Ki-67 Monoclonal Antibody (4A1) | Invitrogen MA5-15525 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Ki-67 Monoclonal Antibody (4A1)

catalog :

MA5-15525

quantity :

100 µL

price :

US 498

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4A1

reactivity :

human

application :

ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; loading ...; fig 5b	Ling C, Nishimoto K, Rolfs Z, Smith L, Frey B, Welham N. Differentiated fibrocytes assume a functional mesenchymal phenotype with regenerative potential. Sci Adv. 2019;5:eaav7384 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200; fig 1	Hasby E. Mammary serine protease inhibitor and CD138 immunohistochemical expression in ovarian serous and clear cell carcinomas. Tumour Biol. 2016;37:4889-900 pubmed publisher
immunohistochemistry; human; 1:200	Hu W, Liu T, Ivan C, Sun Y, Huang J, Mangala L, et al. Notch3 pathway alterations in ovarian cancer. Cancer Res. 2014;74:3282-93 pubmed publisher
immunohistochemistry; human	Qiu Z, Wang L, Han J, Liu D, Huang W, Altaf K, et al. Prognostic impact of Raf-1 and p-Raf-1 expressions for poor survival rate in non-small cell lung cancer. Cancer Sci. 2012;103:1774-9 pubmed publisher
	Wang L, Yang C, Wang Q, Liu Q, Wang Y, Zhou J, et al. Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma. Theranostics. 2020;10:6674-6694 pubmed publisher

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human lymph node (A), esophagus (B), lung cancer (C), rectum cancer (D), showing nuclear localization using KI67 antibody with DAB staining.

product information

Product Type :

Antibody

Product Name :

Ki-67 Monoclonal Antibody (4A1)

Catalog # :

MA5-15525

Quantity :

100 µL

Pricing :

US 498

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: 1:10,000, Immunocytochemistry: 1:100, Immunohistochemistry: 1:200-1:1,000

Species :

Human

Clone :

4A1

Isotype :

IgG2b

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

Immunogen :

Synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Immunocytochemistry: 1:100, Immunohistochemistry: 1:200-1:1,000

Aliases :

antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05

more info or order :

Invitrogen product webpage