HSPA4 Monoclonal Antibody (5A6) | Invitrogen MA5-15578 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HSPA4 Monoclonal Antibody (5A6)

catalog :

MA5-15578

quantity :

100 µL

price :

US 457

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5A6

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human	Chow A, Mok P, Xiao D, Khalouei S, Brown I. Heteromeric complexes of heat shock protein 70 (HSP70) family members, including Hsp70B', in differentiated human neuronal cells. Cell Stress Chaperones. 2010;15:545-53 pubmed publisher

image

image 1 :

Immunofluorescence analysis of NIH/3T3 cells using HSP70 antibody (green). Blue: DRAQ5 fluorescent DNA dye.

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast cancer using HSP70 antibody with DAB staining.

product information

Product Type :

Antibody

Product Name :

HSPA4 Monoclonal Antibody (5A6)

Catalog # :

MA5-15578

Quantity :

100 µL

Pricing :

US 457

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ELISA: 1:10,000, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Human, Mouse

Clone :

5A6

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.

Immunogen :

Purified recombinant fragment of human HSP70 expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

70kDa; AI317151; APG2; APG-2; epididymis secretory sperm binding protein Li 5a; heat shock 70 kDa protein 4; heat shock 70kD protein 4; heat shock 70kDa protein 4; Heat shock 70-related protein APG-2; Heat shock protein; heat shock protein 4; heat shock protein Apg-2; heat shock protein family A (Hsp70) member 4; heat shock protein, 110 kDa; HEL-S-5a; HS24/P52; Hsp110; hsp70; hsp70 RY; Hsp70RY; Hspa4; HSPH2; Irp94; ischemia responsive 94 kDa protein; mKIAA4025; RY

more info or order :

Invitrogen product webpage