N-cadherin Monoclonal Antibody (5D5) | Invitrogen MA5-15633 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

N-cadherin Monoclonal Antibody (5D5)

catalog :

MA5-15633

quantity :

100 µL

price :

US 504

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5D5

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000	Chen F, Zhuang M, Peng J, Wang X, Huang T, Li S, et al. Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF-Î² signaling pathway. Mol Med Rep. 2014;10:1999-2003 pubmed publisher
	Shishkova D, Velikanova E, Bogdanov L, Sinitsky M, Kostyunin A, Tsepokina A, et al. Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular Inflammation. Int J Mol Sci. 2021;22: pubmed publisher
	Shishkova D, Markova V, Sinitsky M, Tsepokina A, Velikanova E, Bogdanov L, et al. Calciprotein Particles Cause Endothelial Dysfunction under Flow. Int J Mol Sci. 2020;21: pubmed publisher
	Shishkova D, Markova V, Sinitsky M, Tsepokina A, Frolov A, Zagorodnikov N, et al. Co-Culture of Primary Human Coronary Artery and Internal Thoracic Artery Endothelial Cells Results in Mutually Beneficial Paracrine Interactions. Int J Mol Sci. 2020;21: pubmed publisher
	Kutikhin A, Tupikin A, Matveeva V, Shishkova D, Antonova L, Kabilov M, et al. Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature. Cells. 2020;9: pubmed publisher
	Alcantara K, Garcia R. MicroRNA‑92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells. Oncol Rep. 2019;41:2103-2116 pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer(D), using CDH2 antibody with DAB staining.

product information

Product Type :

Antibody

Product Name :

N-cadherin Monoclonal Antibody (5D5)

Catalog # :

MA5-15633

Quantity :

100 µL

Pricing :

US 504

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 1:10,000, Flow Cytometry: 1/200 - 1/400, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Human, Mouse, Rat

Clone :

5D5

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

Immunogen :

Purified recombinant fragment of human CDH2 expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Flow Cytometry: 1/200 - 1/400, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

bib; cadherin 2; cadherin 2 L homeolog; cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, neuronal; cadherin 2, type 1 preproprotein; cadherin 2, type 1, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal) L homeolog; cadherin 2, type 1, N-cadherin a (neuronal); Cadherin2; cadherin-2; Cadherin-2A; Cadherin-N; calcium-dependent adhesion protein, neuronal; CD325; CDH2; cdh2.L; cdh2a; cdh2-a; cdh2-b; CDHN; cdhn-a; CDw325; CDw325 antigen; cell adhesion molecule; glass onion; glo; I79_014914; labyrinth; lyr; NCAD; N-Cad; N-cadherin; N-cadherin 1; N-cadherin A; N-cadherin precursor; N-cadherin-2; neural cadherin; Neural cadherin A; neural cadherin-2; neural calcium-dependent cell adhesion molecule; neural-cadherin; pac; Parachute; unnamed protein product; wu:fb47h04; XELAEV_18031953mg; ZNCAD

more info or order :

Invitrogen product webpage