This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
RAG2 Monoclonal Antibody (4D5)
catalog :
MA5-15622
quantity :
100 uL
price :
US 480.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4D5

The same clone is also sold as:
reactivity :
human
application :
western blot, ELISA
product information
Product Type :
Antibody
Product Name :
RAG2 Monoclonal Antibody (4D5)
Catalog # :
MA5-15622
Quantity :
100 uL
Price :
US 480.00
Clonality :
Monoclonal
Host :
Mouse
Reactivity :
Human
Applications :
ELISA: 1:10,000, Western Blot: 1:500-1:2,000
Species :
Human
Clone :
4D5
Isotype :
IgG1
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
RAG2 (V(D)J recombination-activating protein 2) is a core component of the RAG complex - a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity) and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: 1) first a nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends. 2) hairpin coding ends and 2 blunt, 5'-phosphorylated ends are created. The chromatin structure plays an essential rold in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and hair pinning expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin. This prevents accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. Mutations affecting the gene can results in combined cellular and humoral immune defects with granulomas, severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive, and Omenn syndrome.
Immunogen :
Purified recombinant fragment of human RAG2(350-527aa) expressed in E. Coli.
Format :
Liquid
Applications w/Dilutions :
ELISA: 1:10,000, Western Blot: 1:500-1:2,000
Aliases :
RAG 2; Rag2; RAG-2; recombinase activating gene 2; recombination activating gene 2; recombination activating protein 2; V(D)J recombination-activating protein 2
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA