B-Raf Monoclonal Antibody (1H12) | Invitrogen MA5-15495 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

B-Raf Monoclonal Antibody (1H12)

catalog :

MA5-15495

quantity :

100 µL

price :

US 486

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1H12

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

citations: 2

Reference
Gillies T, Pargett M, Silva J, Teragawa C, McCormick F, Albeck J. Oncogenic mutant RAS signaling activity is rescaled by the ERK/MAPK pathway. Mol Syst Biol. 2020;16:e9518 pubmed publisher
Martens S, Jeong M, Tonnus W, Feldmann F, Hofmans S, Goossens V, et al. Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury. Cell Death Dis. 2017;8:e2904 pubmed publisher

product information

Product Type :

Antibody

Product Name :

B-Raf Monoclonal Antibody (1H12)

Catalog # :

MA5-15495

Quantity :

100 µL

Price :

US 486

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

ELISA: 1:10,000, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000

Species :

Human, Mouse

Clone :

1H12

Isotype :

IgG1

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neuron. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in B-Raf have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for B-Raf.

Immunogen :

Purified recombinant fragment of human BRAF expressed in E. Coli.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry (Paraffin): 1:200-1:1,000, Western Blot: 1:500-1:2,000

Aliases :

94 kDa B-raf protein; 9930012E13Rik; AA120551; AA387315; AA473386; Braf; B-raf; B-raf protein; B-raf protein isoform 1; B-raf protein isoform 2; B-Raf proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf proto-oncogene, serine/threonine kinase; Braf transforming gene; BRAF1; B-RAF1; Braf2; Braf-2; C230098H17; C87398; C-RMIL; D6Ertd631e; FLJ95109; MGC126806; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; Proto-oncogene B-Raf; proto-oncogene c-Rmil; RAFB1; RMIL; rmil serine/threonine-protein kinase; serine/threonine kinase; serine/threonine-protein kinase B-raf; Serine/threonine-protein kinase Rmil; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1