PTEN Monoclonal Antibody (1B8) | Invitrogen MA5-15560 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PTEN Monoclonal Antibody (1B8)

catalog :

MA5-15560

quantity :

100 µL

price :

US 457

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1B8

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; fig s5a	Wei J, Long L, Zheng W, Dhungana Y, Lim S, Guy C, et al. Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy. Nature. 2019;576:471-476 pubmed publisher

image

image 1 :

Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using PTEN antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

product information

Product Type :

Antibody

Product Name :

PTEN Monoclonal Antibody (1B8)

Catalog # :

MA5-15560

Quantity :

100 µL

Pricing :

US 457

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ELISA: 1:10,000, Flow Cytometry: 1/200 - 1/400, Immunocytochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Human, Mouse

Clone :

1B8

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.

Immunogen :

Purified recombinant fragment of PTEN expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Flow Cytometry: 1/200 - 1/400, Immunocytochemistry: 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

10q23del; 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; BZS; CWS1; DEC; GLM2; homolog of human mutated in multiple advanced cancers; MHAM; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; Mmac; MMAC1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; phosphatase and tensin homolog (mutated in multiple advanced cancers 1); phosphatase and tensin homolog deleted on chromosome ten; phosphatase and tensin-like protein; phosphatidylinositol 3; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; protein tyrosine phosphatase and tensin-like protein; PTEN; pten tumor suppressor; PTEN1; TEP1

more info or order :

Invitrogen product webpage