bacteria, Canine, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast
Applications :
ELISA: Assay-dependent, Flow Cytometry: 2 µg/test, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1:50-1:500, Western Blot: 1:1,000-1:10,000
Species :
Bacteria, Canine, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast
Clone :
GA1R
Isotype :
IgG1
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
Immunogen :
Recombinant GAPDH
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Flow Cytometry: 2 µg/test, Immunocytochemistry: 1:20-1:200, Immunohistochemistry (Paraffin): 1:50-1:500, Western Blot: 1:1,000-1:10,000
Aliases :
38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; bb02e05; cb350; cb609; CDABP0047; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; fb71f08; fk58c09; G3PD; G3PDH; GAPD; GAPD2; gapdh; GAPDH2; GAPDH-2; GAPDHS; Gapds; Gapd-s; glceraldehyde-3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; glyceraldehyde phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase (G3PDH); glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase GAPDH; glyceraldehyde-3-phosphate dehydrogenase like-17 protein; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; glyceraldehyde-3-phosphate dehydrogenase, testis-specific; glyceraldehyde-phosphate-dehydrogenase; glycerine aldehyde 3-phosphate dehydrogenase; HEL-S-162eP; HEL-S-278; HGNC:4141; HSD35; HSD-35; I79_001391; KNC-NDS6; LOW QUALITY PROTEIN: glyceraldehyde-3-phosphate dehydrogenase, testis-specific; mg:bb02e05; MGC128279 protein; MGC88685; multifunctional protein, glycolytic enzyme; OK/SW-cl.12; Peptidyl-cysteine S-nitrosylase GAPDH; similar to glyceraldehyde 3-phosphate dehydrogenase; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; Unknown (protein for IMAGE:8101613); unnamed protein product; wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA