Ki-67 Monoclonal Antibody (8D5) | Invitrogen MA5-15690 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Ki-67 Monoclonal Antibody (8D5)

catalog :

MA5-15690

quantity :

100 uL

price :

US 542.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8D5

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; rat; loading ...; fig s2	Mehta N, Lyon J, Patil K, Mokarram N, Kim C, Bellamkonda R. Bacterial Carriers for Glioblastoma Therapy. Mol Ther Oncolytics. 2017;4:1-17 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig 2	Guan C, Zhang J, Zhang J, Shi H, Ni R. Enhanced expression of early mitotic inhibitor-1 predicts a poor prognosis in esophageal squamous cell carcinoma patients. Oncol Lett. 2016;12:114-120 pubmed
western blot; human; fig 9c	Tzenaki N, Aivaliotis M, Papakonstanti E. Focal adhesion kinase phosphorylates the phosphatase and tensin homolog deleted on chromosome 10 under the control of p110Î´ phosphoinositide-3 kinase. FASEB J. 2015;29:4840-52 pubmed publisher
	Liu Z, Krstic A, Neve A, Casalou C, Rauch N, Wynne K, et al. Kinase Suppressor of RAS 1 (KSR1) Maintains the Transformed Phenotype of BRAFV600E Mutant Human Melanoma Cells. Int J Mol Sci. 2023;24: pubmed publisher
	Gu S, Qian L, Liu Y, Miao J, Shen H, Zhang S, et al. Upregulation of long non-coding RNA MYU promotes proliferation, migration and invasion of esophageal squamous cell carcinoma cells. Exp Ther Med. 2021;21:644 pubmed publisher
	Kothe T, Royse E, Kemp M, Schmidt A, Salomone F, Saito M, et al. Effects of budesonide and surfactant in preterm fetal sheep. Am J Physiol Lung Cell Mol Physiol. 2018;315:L193-L201 pubmed publisher
	Kavanagh E, Lindsay S, Halasz M, Gubbins L, Weiner Gorzel K, Guang M, et al. Protein and chemotherapy profiling of extracellular vesicles harvested from therapeutic induced senescent triple negative breast cancer cells. Oncogenesis. 2017;6:e388 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Ki-67 Monoclonal Antibody (8D5)

Catalog # :

MA5-15690

Quantity :

100 uL

Price :

US 542.00

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: 1:10,000, Immunocytochemistry: 1:200-1:500, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Human

Clone :

8D5

Isotype :

IgG1

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

Immunogen :

Synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH (aa 1100-1113).

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Immunocytochemistry: 1:200-1:500, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05

more info or order :

Invitrogen product webpage